1 材料与方法
1.1 试验材料
表1 水溶性玉米蛋白粉与常规玉米蛋白粉、豆粕的粗蛋白质含量和氨基酸组成对比Table 1 Comparison of crude protein content and amino acid composition between water-soluble corn gluten meal and conventional corn gluten meal & soybean meal |
| 项目 Items | 水溶性玉米蛋白粉 Water-soluble corn gluten meal | 常规玉米蛋白粉 Conventional corn gluten meal | 豆粕 Soybean meal |
|---|---|---|---|
| 粗蛋白质Crude protein | 62.50 | 58.25 | 47.73 |
| 赖氨酸Lysine | 2.33 | 0.93 | 2.96 |
| 蛋氨酸Methionine | 0.73 | 1.21 | 0.66 |
| 苏氨酸Threonine | 2.43 | 1.81 | 1.86 |
| 色氨酸Tryptophan | 0.14 | 0.27 | 0.66 |
| 半胱氨酸Cystine | 2.51 | 1.01 | 0.70 |
| 苯丙氨酸Phenylalanine | 1.80 | 3.25 | 2.40 |
| 亮氨酸Leucine | 3.48 | 9.82 | 3.62 |
| 异亮氨酸Isoleucine | 1.51 | 2.23 | 2.14 |
| 缬氨酸Valine | 2.49 | 2.42 | 2.06 |
| 谷氨酸Glutamic acid | 11.77 | 11.20 | 7.56 |
| 天冬氨酸Aspartic acid | 3.83 | 2.97 | 4.84 |
| 丝氨酸Serine | 2.78 | 2.29 | 2.11 |
| 甘氨酸Glycine | 3.51 | 1.28 | 1.89 |
| 丙氨酸Alanine | 3.84 | 4.33 | 1.89 |
| 酪氨酸Tyrosine | 1.42 | 2.86 | 1.50 |
1.2 试验设计
表2 饲粮组成及营养水平(风干基础)Table 2 Composition and nutrient levels of diets (air-dry basis) |
| 项目 Items | 对照组 Control group | 试验Ⅰ组 Experimental group Ⅰ | 试验Ⅱ组 Experimental group Ⅱ |
|---|---|---|---|
| 原料Ingredients | |||
| 玉米Corn | 38.67 | 40.25 | 41.93 |
| 膨化玉米Extruded corn | 15.00 | 15.00 | 15.00 |
| 发酵豆粕Fermented soybean meal | 3.50 | 3.40 | 3.40 |
| 膨化全脂大豆Extruded full-fat soybean | 10.00 | 10.00 | 10.00 |
| 乳清粉Whey powder | 10.00 | 10.00 | 10.00 |
| 豆粕Soybean meal | 10.00 | 5.00 | |
| 鱼粉Fish meal | 4.00 | 4.00 | 4.00 |
| 水溶性玉米蛋白粉Water-soluble corn gluten meal | 3.25 | 6.50 | |
| 大豆油Soybean oil | 1.40 | 1.47 | 1.47 |
| 蔗糖Sucrose | 2.00 | 2.00 | 2.00 |
| 石粉Limestone | 0.98 | 0.97 | 0.97 |
| 磷酸氢钙CaHPO4 | 0.45 | 0.60 | 0.65 |
| L-赖氨酸盐酸盐L-Lys·HCl (≥98.5%) | 0.72 | 0.78 | 0.82 |
| DL-蛋氨酸DL-Met (≥99.0%) | 0.29 | 0.25 | 0.22 |
| L-苏氨酸L-Thr (≥98.5%) | 0.29 | 0.30 | 0.29 |
| L-色氨酸L-Trp (≥98.0%) | 0.05 | 0.08 | 0.10 |
| 食盐NaCl | 0.30 | 0.30 | 0.30 |
| 碳酸氢钠NaHCO3 | 0.20 | 0.20 | 0.20 |
| 氯化胆碱Choline chloride (50%) | 0.15 | 0.15 | 0.15 |
| 预混料Premix1) | 2.00 | 2.00 | 2.00 |
| 合计Total | 100.00 | 100.00 | 100.00 |
| 营养水平Nutrient levels2) | |||
| 消化能DE/(MJ/kg) | 14.94 | 14.94 | 14.94 |
| 总能GE/(MJ/kg) | 16.72 | 16.76 | 16.50 |
| 粗蛋白质CP | 17.80 | 17.85 | 17.82 |
| 赖氨酸Lys | 1.50 | 1.49 | 1.52 |
| 苏氨酸Thr | 0.95 | 0.94 | 0.94 |
| 蛋氨酸+半胱氨酸Met+Cys | 0.89 | 0.88 | 0.89 |
| 色氨酸Trp | 0.24 | 0.25 | 0.25 |
| 钙Ca | 0.71 | 0.73 | 0.74 |
| 总磷TP | 0.50 | 0.50 | 0.51 |
| 标准全消化道可消化磷STTD P | 0.33 | 0.33 | 0.33 |
1)预混料为每千克饲粮提供 The premix provided the following per kg of diets:VA 4 400 IU,VD3 440 IU,VE 30 IU,VK 1 mg,VB12 40 μg,VB1 3 mg,VB2 10 mg,烟酸 nicotinic acid 40 mg,D-泛酸 D-pantothenic acid 15 mg,叶酸 folic acid 1 mg,VB6 8 mg,生物素 biotin 0.08 mg,Fe (FeSO4·H2O) 120 mg,Cu (CuSO4·5H2O) 16 mg,Mn (MnSO4·H2O) 7 mg,Zn (ZnSO4·H2O) 80 mg,I (CaI2O6)0.7 mg,Se (Na2SeO3) 0.3 mg, TiO2 2.4 g。 2)总能、粗蛋白质、氨基酸、钙和总磷为实测值,消化能和标准全消化道可消化磷为参照NRC(2012)所得计算值。GE, CP, amino acids, Ca and TP were measured values, while DE and STTD P were calculated values according to NRC (2012). |
1.3 饲养管理
1.4 样品采集
1.5 指标测定及方法
1.5.1 生长性能和腹泻率
1.5.2 脏器指数
1.5.3 血清免疫球蛋白含量和肠道通透性指标
1.5.4 肠道形态
1.5.5 营养物质表观消化率
1.6 数据统计分析
2 结果
2.1 水溶性玉米蛋白粉替代豆粕对断奶仔猪生长性能和腹泻率的影响
表3 水溶性玉米蛋白粉替代豆粕对断奶仔猪生长性能和腹泻率的影响Table 3 Effects of water-soluble corn gluten meal replacing soybean meal on growth performance and diarrhea rate of weaned piglets |
| 项目 Items | 对照组 Control group | 试验Ⅰ组 Experimental group Ⅰ | 试验Ⅱ组 Experimental group Ⅱ | P值 P-value |
|---|---|---|---|---|
| 第1~14天Days 1 to 14 | ||||
| 初重Initial BW/kg | 7.84±0.03 | 7.85±0.03 | 7.84±0.03 | 0.945 |
| 末重Final BW/kg | 11.25±0.18 | 10.73±0.27 | 10.90±0.19 | 0.263 |
| 平均日增重ADG/g | 243.37±13.58 | 205.23±20.70 | 218.92±14.05 | 0.283 |
| 平均日采食量ADFI/g | 335.96±14.45 | 307.77±23.36 | 320.59±20.62 | 0.627 |
| 料重比F/G | 1.39±0.02 | 1.52±0.04 | 1.47±0.03 | 0.077 |
| 腹泻率Diarrhea rate/% | 8.97±1.47 | 7.75±1.85 | 8.21±1.90 | 0.906 |
| 第15~28天Days 15 to 28 | ||||
| 末重Final BW/kg | 17.72±0.61 | 17.25±0.77 | 17.30±0.62 | 0.870 |
| 平均日增重ADG/g | 462.05±34.51 | 466.68±34.99 | 457.52±32.71 | 0.984 |
| 平均日采食量ADFI/g | 679.27±40.37 | 636.18±45.14 | 632.40±37.70 | 0.695 |
| 料重比F/G | 1.48±0.04 | 1.37±0.02 | 1.39±0.04 | 0.168 |
| 腹泻率Diarrhea rate/% | 1.63±0.85 | 3.27±0.97 | 4.29±1.08 | 0.183 |
| 第1~28天Days 1 to 28 | ||||
| 平均日增重ADG/g | 352.95±21.76 | 335.84±25.98 | 338.22±22.33 | 0.866 |
| 平均日采食量ADFI/g | 507.59±25.56 | 471.97±33.26 | 476.49±26.28 | 0.656 |
| 料重比F/G | 1.43±0.02 | 1.41±0.01 | 1.41±0.03 | 0.692 |
| 腹泻率Diarrhea rate/% | 5.30±0.89 | 5.51±0.83 | 6.25±1.17 | 0.802 |
同行数据肩标不同字母表示差异显著(P<0.05),相同字母或无字母表示差异不显著(P>0.05)。下表同。 | |
In the same row, values with different letter superscripts mean significant difference (P<0.05), while with the same letter or no letter superscripts mean no significant difference (P>0.05). The same as below. |
2.2 水溶性玉米蛋白粉替代豆粕对断奶仔猪脏器指数的影响
表4 水溶性玉米蛋白粉替代豆粕对断奶仔猪脏器指数的影响Table 4 Effects of water-soluble corn gluten meal replacing soybean meal on organ indices of weaned piglets |
| 项目 Items | 对照组 Control group | 试验Ⅰ组 Experimental group Ⅰ | 试验Ⅱ组 Experimental group Ⅱ | P值 P-value |
|---|---|---|---|---|
| 心脏指数Heart index | 0.58±0.03 | 0.62±0.05 | 0.63±0.04 | 0.696 |
| 肝脏指数Liver index | 3.24±0.15 | 3.28±0.10 | 3.23±0.16 | 0.961 |
| 脾脏指数Spleen index | 0.30±0.04 | 0.31±0.03 | 0.23±0.01 | 0.133 |
| 肺脏指数Lung index | 1.42±0.08 | 1.66±0.08 | 1.50±0.10 | 0.177 |
| 肾脏指数Kidney index | 0.63±0.04 | 0.67±0.03 | 0.62±0.02 | 0.448 |
2.3 水溶性玉米蛋白粉替代豆粕对断奶仔猪血清免疫球蛋白含量和肠道通透性指标的影响
表5 水溶性玉米蛋白粉替代豆粕对断奶仔猪血清免疫球蛋白含量和肠道通透性指标的影响Table 5 Effects of water-soluble corn gluten meal replacing soybean meal on serum immunoglobulin contents and intestinal permeability indices of weaned piglets |
| 项目 Items | 对照组 Control group | 试验Ⅰ组 Experimental group Ⅰ | 试验Ⅱ组 Experimental group Ⅱ | P值 P-value |
|---|---|---|---|---|
| 免疫球蛋白A IgA/(μg/mL) | 26.35±0.85 | 27.69±0.71 | 26.45±0.44 | 0.325 |
| 免疫球蛋白G IgG/(μg/mL) | 306.71±9.20b | 400.13±3.39a | 415.11±5.95a | 0.001 |
| 免疫球蛋白M IgM/(μg/mL) | 29.85±1.81 | 34.17±1.12 | 33.51±1.63 | 0.129 |
| D-乳酸D-LA/(μg/mL) | 694.50±21.88 | 672.75±17.62 | 695.52±17.96 | 0.636 |
| 二胺氧化酶DAO/(pg/mL) | 254.69±9.71b | 317.56±9.21a | 319.78±13.54a | 0.001 |
2.4 水溶性玉米蛋白粉替代豆粕对断奶仔猪肠道形态的影响
表6 水溶性玉米蛋白粉替代豆粕对断奶仔猪肠道形态的影响Table 6 Effects of water-soluble corn gluten meal replacing soybean meal on intestinal morphology of weaned piglets |
| 项目 Item | 对照组 Control group | 试验Ⅰ组 Experimental group Ⅰ | 试验Ⅱ组 Experimental group Ⅱ | P值 P-value |
|---|---|---|---|---|
| 十二指肠Duodenum | ||||
| 绒毛高度Villus height/μm | 487.88±27.92 | 496.11±42.59 | 499.03±28.68 | 0.971 |
| 隐窝深度Crypt depth/μm | 273.22±13.73 | 267.95±12.65 | 246.20±9.84 | 0.281 |
| 绒隐比V/C | 1.82±0.15 | 1.90±0.24 | 2.05±0.17 | 0.679 |
| 空肠Jejunum | ||||
| 绒毛高度Villus height/μm | 429.00±22.73 | 526.35±41.54 | 463.18±19.31 | 0.093 |
| 隐窝深度Crypt depth/μm | 194.99±12.41b | 216.34±9.94ab | 245.65±8.51a | 0.012 |
| 绒隐比V/C | 2.26±0.22 | 2.48±0.26 | 1.90±0.11 | 0.175 |
| 回肠Ileum | ||||
| 绒毛高度Villus height/μm | 327.78±16.11 | 384.09±17.34 | 362.12±26.54 | 0.182 |
| 隐窝深度Crypt depth/μm | 188.30±6.33 | 201.55±7.00 | 206.49±5.86 | 0.151 |
| 绒隐比V/C | 1.76±0.12 | 1.91±0.06 | 1.76±0.14 | 0.584 |
2.5 水溶性玉米蛋白粉替代豆粕对断奶仔猪营养物质表观消化率的影响
表7 水溶性玉米蛋白粉替代豆粕对断奶仔猪营养物质表观消化率的影响Table 7 Effects of water-soluble corn gluten meal replacing soybean meal on nutrient apparent digestibility of weaned piglets |
| 项目 Items | 对照组 Control group | 试验Ⅰ组 Experimental group Ⅰ | 试验Ⅱ组 Experimental group Ⅱ | P值 P-value |
|---|---|---|---|---|
| 总能GE | 79.20±0.97 | 80.30±1.00 | 79.16±0.55 | 0.582 |
| 粗蛋白质CP | 65.28±1.54 | 67.74±1.86 | 65.46±1.43 | 0.502 |
| 粗脂肪EE | 86.29±1.55 | 81.72±1.57 | 85.46±1.93 | 0.156 |
| 有机物OM | 80.34±0.95 | 81.60±1.02 | 81.00±0.48 | 0.583 |
| 粗灰分Ash | 30.61±2.93 | 30.79±3.16 | 30.13±1.77 | 0.984 |
