This experiment was to study the effects of intraduodenal infusion of soybean small peptides (SSP) and free amino acids (FAA) on absorption of peptides in small intestine of dairy goats. In a crossover design, 4 dairy goats with body weight of (38.38±3.09) kg fitted with permanent cannulas at the proximal duodenum and implanted with indwelling catheters in carotid artery, proximal and distal mesenteric vein and portal vein were used. The treatments were intraduodenally infused with 60 g/d SSP (SSP group) and AA in which contained the same quantity of individual FAA with 60 g SSP (AA group). The results showed that the mesenteric-drained viscera (MDV) net fluxes of total peptide-binding amino acid (PAA) in SSP group was significantly higher than that in AA group (P<0.05), the portal-drained viscera (PDV) net fluxes of total PAA in the SSP group was higher than that in AA group, but there was no significant difference between the SSP group and the AA group (P<0.05). The MDV and PDV net fluxes of total FAA in AA group were significantly higher than those in SSP group (P<0.05). These results showed that the increase of quantity of peptide in the small intestine promoted the absorption of peptide in the small intestine of dairy goats.［Chinese Journal of Animal Nutrition, 2010，22（2）:318-326］