RESEARCH PAPER

Effects of Fecal Microbiota Transplantation of Free Range and Captive Saba Pigs on Growth Performance, Nutrient Apparent Digestibility and Intestinal Morphology in Mice

  • CHENG Yandong ,
  • HU Tiannian ,
  • YIN Huajuan ,
  • CHA Guanghui ,
  • WANG Pu ,
  • YANG Kai ,
  • YE Pengfei ,
  • PAN Qiong ,
  • LIN Qiuye ,
  • CAO Zhenhui
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  • 1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    2. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received date: 2022-05-24

  Online published: 2022-12-15

Abstract

This study aimed to investigate the effects of fecal microbiota transplantation (FMT) of free range and captive Saba pigs on growth performance, nutrient apparent digestibility, lipid metabolism and intestinal morphology in Kunming mice. Ten healthy adult free range or captive Saba pigs were selected as FMT donors to prepare fecal bacteria solution. Then 30 healthy male Kunming mice were selected as FMT recipients and randomly divided into 5 groups with 6 mice in each group. Mice in 5 groups were gavaged with normal saline (CON group), low-dose FMT bacterial solution of free range Saba pig (SY-L group), medium-dose FMT bacterial solution of free range Saba pig (SY-M group), high-dose FMT bacterial solution of free range Saba pig (SY-H group) and medium-dose FMT bacterial solution of captive Saba pig (JY-M group), respectively, for 14 days. The experiment lasted for 28 days. The results showed as follows:1) compared with CON group, the average daily gain (ADG) in all FMT groups was increased and the feed to gain ratio (F/G) was decreased, and the difference was significant in SY-M group (P<0.05); compared with JY-M group, the ADG in SY-M group was significantly increased (P<0.05), and the F/G was significantly decreased (P<0.05). 2) Compared with CON group, the apparent digestibility of crude protein and ether extract in free range FMT groups was significantly increased (P<0.05); compared with JY-M group, the crude protein apparent digestibility in SY-M group was increased by 10.32% (P<0.05). 3) Compared with CON group, the contents of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in serum in all FMT groups were decreased, and the difference was significant in SY-M group (P<0.05); there was no significant difference in the above indices between SY-M group and JY-M group (P>0.05). 4) Compared with CON group, the villus height to crypt depth ratio of ileum in all FMT groups was increased, and the difference was significant in SY-M group (P<0.05); there was no significant difference in villus height to crypt depth ratio between SY-M group and JY-M group (P>0.05). In conclusion, the FMT of free range and captive Saba pigs can promote intestinal mucosa development, improve nutrient apparent digestibility, enhance lipid metabolism, reduce F/G, and thus improve growth performance of mice. Among them, the medium-dose fecal bacteria solution of free range Saba pigs has better transplantation effect.

Cite this article

CHENG Yandong , HU Tiannian , YIN Huajuan , CHA Guanghui , WANG Pu , YANG Kai , YE Pengfei , PAN Qiong , LIN Qiuye , CAO Zhenhui . Effects of Fecal Microbiota Transplantation of Free Range and Captive Saba Pigs on Growth Performance, Nutrient Apparent Digestibility and Intestinal Morphology in Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 8129 -8137 . DOI: 10.3969/j.issn.1006-267x.2022.12.059

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