Simultaneous Analysis of 8 Water Soluble Vitamins in Pet Feed

YAN Hua; LI Lan; WANG Jitong; JIA Zheng; YAO Ting; LIU Jia; XU Siyuan; TIAN Jing; FAN Xia

doi:10.3969/j.issn.1006-267x.2022.12.054

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 12: 8072 - 8085

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.054

RESEARCH PAPER

Simultaneous Analysis of 8 Water Soluble Vitamins in Pet Feed

YAN Hua ,
LI Lan ,
WANG Jitong ,
JIA Zheng ,
YAO Ting ,
LIU Jia ,
XU Siyuan ,
TIAN Jing ,
FAN Xia

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1. Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Beijing Veterinary Drug and Feed Monitoring Center, Beijing 102200, China

Received date: 2022-05-31

Online published: 2022-12-15

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Abstract

The aim of this study was to establish a method for the simultaneous analysis of 8 water soluble vitamins (vitamin B₁, vitamin B₂, vitamin B₆, niacin, nicotinamide, D-pantothenic acid, D-biotin and folic acid) in pet compound feed. In the experiment, the isotope internal standard was added to the pet compound feed samples, the samples were water bathed 40 min at 50℃ with 1% hydrochloric acid solution and the pH of the solutions were then adjusted to 6.0 by 10% ammonia solution. After precipitation, the supernatant was taken for analysis. The 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile solution were used as mobile phase, SB-AQ C18 chromatographic column (100 mm×3.0 mm, 1.8 μm) was used as the separation column, and gradient elution was performed. The eluate was ionized by electrospray ion source in positive ion mode, and the data were collected in multiple reaction monitoring mode, and quantified by internal standard method. The results showed that the eight water soluble vitamins had a good linear relationship in the range of 5.0 to 2 000 ng/mL, and the correlation coefficient (R²) was greater than 0.995 0. The recovery rates of dog food and cat food were 80.0% to 117.3% and 81.0% to 112.2%, respectively, and the relative standard deviations were 1.04% to 5.28% and 1.06% to 10.20%, respectively. The method is simple, rapid, sensitive and accurate, and can be used for the simultaneous detection of 8 water soluble vitamins in pet compound feed.

Key words： pet compound feed; water soluble vitamins; liquid chromatography-isotope dilution mass spectrometry; internal standard method

Cite this article

YAN Hua , LI Lan , WANG Jitong , JIA Zheng , YAO Ting , LIU Jia , XU Siyuan , TIAN Jing , FAN Xia . Simultaneous Analysis of 8 Water Soluble Vitamins in Pet Feed[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 8072 -8085 . DOI: 10.3969/j.issn.1006-267x.2022.12.054

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