The experiment studied the regularity of digestibility and metabolism in male mink fed different protein level diets during fur development period. Sixty healthy male mink of 90 d old were randomly divided into 6 groups with 10 replicates and 1 mink per replicate. The mink in 6 groups were fed with protein levels of 26% (group Ⅰ), 28% (group Ⅱ), 30% (group Ⅲ), 32% (group Ⅳ), 34% (group Ⅴ) and 36% (group Ⅵ), respectively. The experiment lasted for 56 days, including preset period for 7 days and test period for 49 days. The results showed as follows: mink in different groups had no significant difference in feed intake, dry matter intake, dry matter output, dry matter digestibility, protein digestibility, fat digestibility, urine nitrogen content, net protein utilization and biological value of protein (P>0.05), but the protein digestibility was the highest when protein levels arrived at 32%; the digestibility of calcium in groups Ⅲ and Ⅵ was significantly higher than that in groups Ⅰ, Ⅱ, Ⅳ and Ⅴ (P<0.05); the digestibility of phosphorous in groups Ⅲ～Ⅵ was significantly higher than that in groups Ⅰ and Ⅱ (P<0.05); the nitrogen intake in groups Ⅲ～Ⅵ was extremely significantly higher than that in groups Ⅰ and Ⅱ (P<0.01); the fecal nitrogen contents in groups Ⅰ and Ⅱ were significantly lower than those in groups Ⅲ～Ⅵ (P<0.05); the nitrogen retention in groups Ⅰ and Ⅱ was significantly lower than that in group Ⅵ (P<0.05), and the nitrogen retention in group Ⅱ was significantly lower than that in group Ⅳ (P<0.05). In conclusion, when the protein level of diet arrived at 32%, the mink had the best protein digestibility and availability. So, the 32% protein level of diet was met to the protein need of mink during fur development period.［Chinese Journal of Animal Nutrition, 2010，22（3）:723-728］