The objective of this study was to investigate the effects of avian pancreatic polypeptide (APP) on expression of avian pancreatic polypeptide receptor (APPR) mRNA in livers and small intestines of broilers. Crude extract liquid of APP and chromatographic APP was used as test material, and ninety 1-week-old broilers were randomly allocated to 5 groups with 3 replicates of 6 broilers. Group Ⅰ was control group; group Ⅱ and group Ⅲ were fed APP; group Ⅳ and  group Ⅴ were injected hypodermically APP. After 7 weeks, the broilers were slaughtered randomly in each treatment to determine the relative expression of APPR mRNA in the livers and small intestines of broilers by SYBR GreenⅠRT-PCR. The results showed that, compared with control group, the expression of APPR mRNA decreased in the livers and small intestines of the other groups, the decreased degree in injection groups was more significant than that in feed groups; and also the impact of chromatographic APP was more significant than that of crude extract liquid of APP, which suggesting that supplementing APP could inhibitie the expression of APPR mRNA. In conclusion, the above results could provide some theoretical basis for the further research on the relationship between APP and APPR to elucidate physiological function and mechanism.