The study was conducted to examine the feasibility of spectrofluorimetry used to study the phagocytosis of rumen protozoa on bacteria. Four healthy Xuhuai goats with permanent ruminal cannulas were selected as experimental animals to obtain rumen protozoa and bacteria. The fluorescence intensity of bacteria labeled by DAPA was tested to study the phagocytosis of rumen protozoa on bacteria. The results showed that there was linear correlation between the concentration of bacteria concentration labeled by DAPI and their fluorescence intensity (R2=0.995 2). The grazing rate of protozoa was 370.89 cells/(cell·h) for Xuhuai goats fed the diet with forage∶concentration ratio of 7∶3. There was volatility on grazing rate of rumen protozoa on bacteria of Xuhuai goats. Grazing quantity reached saturation on 60 min and the largest grazing quantity was 370.89 cells/cell. It was concluded that spectrofluorimetry could be used in studying grazing rate of rumen protozoa on bacteria.［Chinese Journal of Animal Nutrition, 2010，22（3）:611-615］