This experiment was conducted to study the effects of different corn feed forms on intestinal microflora of geese. One hundred and ninety-two 7-day-old healthy Yangzhou geese were randomly assigned to 4 groups with 4 replicates and 12 geese in each replicate. In the case of the same composition of the diets, the groups were received mash corn diet, mash corn diet-whole corn diet, whole corn diet-mash corn diet, whole corn diet, respectively. The genomic DNA was extracted from the content of various intestinal segments at the age of 84 days. The V3 variable region of bacterial 16S rDNA was amplified with universal primer. Denaturing gradient gel electrophoresis profiles of the amplicons were analyzed. Results showed that whole corn diet decreased the bacterial species in jejunum but increased the microbial species in ileum. However, it had no significant effects on microflora in duodenum and cecum of geese. The results indicated that corn diet forms affected intestinal microflora of jejunum and ileum mostly, rectum followed and then duodenum and cecum of geese.[Chinese Journal of Animal Nutrition, 2010,22(1):169-175]
HU Ping1,SHI Shourong2,WANG Zhiyue1*,LIU Beiyi1,YANG Haiming1,KONG Xiangling1
. Effects of Different Corn Diet Forms on Intestinal Microflora of Geese by Denaturing Gradient Gel Electrophoresis[J]. Chinese Journal of Animal Nutrition, 2010
, 22(01)
: 169
-175
.
DOI: 10.3969/j.issn.1006-267x.2010.01.026