Chinese Journal of Animal Nutrition >

2025 , Vol. 37 >Issue 12: 8585 - 8593

DOI: https://doi.org/10.12418/CJAN2025.699

RESEARCH PAPER

Effects of Lactobacillus reuteri Postbiotics on Growth Performance,Nutrient Apparent Digestibility, and Serum Immune,Antioxidant and Biochemical Indexes of Growing Female Minks

  • JIANG Ziyi ,
  • CHEN Jian ,
  • KONG Lingpeng ,
  • ZHANG Huanle ,
  • WANG Guang ,
  • WANG Lihua , *
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  • College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China
* professor, E-mail:

Received date: 2025-05-23

  Online published: 2025-12-13

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Abstract

This study was conducted to investigate the effects of adding Lactobacillus reuteri postbiotics (LRP) to the basal diet on the growth performance, nutrient apparent digestibility, and serum immune, antioxidant and biochemical indexes of growing female minks. Eighty-eight healthy 12-week-old red-eyed white female minks with similar body weight were randomly divided into four groups, with 11 replicates per group and two minks per replicate. The LRP addition levels in diets were 0 (control group), 0.05% (0.05%LRP group), 0.10% (0.10%LRP group), and 0.15% (0.15%LRP group), respectively. The pre-experimental period lasted for 1 week, and the formal experimental period lasted for 8 weeks. The digestion trial was conducted in the 7th week of the formal experiment. The results showed as follows: 1) compared with the control group, the body weight at the end of weeks 4 and 8, as well as the average daily gain (ADG) during weeks 1 to 4, 5 to 8, and 1 to 8, in both the 0.10%LRP group and 0.15%LRP group were significantly increased (P<0.05); furthermore, the feed-to-gain ratio (F/G) during weeks 1 to 4 and 1 to 8 in the 0.10%LRP group, and during weeks 1 to 4 in the 0.15%LRP group was significantly decreased (P<0.05). 2) Compared with the control group, the 0.10%LRP group and 0.15%LRP group showed a significant increase in the apparent digestibility of dry matter and crude protein (P<0.05). 3) Compared with the control group, 0.10%LRP group exhibited a significant increase in serum immunoglobulin A (IgA) and immunoglobulin M (IgM) contents (P<0.05), while the 0.15%LRP group showed significantly higher serum IgA and complement 4 (C4) contents (P<0.05). 4) Compared with the control group, the content of serum malondialdehyde in the 0.05%LRP group and 0.10%LRP group was significantly decreased (P<0.05), and the serum total antioxidant capacity in the 0.10%LRP group was significantly increased (P<0.05). 5) Compared with the control group, the 0.10%LRP group exhibited a significant elevation in serum albumin, globulin and total protein contents (P<0.05), while the 0.15%LRP group showed a significant increase in serum globulin and total protein contents (P<0.05). In summary, Lactobacillus reuteri postbiotics has probiotic effects, it can enhance the dietary nutrient digestion, improve the immune and antioxidant capacities of growing female minks, thereby enhancing their growth performance. The opium addition level of Lactobacillus reuteri postbiotics is 0.10%.

Key words： Lactobacillus reuteri postbiotics; female minks; growth performance; nutrient apparent digestibility; immune; antioxidant

Cite this article

JIANG Ziyi , CHEN Jian , KONG Lingpeng , ZHANG Huanle , WANG Guang , WANG Lihua . Effects of Lactobacillus reuteri Postbiotics on Growth Performance,Nutrient Apparent Digestibility, and Serum Immune,Antioxidant and Biochemical Indexes of Growing Female Minks[J]. Chinese Journal of Animal Nutrition, 2025 , 37(12) : 8585 -8593 . DOI: 10.12418/CJAN2025.699

罗伊氏乳杆菌(Lactobacillus reuteri)是一种专性异型发酵乳酸杆菌[1],具有抑制有害菌[2]、提高机体抗氧化能力及免疫功能[3]、改善肠道健康[4]等益生作用,在畜牧生产中广泛应用。研究发现,益生菌在灭活状态下也能产生与活菌相似甚至增强的效果[5]。国际益生菌和益生元科学协会(ISAPP)将能为宿主带来益处的无生命的微生物和/或其成分制剂定义为后生元[6]。Sun等[7]研究发现,在仔猪基础饲粮中添加罗伊氏乳杆菌益生元能够调节仔猪肠道菌群结构,提高仔猪抗氧化能力。然而,目前关于罗伊氏乳杆菌后生元在水貂上的应用研究较少。鉴于此,本试验在母貂饲粮中添加不同水平的罗伊氏乳杆菌后生元,研究其对母貂生长性能、养分表观消化率及血清抗氧化、免疫和生化指标的影响,以确定母貂饲粮中罗伊氏乳杆菌后生元的适宜添加水平,为罗伊氏乳杆菌后生元在水貂生产中的应用提供科学依据。

1 材料与方法

1.1 试验材料

试验所用罗伊氏乳杆菌是从健康水貂肠道内容物中分离纯化得到,现保存于中国普通微生物菌种保藏管理中心,编号为No.30786。将分离纯化的罗伊氏乳杆菌接种于MRS液体培养基中,37 ℃培养24 h,且实测活菌数大于109 CFU/mL,经121 ℃高温处理15 min,制备成罗伊氏乳杆菌后生元。

1.2 试验设计

试验采用单因素试验设计,选取88只12周龄健康且体重相近的白色母貂,随机分为4组,每组11个重复,每个重复2只。4组分别饲喂在基础饲粮中添加0(对照组)、0.05%(0.05%LRP组)、0.10%(0.10%LRP组)、0.15%(0.15%LRP组)的罗伊氏乳杆菌后生元的试验饲粮。基础饲粮组成及营养水平见表1。预试期1周,正试期8周。动物试验经青岛农业大学动物科技学院实验动物伦理审查委员会批准(批准号:DKY20240514-3)。
表1 基础饲粮组成及营养水平

Table 1 Composition and nutrient levels of basal diets %

项目
Items		 1~4周
1 to 4
weeks		 5~8周
5 to 8
weeks
原料(饲喂基础) Ingredients (as-fed basis)
毛蛋Unhatched fertilized egg 23 23
鸡骨架Chicken frames 15 15
鸡头Chicken head 15 16
海杂鱼Mixed saltwater fish 12 12
鳕鱼排Cod steak 10 10
鸡肝Chicken liver 8 8
膨化玉米Extruded corn 5 6
安康鱼头Monkfish head 5 4
膨化豆粕Extruded soybean meal 3 3
豆油Soybean oil 2 2
猪血粉Pork blood meal 1
预混料Premix1) 1 1
合计Total 100 100
营养水平(风干基础) Nutrient levels (air-dry basis)2)
代谢能ME/(MJ/kg) 17.96 17.99
粗蛋白质CP 36.69 32.96
粗脂肪EE 24.35 25.40
粗灰分Ash 11.92 12.59
钙Ca 3.97 3.85
磷P 1.03 1.05

1)预混料为每千克饲粮提供 The premix provided the following per kg of diets:VA 6 000 IU,VD3 540 IU,VE 37.5 mg,VK3 0.72 mg,VB1 11.4 mg,VB2 7.2 mg,VB6 3.6 mg,VB12 0.019 5 mg,VC 30 mg,生物素 biotin 0.12 mg,叶酸 folic acid 0.6 mg,烟酸 nicotinic acid 14.7 mg,泛酸 pantothenic acid 5.7 mg,氯化胆碱 choline chloride 150 mg,Fe 30 mg,Cu 12.6 mg,Mn 15 mg,Zn 30 mg。

2)代谢能为计算值,其他为实测值。代谢能根据《水貂、狐、貉营养指南》(T/SDAA 0095—2024)中给出的计算公式计算:代谢能(MJ/kg)=[0.85×粗蛋白质(%)×4.5+0.90×粗脂肪(%)×9.5+0.75×无氮浸出物(%)×4.0]×4.18/100;无氮浸出物(%)=100-粗蛋白质(%)-粗脂肪(%)-粗灰分(%)。ME was a calculated value, while the others were measured values. ME was calculated using the formula recommended by Nutritional Guidelines for Mink, Fox and Raccoon Dog (T/SDAA 0095—2024): ME (MJ/kg)=[0.85×CP(%)×4.5+0.90×EE(%)×9.5+0.75×NFE(%)×4.0]×4.18/100; NFE(%)=100-CP(%)-EE(%)-Ash(%).

1.3 饲养管理

饲养试验于2024年7—9月在烟台市海阳某水貂养殖场进行。试验前,试验水貂均已完成疫苗的接种和褪黑激素的埋植。貂舍为双列式棚舍,试验期间的平均气温为(27.64±3.25) ℃,相对湿度为(69.50±8.13)%。水貂采用笼养,每笼饲养2只。试验期内,水貂每天饲喂2次,保证自由饮水。

1.4 测定指标及方法

1.4.1 生长性能

在正试期第4周和第8周末(试验结束时)对水貂进行空腹称重,计算平均日增重;每周选3 d记录水貂的给料量和剩料量,计算平均日采食量;根据平均日采食量及平均日增重计算料重比。

1.4.2 养分表观消化率

试验进行到第7周时,每组随机选取6笼水貂(n=6)进行消化试验。消化试验采用内源指示剂法,连续采集3 d新鲜的水貂粪便,同时采集每组的饲粮样品。将混匀的粪便和饲粮样品于65 ℃烘干,制成风干样后粉碎过40目筛,用于测定干物质(GB/T 6435—2014)、粗蛋白质(GB/T 6432—2018)、粗脂肪(GB/T 6433—2006)、粗灰分(GB/T 6438—2007)、钙(GB/T 6436—2018)、磷(GB/T 6437—2018)和盐酸不溶灰分(GB/T 23742—2009)含量,并计算养分表观消化率[8]。

1.4.3 血清免疫、抗氧化及生化指标

饲养试验结束时,每组随机选取8只健康母貂(n=8)进行心脏采血10 mL,2 025×g离心10 min制备出血清,分装后-80 ℃保存。血清免疫指标:采用酶联免疫吸附试验(ELISA)法测定免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、免疫球蛋白M(IgM)及补体4(C4)含量。血清抗氧化指标:采用比色法测定谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力(T-AOC),采用羟胺法测定总超氧化物歧化酶(T-SOD)活性,采用硫代巴比妥酸(TBA)法测定丙二醛(MDA)含量。血清生化指标:采用考马斯亮蓝法测定总蛋白(TP)含量,采用微板法测定白蛋白(ALB)含量、碱性磷酸酶(AKP)活性,采用ELISA法测定GLB含量,采用脲酶法测定尿素氮(UN)的含量。所用试剂盒均购自南京建成生物工程研究所。

1.5 数据统计分析

试验数据采用SPSS 25.0软件的ANOVA过程进行单因素方差分析,采用Duncan法进行比较组间多重比较,试验结果以平均值±标准误表示,P<0.05表示差异显著。

2 结果

2.1 罗伊氏乳杆菌后生元对生长期母貂生长性能的影响

由表2可知,罗伊氏乳杆菌后生元添加水平显著影响生长期母貂在试验第4周和第8周末体重以及第1~4周、第5~8周、第1~8周平均日增重和第1~4周和第1~8周料重比(P<0.05),对各阶段的平均日采食量无显著影响(P>0.05)。与对照组相比,0.10%LRP组和0.15%LRP组第4周和第8周末体重以及第1~4周、第5~8周、第1~8周平均日增重显著提高(P<0.05);0.10%LRP组第1~4周和第1~8周料重比显著降低(P<0.05),0.15%LRP组第1~4周料重比显著降低(P<0.05)。
表2 罗伊氏乳杆菌后生元对生长期母貂生长性能的影响

Table 2 Effects of Lactobacillus reuteri postbiotics on growth performance of growing female minks

项目
Items		 组别Groups P值
P-value
对照Control 0.05%LRP 0.10%LRP 0.15%LRP
体重BW/g
初始Initial 950.23±12.54 953.18±15.63 948.41±7.81 945.68±15.53 0.982
第4周末The end of week 4 1 205.91±15.19b 1 241.36±11.18ab 1 259.09±8.86a 1 247.73±16.03a 0.040
第8周末The end of week 8 1 340.91±18.15b 1 389.55±17.18ab 1 423.18±20.20a 1 400.00±17.54a 0.022
平均日增重ADG/g
第1~4周Weeks 1 to 4 9.36±0.43b 10.29±0.39ab 11.10±0.48a 10.79±0.41a 0.037
第5~8周Weeks 5 to 8 4.43±0.36b 5.50±0.32ab 6.05±0.53a 5.78±0.42a 0.047
第1~8周Weeks 1 to 8 6.98±0.22b 7.79±0.28ab 8.48±0.41a 8.11±0.31a 0.011
平均日采食量ADFI/g
第1~4周Weeks 1 to 4 205.69±4.94 213.22±4.18 206.18±1.98 207.50±4.17 0.522
第5~8周Weeks 5 to 8 195.63±5.72 207.88±7.53 200.44±6.68 206.15±9.75 0.654
第1~8周Weeks 1 to 8 200.66±4.85 210.55±4.82 203.31±3.97 206.83±6.04 0.531
料重比F/G
第1~4周Weeks 1 to 4 22.34±0.91a 21.06±1.01a 18.99±0.95b 19.54±0.57b 0.042
第5~8周Weeks 5 to 8 46.71±3.37 40.87±2.79 34.44±3.02 38.73±2.72 0.050
第1~8周Weeks 1 to 8 28.92±0.76a 27.39±1.21ab 24.50±1.18b 25.86±1.19ab 0.041

同行数据肩标无字母或相同字母表示差异不显著(P>0.05),不同小写字母表示差异显著(P<0.05)。下表同。

In the same row, values with no letter or the same letter superscripts indicated no significant difference (P>0.05), while with different lowercase letter superscripts indicated significant difference (P<0.05). The same as below.

2.2 罗伊氏乳杆菌后生元对生长期母貂养分表观消化率的影响

由表3可知,罗伊氏乳杆菌后添加水平显著影响生长期母貂的干物质和粗蛋白质表观消化率(P<0.05),对粗脂肪、粗灰分、钙及磷表观消化率无显著影响(P>0.05)。与对照组相比,在饲粮中添加0.10%和0.15%罗伊氏乳杆菌后生元可显著提高母貂的干物质和粗蛋白质表观消化率(P<0.05)。
表3 罗伊氏乳杆菌后生元对生长期母貂养分表观消化率的影响

Table 3 Effects of Lactobacillus reuteri postbiotics on nutrient apparent digestibility of growing female minks %

项目
Items		 组别Groups P值
P-value
对照Control 0.05%LRP 0.10%LRP 0.15%LRP
干物质DM 74.28±0.84b 75.98±0.88ab 77.98±0.86a 77.47±0.72a 0.022
粗蛋白质CP 85.54±0.53b 86.19±0.47ab 87.72±0.74a 88.82±0.57a 0.028
粗脂肪EE 97.46±0.17 97.76±0.23 97.79±0.18 98.00±0.18 0.284
粗灰分Ash 25.55±3.10 29.15±3.98 31.19±2.66 26.07±4.00 0.633
钙Ca 21.06±3.28 23.13±2.62 22.86±2.47 23.81±3.50 0.926
磷P 27.24±3.71 28.26±3.53 30.33±3.06 29.08±2.20 0.916

2.3 罗伊氏乳杆菌后生元对生长期母貂血清免疫指标的影响

由表4可知,罗伊氏乳杆菌后生元添加水平显著影响生长期母貂血清IgA、IgM及C4含量(P<0.05),对血清IgG含量无显著影响(P>0.05)。与对照组相比,0.10%LRP组血清IgA和IgM含量、0.15%LRP组血清IgA和C4含量显著增加(P<0.05)。
表4 罗伊氏乳杆菌后生元对生长期母貂血清免疫指标的影响

Table 4 Effects of Lactobacillus reuteri postbiotics on serum immune indexes of growing female minks

项目
Items		 组别Groups P值
P-value
对照Control 0.05%LRP 0.10%LRP 0.15%LRP
免疫球蛋白A IgA/(μg/mL) 271.81±10.51b 301.49±8.52ab 324.38±17.46a 311.50±11.03a 0.038
免疫球蛋白G IgG/(g/L) 22.79±0.87 23.86±1.02 25.87±1.10 25.11±0.84 0.143
免疫球蛋白M IgM/(μg/mL) 2 622.83±106.49c 2 838.09±97.12bc 3 473.84±147.97a 3 124.26±194.95ab 0.002
补体4 C4/(μg/mL) 201.82±7.69b 212.39±9.02b 216.29±9.71ab 242.08±10.72a 0.036

2.4 罗伊氏乳杆菌后生元对生长期母貂血清抗氧化指标的影响

由表5可知,罗伊氏乳杆菌后生元添加水平显著影响血清MDA含量和T-AOC(P<0.05),对血清T-SOD和GSH-Px活性无显著影响(P>0.05)。与对照组相比,0.05%LRP组和0.10%LRP组血清MDA含量均显著降低(P<0.05),0.10%LRP组血清T-AOC显著提高(P<0.05)。此外,0.10%LRP组血清T-AOC显著高于0.05%LRP组(P<0.05),与0.15%LRP组差异不显著(P>0.05)。
表5 罗伊氏乳杆菌后生元对生长期母貂血清抗氧化指标的影响

Table 5 Effects of Lactobacillus reuteri postbiotics on serum antioxidant indexes of growing female minks

项目
Items		 组别Groups P值
P-value
对照Control 0.05%LRP 0.10%LRP 0.15%LRP
总超氧化物歧化酶
T-SOD/(U/mL)		 156.97±1.56 160.06±5.87 160.56±2.28 160.25±1.95 0.865
丙二醛MDA/(nmol/mL) 14.09±1.34a 9.87±0.90b 9.11±0.99b 11.36±1.41ab 0.029
总抗氧化能力T-AOC/(U/mL) 10.85±0.88b 11.10±0.80b 13.95±0.70a 12.37±0.83ab 0.042
谷胱甘肽过氧化物酶
GSH-Px/(U/mL)		 3 146.03±83.54 3 188.33±109.93 3 255.90±71.83 3 081.86±93.14 0.589

2.5 罗伊氏乳杆菌后生元对生长期母貂血清生化指标的影响

由表6可知,罗伊氏乳杆菌后生元添加水平影响显著血清ALB、GLB及TP含量(P<0.05),对血清UN含量和AKP活性无显著影响(P>0.05)。与对照组相比,0.10%LRP组血清ALB、GLB及TP含量显著增加(P<0.05),0.15%LRP组血清GLB和TP含量显著增加(P<0.05)。
表6 罗伊氏乳杆菌后生元对生长期母貂血清生化指标的影响

Table 6 Effects of Lactobacillus reuteri postbiotics on serum biochemical indexes of growing female minks

项目
Items		 组别Groups P值
P-value
对照Control 0.05%LRP 0.10%LRP 0.15%LRP
白蛋白ALB/(g/L) 26.83±1.00b 27.87±1.06ab 30.51±0.51a 29.55±0.98ab 0.039
球蛋白GLB/(g/L) 32.05±1.03b 33.90±0.83ab 36.48±0.66a 35.21±1.43a 0.035
尿素氮UN/(mmol/L) 4.94±0.11 5.03±0.39 4.69±0.35 4.49±0.52 0.732
总蛋白TP/(g/L) 58.89±1.34b 61.77±1.68ab 66.98±2.32a 64.75±1.72a 0.022
碱性磷酸酶AKP/(金氏单位/dL) 13.65±1.36 15.44±1.10 15.02±1.30 17.25±1.57 0.325

3 讨论

3.1 罗伊氏乳杆菌后生元对生长期母貂生长性能的影响

Tsega等[9]研究表明,罗伊氏乳杆菌能够显著提高罗得岛红鸡的生长性能。刘春艳等[10]研究发现,在饲粮中添加罗伊氏乳杆菌可以提高断奶仔猪平均日增重,降低料重比。本试验结果显示,在饲粮中添加罗伊氏乳杆菌后生元能够改善生长期母貂的生长性能。这与Melese等[11]的研究结果一致,说明罗伊氏乳杆菌后生元具有与益生菌类似的益生作用。这可能是罗伊氏乳杆菌产生的活性物质可以抑制病原菌定植[12],维持了水貂肠道的正常功能,促进了对养分的消化吸收。

3.2 罗伊氏乳杆菌后生元对生长期母貂养分表观消化率的影响

养分消化率能够反映动物机体对营养物质消化吸收的能力。王卓等[13]研究表明,饲粮添加罗伊氏乳杆菌能够显著提高断奶驴驹的中性洗涤纤维和酸性洗涤纤维表观利用率。徐大海等[14]研究发现,在基础饲粮中添加后生元能够显著提高肉鸡的粗脂肪表观代谢率。Izuddin等[15]研究发现,在饲粮中添加后生元可显著提高断奶后羔羊的干物质及粗蛋白质表观消化率。Vicente等[16]研究表明,在饲粮中添加后生元能够提高过渡期奶牛干物质、粗蛋白质及中性洗涤纤维的表观消化率。本试验也发现,在饲粮中添加0.10%和0.15%的罗伊氏乳杆菌后生元可显著提高生长期母貂的干物质和粗蛋白质表观消化率。生长期母貂干物质及粗蛋白质表观消化率的提高可能由于罗伊氏乳杆菌产生的短链脂肪酸能够通过扩散穿过细胞膜,降低胃肠道pH[17],调节了肠道胰蛋白酶、淀粉酶、脂肪酶等消化酶活性[18],抑制了病原体的入侵[19],维持了肠道功能稳态,进而促进了母貂对饲粮中干物质及粗蛋白质的消化吸收。

3.3 罗伊氏乳杆菌后生元对生长期母貂血清免疫指标的影响

免疫球蛋白是大多存在于血清中的免疫活性球状蛋白,能与外来抗原发生反应[20],对动物机体具有保护作用。补体C3、C4是补体系统的重要成分,在机体免疫应答和预防感染中起着关键性作用[21]。李亚霖等[22]研究发现,在水貂饲粮中添加植物乳杆菌后生元能够显著提高血清IgG的含量。李元元等[23]研究表明,补饲牛源罗伊氏乳杆菌组小鼠的血清IgA、IgG、IgM含量显著增加。Zhang等[24]研究发现,罗伊氏乳杆菌可以提高猪血清IgM含量。本试验发现,饲粮中添加0.10%和0.15%罗伊氏乳杆菌后生元均可显著提高生长期母貂血清IgA含量,添加0.10%罗伊氏乳杆菌后生元还可显著提高血清IgM含量,添加0.15%罗伊氏乳杆菌后生元还可显著提高血清C4含量。这可能是由于罗伊氏乳杆菌细胞壁成分如脂磷壁酸等物质可以释放抗感染肽,诱导了免疫调节因子的释放[25],从而提高了免疫球蛋白及补体含量,进而增强了机体的免疫水平。此外,不同添加水平的罗伊氏乳杆菌后生元产生的免疫效果不同,可能是因为不同添加水平的罗伊氏乳杆菌后生元中的不同活性成分激活了水貂体内的不同免疫通路或免疫细胞类型,从而产生差异化的免疫应答效果。

3.4 罗伊氏乳杆菌后生元对生长期母貂血清抗氧化指标的影响

MDA可损害细胞的物理活性并改变生物膜功能,通常被采用作为氧化损伤的生物标志物[26-27]。T-AOC是反映机体抗氧化能力的重要指标。有研究表明,在饲粮中添加罗伊氏乳杆菌可以提高仔猪血浆T-AOC及GSH-Px、超氧化物歧化酶(SOD)活性,降低血浆MDA含量[28]。殷成港等[29]研究发现,在断奶仔猪饲粮中添加后生元后,血浆SOD及GSH-Px活性提高,血浆MDA含量显著降低。本试验发现,饲粮中添加0.05%和0.10%的罗伊氏乳杆菌后生元可显著降低生长期母貂血清MDA含量,添加0.10%的罗伊氏乳杆菌后生元还可显著提高血清T-AOC,说明饲粮中添加罗伊氏乳杆菌后生元可提高水貂的抗氧化能力,与上述研究结果一致。这可能是因为罗伊氏乳杆菌后生元中的胞外多糖(EPS)存在羟基(-OH),可以螯合Fe2+、Cu2+等金属离子,增强了机体对超氧阴离子和羟基自由基的清除能力,减少了体内活性氧的产生,进而减少了机体的氧化应激[30-31]。此外,本试验结果还显示,0.10%LRP组母貂的血清T-AOC显著高于0.05%LRP组,与0.15%LRP组差异不显著,此研究结果与Tian等[32]的研究结果一致,说明0.10%的罗伊氏乳杆菌后生元能发挥较好的抗氧化作用。

3.5 罗伊氏乳杆菌后生元对生长期母貂血清生化指标的影响

血清ALB可以维持动物机体正常的营养代谢水平,GLB可以维持动物机体的免疫力[33],TP可以反映动物对饲粮中蛋白质的利用率,是评价蛋白质代谢的重要指标[34]。黄炜乾等[35]研究表明,饲粮中添加低剂量的后生元能够提高清远麻鸡血清TP含量。Ha等[36]和Kang等[37]的研究证实,饲粮中添加罗伊氏乳杆菌能够调节断奶仔猪血清生化指标。本试验也发现,饲粮中添加0.10%的罗伊氏乳杆菌后生元能够显著提高生长期母貂血清ALB、GLB、TP含量,这可能是罗伊氏乳杆菌的代谢产物可调节蛋白质代谢,增强了体内蛋白质沉积[38]。

4 结论

罗伊氏乳杆菌后生元具有益生作用,能够促进生长期母貂对养分的消化,增强机体免疫力和抗氧化能力,进而改善生长性能。结合各项测定指标,建议罗伊氏乳杆菌后生元添加水平为0.10%。

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