This experiment was conducted to study the requirements of vitamins and minerals for Apis cerana cerana during overwintering period. We detected the contents of vitamins and minerals in honey, which was the natural fodder for Apis cerana cerana during overwintering period, and designed the experimental diets, including medium dose vitamin group, high dose vitamin group, medium dose mineral group, high dose mineral group, high dose vitamin and medium dose mineral group, and medium dose vitamin and high dose mineral group. The effects of these experimental groups were compared with the control group of honey and sugar water (syrup). Feed consumption and reduction rate of population of swarm were detected. The results showed that the dose of complex vitamins in overwintering diet of Apis cerana cerana in syrup should be lower than 0.08 mg/kg and that of complex minerals should be between 0.95 and 1.89 mg/kg when there was no natural fodder-honey.［Chinese Journal of Animal Nutrition, 2010，22（6）:1625-1629］