To improve the quality of silage and reduce the cost of microbial inoculants, molecular biological assay was carried out on a major strain of lactic acid bacteria called GLP01, which was isolated from a silage cellar in Germany, and the culture conditions were optimized. One-factor-at-a-time approach was used to investigate the effects of different medium components (carbon source and nitrogen source) and different culture conditions (temperature, initial pH, and inoculum size) on growth of GLP01; response surface technique (RST) was applied to the optimization of culture condition to get the growth model and factor levels of the optimal model value. The results showed as follows: the optimal carbon source and nitrogen source were fructose and yeast powder, respectively; the optimal culture conditions of GLP01 were pH 5.47, temperature 35.3 ℃, and inoculum size 8.16%. In conclusion, lactic acid bacteria GLP01 can be used as silage microbial inoculants, but the effect remains to be clarified.［Chinese Journal of Animal Nutrition, 2010，22（5）:1307-1313］