This experiment was conducted to explore the feasibility of substituting for antibiotics with probiotics, origanum oil and through elevating dietary vitamin E, vitamin B family and micro elements levels. Three hundred and twenty-four crossbred (Landrace×Yorkshine) barrows with average body weight of 27 kg were randomly assigned to 6 dietary groups: group Ⅰ (chlorotetracycline group, added 50 mg/kg chlortetracycline in basal diet); group Ⅱ (origanum oil group, added 20 mg/kg oreganum oil in basal diet); group Ⅲ (probiotics group, added 1.5 g/kg probiotics in basal diet); group Ⅳ (nutrient enhancement group, elevated dietary vitamin E, vitamin B family and micro elements Zn, Mn, I); group Ⅴ (origanum oil+nutrient enhancement group); group Ⅵ (probiotics+nutrient enhancement group). The trial lasted for 90 days. The results showed that pigs in group Ⅴ recorded the highest ADG, which was significantly higher than that in group Ⅳ and group Ⅵ (P<0.05), and had the best F/G. The number of white blood cells in group Ⅲ was significantly higher than that in group Ⅳ and group Ⅴ (P<0.05). The lymphocyte transformation rate in group Ⅲ and group Ⅴ was significantly higher than that in group Ⅰ, group Ⅱ and group Ⅳ (P<0.05). The activity of GSH-Px in group Ⅰ was the highest, while the activity of CAT in group Ⅴ was the highest. Antibody levels of classical swine fever, porcine reproductive and respiratory syndrome and foot-and-mouth disease had no difference among groups (P>0.05). These results indicated that origanum oil+nutrient enhancement could successfully substitute for antibiotics, and pigs fed diets added origanum oil and elevated dietary vitamin E, vitamin B family and micro elements could obtain better performance, have better disease-resistant and stress-resistant ability than pigs fed diets supplemented with chlortetracycline.［Chinese Journal of Animal Nutrition, ２００9，２1（5）:645-651］