Polysaccharide of Atractylodes macrocephala Koidz Relieves Lipopolysaccharide-Induced Apoptosis through Mitochondrial and Death Receptor Pathways in Thymus of Goslings

CHEN Lijun; LI Bingxin; CAO Nan; FU Xinliang; YANG Baohe; YUAN Mingfeng; XU Danning; TIAN Yunbo; HUANG Yunmao; LI Wanyan

doi:10.3969/j.issn.1006-267x.2022.12.052

2022 , Vol. 34 >Issue 12: 8049 - 8060

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.052

RESEARCH PAPER

Polysaccharide of Atractylodes macrocephala Koidz Relieves Lipopolysaccharide-Induced Apoptosis through Mitochondrial and Death Receptor Pathways in Thymus of Goslings

CHEN Lijun ,
LI Bingxin ,
CAO Nan ,
FU Xinliang ,
YANG Baohe ,
YUAN Mingfeng ,
XU Danning ,
TIAN Yunbo ,
HUANG Yunmao ,
LI Wanyan

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1. College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
2. Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China;
3. Yunnan Kuaidaduo Animal Husbandry Technology Co., Ltd., Yuxi 653100, China

Received date: 2022-05-20

Online published: 2022-12-15

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Abstract

This experiment was conducted to study the effects of polysaccharide of Atractylodes macrocephala Koidz (PAMK) on oxidative stress, inflammatory factors and apoptosis level in thymus of goslings treated with lipopolysaccharide (LPS), to find that whether PAMK relieved LPS-induced apoptosis through the mitochondrial and death receptor pathways in thymus of goslings. A total of 80 one-day-old healthy goslings were selected and randomly divided into 4 groups with 5 replicates per group and 4 goslings per replicate. Goslings in control group (CON group) and lipopolysaccharide group (LPS group) were fed the basal diets, and others in polysaccharide of Atractylodes macrocephala Koidz group (PAMK group) and lipopolysaccharide+ polysaccharide of Atractylodes macrocephala Koidz group (LPS+PAMK group) were fed basal diets supplemented with 400 mg/kg PAMK. Goslings in CON group and PAMK group were intraperitoneal injected 1 mL normal saline on days 24, 26 and 28, respectively, and goslings in LPS group and LPS+PAMK group were intraperitoneal injected 2 mg/kg LPS solution on days 24, 26 and 28, respectively. The experiment lasted for 28 days. The results showed as follows:1) the hematoxylin-eosin staining and ultrastructural observation showed that PAMK relieved the LPS-induced injury and apoptosis in thymus. 2) Compared with the CON group, the mRNA relative expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) of PAMK group were no significant difference (P>0.05), and the interleukin-10 (IL-10) mRNA relative expression level was significantly decreased (P<0.05). Compared with the LPS group, the mRNA relative expression levels of IL-1β, IL-6, TNF-α and IL-10 of LPS+PAMK group were significantly decreased (P<0.05). 3) Compared with the LPS group, the mRNA relative expression levels of catalase (CAT), superoxide dismutase (SOD) of LPS+PAMK group were significantly increased (P<0.05), and the glutathione peroxidase (GPX) mRNA relative expression level was significantly decreased (P<0.05). 4) Compared with the CON group, the mRNA relative expression levels of B-cell lymphoma/leukaemia-2-associated X protein (Bax) and tumor protein 63 (TP63) of PAMK group were no significant difference (P>0.05), the cysteinyl aspartate specific proteinase-3 (Caspase-3) mRNA relative expression level was significantly decreased (P<0.05), and the mRNA relative expression levels of B-cell lymphoma/leukaemia-2 (Bcl-2) and cysteinyl aspartate specific proteinase-8 (Caspase-8) were significantly increased (P<0.05). Compared with the LPS group, the mRNA relative expression levels of Bax and Caspase-3 of LPS+PAMK group were significantly decreased (P<0.05), and the mRNA relative expression levels of Bcl-2, Caspase-8 and TP63 were significantly increased (P<0.05). 5) Compared with the CON group, the protein relative expression levels of Bax and Caspase-8 of PAMK group were no significant difference (P>0.05), and the Bcl-2 protein relative expression level was significantly decreased (P<0.05). Compared with the LPS group, the protein relative expression levels of Bax and Caspase-8 of LPS+PAMK group were significantly decreased (P<0.05), and the Bcl-2 protein relative expression level was significantly increased (P<0.05). In conclusion, the PAMK can inhibit the increase of oxidative stress and inflammatory factor levels induced by LPS, alleviate the thymus apoptosis of goslings through the mitochondrial and death receptor pathways, and maintain the structural integrity of thymus tissue.

Key words： polysaccharide of Atractylodes macrocephala Koidz; lipopolysaccharide; apoptosis; thymus; goslings

Cite this article

CHEN Lijun , LI Bingxin , CAO Nan , FU Xinliang , YANG Baohe , YUAN Mingfeng , XU Danning , TIAN Yunbo , HUANG Yunmao , LI Wanyan . Polysaccharide of Atractylodes macrocephala Koidz Relieves Lipopolysaccharide-Induced Apoptosis through Mitochondrial and Death Receptor Pathways in Thymus of Goslings[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 8049 -8060 . DOI: 10.3969/j.issn.1006-267x.2022.12.052

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