This study was conducted to study the effects of nutrient digestibility and growth performance of growing minks. Sixty healthy male mink at the age of 45-day-old were randomly divided into 6 groups with 10 replicates in each group and 1 mink per replicate. The mink in 6 groups were fed experimental diets with protein levels of 28% (group Ⅰ), 30% (group Ⅱ), 32% (group Ⅲ), 34% (group Ⅳ), 36% (group Ⅴ) and 38% (group Ⅵ), respectively. The trial lasted for 60 days. The results showed as follows: the feed intake and dry matter (DM) intake of the groups Ⅵ, Ⅴ and Ⅵ were significantly higher than those of group Ⅱ (P<0.05). The digestibility of DM, protein and fat of group Ⅳ were significantly higher than those of groups Ⅰ and Ⅱ (P<0.05 or P<0.01). No significant difference on digestibility of DM, protein and fat were found between group Ⅳ and other groups (P>0.05). The P digestibility of group Ⅳ was significantly higher than that of groups Ⅰ and Ⅴ (P<0.05), but there was no significant difference between group Ⅳ and the others (P>0.05). With the increase of dietary protein levels, nitrogen intake and urine nitrogen were increased (P<0.05 or P<0.01). The fecal nitrogen, nitrogen deposition, net protein utilization, biological value of protein were not significantly affected by dietary protein levels (P>0.05). The average daily gain and F/G of groups Ⅳ, Ⅴ and Ⅵ were significantly greater than those of group Ⅰ (P<0.01). In conclusion, when the protein level of diet arrived at 34%, the nutrient digestibility, average daily gain and F/G of growing minks were optimal.［Chinese Journal of Animal Nutrition, 2010，22（4）:1101-1106］