In this study, we constructed an uncut intestine cDNA library, and examined nutrition-related gene expression in this library as a preliminary analysis. We used the whole intestinal tract tissue of M. albus and Invitrogen’s Gateway technology to construct the cDNA library and to perform scale expressed sequence tag (EST) sequencing and analysis. Our results showed that the library capacity reached 1.46×107 CFU, and the percentage of recombination was as high as 96.88%. PCR results showed that the average insert size was 2.11 kb. A total of 1 056 randomly selected cDNA clones were sequenced, and 906 were used in high-quality EST. Of the 906 cDNA clones, 886 were longer than 400 bp, with a splicing assembly of 762 unigenes, including 61 contigs and 701 singletons. We identified 5 categories within 59 species of putative nutrition-related genes based on their functional classification. These categories included 25 species for amino acid metabolism, 13 species for carbohydrate metabolism, 5 species for lipid metabolism, 12 species for energy metabolism, and 4 species for inorganic ion metabolism. In this study, we successfully constructed an uncut intestine cDNA library, and performed EST sequencing and analysis, which identified nutrition-related EST. This study provided the basic data for follow-up researches related to nutrition in M. albus.［Chinese Journal of Animal Nutrition, 2010，22（4）:992-999］