1 材料与方法
1.1 试验设计
表1 试验饲粮组成及营养水平(风干基础)Table 1 Composition and nutrient levels of experimental diets (air-dry basis) % |
| 项目Items | A料Feed A | B料Feed B |
|---|---|---|
| 原料Ingredients | ||
| 玉米Corn | 43.24 | 33.05 |
| 膨化玉米Extruded corn | 15.00 | 15.00 |
| 小麦Wheat | 5.00 | 10.00 |
| 豆粕Soybean meal | 6.90 | 5.80 |
| 膨化大豆Extruded soybean | 9.00 | 11.00 |
| 发酵豆粕Fermented soybean meal | 7.50 | 7.50 |
| 鱼粉Fish meal | 2.50 | 3.00 |
| 乳清粉Dried whey | 6.00 | 6.00 |
| 大豆油Soybean oil | 2.00 | |
| 脂肪粉Fatty powder | 2.30 | |
| 石粉Limestone | 0.89 | 0.89 |
| 磷酸氢钙CaHPO4 | 0.63 | 0.62 |
| 食盐NaCl | 0.35 | 0.35 |
| L-赖氨酸盐酸盐L-Lys·HCl | 0.49 | 0.48 |
| DL-蛋氨酸DL-Met | 0.15 | 0.15 |
| L-苏氨酸L-Thr | 0.23 | 0.24 |
| L-色氨酸L-Trp | 0.04 | 0.04 |
| 氯化胆碱Choline chloride | 0.10 | 0.10 |
| 二氧化钛TiO2 | 0.40 | 0.40 |
| 预混料Premix1) | 1.58 | 1.58 |
| 合计Total | 100.00 | 100.00 |
| 营养水平Nutrient levels2) | ||
| 粗蛋白质CP | 17.80 | 17.84 |
| 消化能DE/(MJ/kg) | 14.43 | 15.27 |
| 净能NE/(MJ/kg) | 10.40 | 11.10 |
| 标准回肠可消化赖氨酸SID Lys | 1.14 | 1.14 |
| 标准回肠可消化苏氨酸SID Thr | 0.74 | 0.74 |
| 标准回肠可消化蛋氨酸+半胱氨酸SID Met+Cys | 0.65 | 0.65 |
| 标准回肠可消化色氨酸SID Trp | 0.21 | 0.21 |
| 钙Ca | 0.69 | 0.70 |
| 总磷TP | 0.52 | 0.51 |
| 标准全肠道可消化磷STTD P | 0.33 | 0.33 |
| 粗脂肪EE | 3.99 | 7.22 |
| 酸性洗涤纤维ADF | 3.17 | 3.12 |
| 中性洗涤纤维NDF | 7.91 | 7.63 |
| 干物质DM | 89.36 | 89.64 |
1)预混料为每千克饲粮提供The premix provided the following per kg of diets:VA 4 000 IU,VB1 10 mg,VB2 20 mg,VB5 75 mg,VB6 12.5 mg,VB12 125 μg,VD3 800 IU,VE 40 IU,VK3 2 mg,生物素 biotin 0.50 mg,叶酸 folic acid 2.5 mg,D-泛酸 D-pantothenic acid 75 mg,烟酸 nicotinic acid 100 mg,Cu (as copper sulfate) 10 mg,Fe (as ferrous sulfate) 100 mg,Mn (as manganese sulfate) 20 mg,Zn (as zinc sulfate) 105 mg,I (as potassium iodide) 0.20 mg,Se (as sodium selenite) 0.30 mg。2)粗蛋白质、粗脂肪、酸性洗涤纤维、中性洗涤纤维、钙、总磷、干物质为实测值,其余为计算值。CP, EE, ADF, NDF, Ca, TP, DM were measured values, while the others were calculated values. |
1.2 饲养管理
1.3 样品采集与测定
1.3.1 生长性能
1.3.2 仔猪腹泻率
1.3.3 养分表观消化率
1.3.4 血液生化指标
1.4 统计分析
2 结果
2.1 饲粮净能水平对广东小耳花猪生长性能的影响
表2 饲粮净能水平对广东小耳花猪生长性能的影响Table 2 Effects of dietary NE level on growth performance of Guangdong small-ear spotted piglets |
| 项目 Items | 饲粮净能水平Dietary NE levels/(MJ/kg) | SEM | P值P-value | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 10.40 | 10.58 | 10.75 | 10.93 | 11.10 | 方差分析 ANOVA | 线性 Linear | 二次 Quadratic | ||
| 32~46日龄32 to 46 days of age | |||||||||
| 初重Initial BW/kg | 4.60 | 4.60 | 4.60 | 4.60 | 4.60 | 0.003 | 0.999 | 0.935 | 0.936 |
| 末重Final BW/kg | 7.99 | 7.94 | 7.87 | 7.89 | 7.84 | 0.105 | 0.995 | 0.664 | 0.664 |
| 平均日增重ADG/g | 241.84 | 238.54 | 233.62 | 237.01 | 231.68 | 7.383 | 0.995 | 0.683 | 0.684 |
| 平均日采食量ADFI/g | 305.92 | 309.40 | 315.03 | 301.49 | 305.46 | 10.213 | 0.996 | 0.904 | 0.902 |
| 料重比F/G | 1.26 | 1.30 | 1.35 | 1.27 | 1.32 | 0.014 | 0.217 | 0.355 | 0.355 |
| 腹泻率Diarrhea rate/% | 8.12 | 6.77 | 5.84 | 7.99 | 5.42 | 0.782 | 0.770 | 0.462 | 0.462 |
| 47~60日龄47 to 60 days of age | |||||||||
| 末重Final BW/kg | 13.12 | 13.29 | 13.18 | 13.26 | 13.17 | 0.209 | 0.999 | 0.966 | 0.964 |
| 平均日增重ADG/g | 366.74 | 379.08 | 376.22 | 382.46 | 380.17 | 9.199 | 0.989 | 0.648 | 0.648 |
| 平均日采食量ADFI/g | 765.36 | 758.65 | 754.95 | 779.47 | 756.76 | 19.425 | 0.996 | 0.978 | 0.978 |
| 料重比F/G | 2.09 | 2.00 | 2.02 | 2.04 | 2.00 | 0.031 | 0.864 | 0.522 | 0.520 |
| 腹泻率Diarrhea rate/% | 2.06 | 0.78 | 1.58 | 1.11 | 0.46 | 0.367 | 0.186 | 0.128 | 0.127 |
| 32~60日龄32 to 60 days of age | |||||||||
| 平均日增重ADG/g | 304.29 | 309.84 | 306.02 | 309.86 | 305.92 | 7.420 | 0.999 | 0.949 | 0.949 |
| 平均日采食量ADFI/g | 535.64 | 534.03 | 534.99 | 540.48 | 531.11 | 14.301 | 0.999 | 0.980 | 0.981 |
| 料重比F/G | 1.76 | 1.72 | 1.75 | 1.74 | 1.74 | 0.015 | 0.944 | 0.818 | 0.918 |
| 腹泻率Diarrhea rate/% | 5.09 | 3.77 | 3.71 | 4.55 | 2.94 | 0.465 | 0.469 | 0.173 | 0.173 |
同行数据肩标不同小写字母表示差异显著(P<0.05),相同或无字母表示差异不显著(P>0.05)。下表同。 | |
In the same row, values with different small letter superscripts mean significant difference (P<0.05), while with the same or no letter superscripts mean no significant difference (P>0.05). The same as below. |
2.2 饲粮净能水平对广东小耳花猪养分表观消化率的影响
表3 饲粮净能水平对广东小耳花猪养分表观消化率的影响Table 3 Effects of dietary NE levels on nutrient apparent digestibility of Guangdong small-ear spotted piglets % |
| 项目 Items | 饲粮净能水平Dietary NE levels/(MJ/kg) | SEM | P值P-value | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 10.40 | 10.58 | 10.75 | 10.93 | 11.10 | 方差分析 ANOVA | 线性 Linear | 二次 Quadratic | ||
| 干物质DM | 79.03 | 81.20 | 82.53 | 78.11 | 80.04 | 0.587 | 0.124 | 0.373 | 0.170 |
| 总能GE | 82.77 | 84.62 | 85.89 | 82.55 | 84.36 | 1.053 | 0.147 | 0.096 | 0.096 |
| 粗蛋白质CP | 73.75 | 75.86 | 76.32 | 72.44 | 75.13 | 0.793 | 0.547 | 0.133 | 0.308 |
| 粗脂肪EE | 59.35c | 64.97bc | 71.24ab | 69.33ab | 73.11a | 1.399 | 0.006 | 0.001 | 0.001 |
| 粗灰分Ash | 29.16 | 29.31 | 31.44 | 27.55 | 26.38 | 1.450 | 0.854 | 0.530 | 0.639 |
2.3 饲粮净能水平对广东小耳花猪血清生化指标的影响
表4 饲粮净能水平对广东小耳花猪血清生化指标的影响Table 4 Effects of dietary NE levels on serum biochemical indexes of Guangdong small-ear spotted piglets |
| 项目 Items | 饲粮净能水平Dietary NE levels/(MJ/kg) | SEM | P值P-value | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 10.40 | 10.58 | 10.75 | 10.93 | 11.10 | 方差分析 ANOVA | 线性 Linear | 二次 Quadratic | ||
| 白蛋白ALB/(g/L) | 36.60 | 37.10 | 36.56 | 38.70 | 39.97 | 0.742 | 0.364 | 0.046 | 0.046 |
| 总蛋白TP/(g/L) | 60.19 | 61.42 | 58.55 | 63.01 | 62.96 | 0.684 | 0.180 | 0.142 | 0.140 |
| 肌酐CRE/(μmol/L) | 61.67 | 63.41 | 63.45 | 71.64 | 66.35 | 1.541 | 0.280 | 0.109 | 0.109 |
| 尿素UN/(mmol/L) | 3.40 | 3.19 | 2.92 | 4.35 | 3.19 | 0.212 | 0.264 | 0.630 | 0.630 |
| 葡萄糖GLU/(mmol/L) | 6.62 | 6.58 | 5.66 | 5.20 | 5.35 | 0.248 | 0.202 | 0.022 | 0.024 |
| 甘油三酯TG/(mmol/L) | 0.46 | 0.47 | 0.46 | 0.50 | 0.42 | 0.254 | 0.865 | 0.794 | 0.789 |
| 总胆固醇 TCHO/(mmol/L) | 2.45 | 2.41 | 2.37 | 2.65 | 2.18 | 0.082 | 0.510 | 0.592 | 0.596 |
| 高密度脂蛋白胆固醇 HDL-C/(mmol/L) | 0.83 | 0.73 | 0.83 | 0.93 | 0.75 | 0.032 | 0.121 | 0.820 | 0.820 |
| 低密度脂蛋白胆固醇 LDL-C/(mmol/L) | 1.22 | 1.33 | 1.12 | 1.30 | 1.12 | 0.052 | 0.539 | 0.504 | 0.501 |
| 谷丙转氨酶ALT/(U/L) | 62.70 | 58.04 | 55.51 | 58.38 | 56.31 | 2.508 | 0.925 | 0.495 | 0.495 |
| 谷草转氨酶AST/(U/L) | 53.72b | 81.99a | 45.89b | 66.82b | 57.07b | 3.727 | 0.014 | 0.755 | 0.751 |
| 碱性磷酸酶ALP/(U/L) | 279.18 | 279.83 | 314.48 | 340.76 | 340.57 | 19.010 | 0.748 | 0.176 | 0.176 |
| 总胆红素 TBIL/(μmol/L) | 21.97 | 24.33 | 20.18 | 25.98 | 25.74 | 1.484 | 0.713 | 0.391 | 0.390 |
2.4 饲粮净能水平对广东小耳花猪血浆抗氧化指标的影响
表5 饲粮净能水平对广东小耳花猪血浆抗氧化指标的影响Table 5 Effects of dietary NE levels on plasma antioxidant indexes of Guangdong small-ear spotted piglets |
| 项目 Items | 饲粮净能水平Dietary NE levels/(MJ/kg) | SEM | P值P-value | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 10.40 | 10.58 | 10.75 | 10.93 | 11.10 | 方差分析 ANOVA | 线性 Linear | 二次 Quadratic | ||
| 总抗氧化能力 T-AOC/(mmol/L) | 0.58 | 0.53 | 0.55 | 0.58 | 0.51 | 0.022 | 0.840 | 0.587 | 0.586 |
| 总超氧化物歧化酶 T-SOD/(U/mL) | 22.69 | 26.29 | 25.41 | 25.17 | 24.59 | 0.548 | 0.321 | 0.501 | 0.501 |
| 谷胱甘肽过氧化物酶 GSH-Px/(U/mL) | 1 502 | 1 418 | 1 478 | 1 484 | 1 594 | 34.5 | 0.636 | 0.315 | 0.310 |
| 过氧化氢酶 CAT/(U/mL) | 1.46 | 3.14 | 3.14 | 4.03 | 4.12 | 0.372 | 0.154 | 0.016 | 0.016 |
| 丙二醛 MDA/(nmol/mL) | 6.36 | 8.73 | 8.48 | 8.92 | 8.22 | 0.371 | 0.187 | 0.138 | 0.138 |
2.5 饲粮净能水平对广东小耳花猪血浆免疫指标的影响
表6 饲粮净能水平对广东小耳花猪血浆免疫指标的影响Table 6 Effects of dietary NE levels on plasma immune indexes of Guangdong small-ear spotted piglets |
| 项目 Items | 饲粮净能水平Dietary NE levels/(MJ/kg) | SEM | P值P-value | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 10.40 | 10.58 | 10.75 | 10.93 | 11.10 | 方差分析 ANOVA | 线性 Linear | 二次 Quadratic | ||
| 白细胞介素-1β IL-1β/(ng/L) | 5.57 | 5.69 | 5.52 | 5.27 | 5.36 | 0.128 | 0.866 | 0.356 | 0.356 |
| 肿瘤坏死因子-α TNF-α/(pg/mL) | 53.27 | 54.55 | 51.19 | 53.11 | 49.84 | 1.704 | 0.929 | 0.500 | 0.499 |
| 免疫球蛋白A IgA/(μg/mL) | 29.98 | 27.41 | 27.87 | 27.06 | 27.36 | 0.461 | 0.271 | 0.086 | 0.086 |
| 免疫球蛋白G IgG/(μg/mL) | 437.07 | 404.89 | 433.33 | 435.53 | 440.52 | 6.000 | 0.356 | 0.415 | 0.410 |
| 免疫球蛋白M IgM/(μg/mL) | 40.09 | 39.92 | 40.47 | 39.54 | 40.95 | 0.487 | 0.926 | 0.704 | 0.702 |
