Regulation Effects of Carnosine and β-Alanine on Impaired Energy Metabolism induced by Dexamethasone in C2C12 Cells

LU Guwei; GAO Zhen; ZANG Yitian; MAO Kang; OUYANG Kehui; LI Yanjiao

doi:10.3969/j.issn.1006-267x.2022.12.053

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 12: 8061 - 8071

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.053

RESEARCH PAPER

Regulation Effects of Carnosine and β-Alanine on Impaired Energy Metabolism induced by Dexamethasone in C2C12 Cells

LU Guwei ,
GAO Zhen ,
ZANG Yitian ,
MAO Kang ,
OUYANG Kehui ,
LI Yanjiao

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Jiangxi Province Key Laboratory of Animal Nutrition/Animal Nutrition and Feed Safety Innovation Team, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchan 330045, China

Received date: 2022-05-31

Online published: 2022-12-15

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Abstract

The experiment was conducted to study the regulation effects of carnosine and β-alanine on impaired energy metabolism induced by dexamethasone in C2C12 cells. The C2C12 cells were seeded in 6-well culture plates, after 5 to 6 days of differentiation, the myoblasts were form myotubes and divided into control group (normal incubation for 36 h), stress group (DEX group, normal incubation for 24 h+10 μmol/L DEX incubation for 12 h), carnosine group (Car group, 10 mmol/L carnosine incubation for 24 h+10 μmol/L DEX incubation for 12 h) and β-alanine group (β-Ala group, 10 mmol/L β-alanine incubation for 24 h+10 μmol/L DEX incubation for 12 h). After the end of cell culture, cells were collected to determine the intracellular ATP, Ca²⁺ content and glycolytic metabolism and mitochondria-related enzyme activities. The results showed that compared with the control group, the contents of ATP, glucose and lactate and the activities of Ca²⁺-ATPase, lactate dehydrogenase and pyruvate kinase in cells of DEX group were significantly decreased (P<0.05 or P<0.01), while the contents of glycogen and Ca²⁺ and activities of hexokinase and mitochondrial respiratory chain enzyme complexes Ⅳ were significantly increased (P<0.05 or P<0.01). Compared with the DEX group, the contents of glucose and ATP and activities of phosphofructokinase and lactate dehydrogenase in cells of Car group were significantly increased the (P<0.05 or P<0.01), while the Ca²⁺ content and hexokinase activity were significantly decreased (P<0.05 or P<0.01); the contents of glucose and ATP and activities of phosphofructokinase and lactate dehydrogenase in cells of β-Ala group were significantly increased the (P<0.05 or P<0.01), while the contents of glycogen and Ca²⁺ and activity of hexokinase were significantly decreased (P<0.05 or P<0.01). In conclusion, carnosine and β-alanine can alleviate the energy metabolism injury induced by dexamethasone in mouse C2C12 cells by regulating glycolytic metabolism, but has no effect on regulating mitochondrial dysfunction.

Key words： carnosine; β-alanine; C2C12 cells; stress; energy metabolism

Cite this article

LU Guwei , GAO Zhen , ZANG Yitian , MAO Kang , OUYANG Kehui , LI Yanjiao . Regulation Effects of Carnosine and β-Alanine on Impaired Energy Metabolism induced by Dexamethasone in C2C12 Cells[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 8061 -8071 . DOI: 10.3969/j.issn.1006-267x.2022.12.053

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