This experiment was conducted to study the effects of glutamine (Gln) and chitosan (CS) on small intestine disaccharidase activity, intestine villus height and faecal microflora of weanling piglets. One hundred and ninetytwo 21-day-old weanling piglets with an average body weight of (5.27±0.13) kg were randomly allocated into 4 treatments with 3 replicates in each treatment and 16 piglets in each replicate. The experiment which lasted for 14 days included two phases: 21～28 d and 29～35 d. The treatments were basal diet (CT), basal diet＋1.00% glutamine (GlnT), basal diet＋0.4% chitosan (CST) and basal diet＋1.00% glutamine＋0.04% chitosan (GCT), respectively. Results were as followed: 1) compared with CT, duodenum lactase and maltase activities, jejunum lactase and sucrase activities, ileum lactase and sucrase activities were significantly increased in GCT (P<0.05); 2) the intestine villus height from CST, GlnT to GCT was increased, but the differences between treatments were not significant （P>0.05）; 3) the quantities of E.coli and Staphylococci in GCT decreased but those of Lactobacillus increased whereas the differences were not significant （P>0.05）. These results indicated that the addition of 1.00% glutamine and 0.04% chitosan significantly increased small intestine disaccharidase activities (P<0.05) and intestine villus height and improved faecal microflora, and the effects were better than single addition.