The experiment was conducted to study the effects of the milk component precursors (MCP) concentration in external pudendal artery on nutrients uptake in mammary gland and the expression of related hormone receptor mRNA in mammary tissues of dairy goat. Three dairy goats aged from 2 to 3 years with similar body weight and milk yield, and intubated in the external pudendal artery and the abdominal subcutaneous vein, were selected in this study. A mixture of amino acids and glucose was infused into the external pudendal artery. Arteriovenous plasma and mammary tissues were collected to measure the contents of total protein (TP), albumin (ALB) and the mRNA expression levels of β-casein, prolactin receptor (PRLR), growth hormone receptor (GHR) and insulin-like growth factor Ⅰ (IGF-Ⅰ) in mammary tissues. The result was showed as follows: the concentration of MCP in arteriovenous plasma and the ingestion by to the mammary gland were increased after infusion (P<0.05). The mRNA expression levels of β-casein (P<0.05), GHR (P<0.05), IGF-Ⅰ (P<0.05) and PRLR (P<0.01) in mammary tissues were significantly increased. The infusion increase of amino acids and glucose can increase the concentration of MCP, efficiently stimulate the changes of hormone in the blood, and elevate the expression of the related hormone receptor mRNA in mammary tissues.［Chinese Journal of Animal Nutrition, 2010，22（5）:1314-1319］