RESEARCH PAPER

Effects of Chronic Cold Exposure on Growth Performance, Serum Biochemical Indices and Liver Health of Mice

  • QUAN Xin ,
  • HU Yajie ,
  • FAN Wenxuan ,
  • ZHANG Xirui ,
  • ZHOU Wanhui ,
  • ZHANG Meng ,
  • ZHANG Boxi ,
  • XU Bin ,
  • YANG Yuying ,
  • LI Shize
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  • Heilong Jiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, The National Experimental Teaching Demonstration Center of Veterinary Medcine, College of Animal Science Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2022-04-18

  Online published: 2022-12-15

Abstract

The aim of this test was to examine the chronic cold exposure on growth performance, serum biochemical indices and liver health of mice. Thirty-two 8-week-old C57BL/6 mice weighing (22±1) g were randomly divided into two groups:the control group[(24.0±0.5)℃] and the cold exposure group[(4±1)℃], with four replicates of four mice in each group. The cold exposure treatment was performed for three weeks at 3 h per day, followed by hematoxylin-eosin (HE) and Schiff's iodate (PAS) tissue staining, serum biochemical indices assay, Western Blot for expression of relevant apoptotic proteins, and growth performance assays. The results showed as follows:1) compared with the control group, the average daily feed intake in cold exposure group was extremely significantly increased (P<0.01), and the average daily gain in cold exposure group was significantly decreased (P<0.05). 2) HE staining showed that compared with the control group, the cold exposure group showed more polygenic cells and unevenly arranged cells, suggesting hepatocyte damage. The results of PAS staining showed that compared with the control group, the content of hepatic glycogen in the cold exposure group was decreased. 3) Compared with control group, aspartate aminotransferase (AST) activity in serum was significantly decreased in the cold exposure group (P<0.05) and inorganic phosphorus content was highly significant (P<0.01). 4) Compared with control group, the B-cell lymphoma-2 (Bcl-2)/Bcl-2-associated X protein (Bax) ratio (Bcl-2/Bax) was significantly higher in the liver of mice in cold exposure group (P<0.01). It can be seen that when the organism is exposed to cold, for adapt to the cold environment and maintain a normal energy supply, energy metabolism increases, which can have a certain impact on growth performance, and liver glycogen is broken down in large quantities in the liver to protect liver cells from apoptosis.

Cite this article

QUAN Xin , HU Yajie , FAN Wenxuan , ZHANG Xirui , ZHOU Wanhui , ZHANG Meng , ZHANG Boxi , XU Bin , YANG Yuying , LI Shize . Effects of Chronic Cold Exposure on Growth Performance, Serum Biochemical Indices and Liver Health of Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 8120 -8128 . DOI: 10.3969/j.issn.1006-267x.2022.12.058

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