The study was conducted to investigate the effect of dietary ideal protein level on tenderness and mRNA expression levels of P94-NFκB signaling pathway related to proteins and CAST of longissimus dorsi in finishing pigs. Ninety crossbred pigs (Duroc × Landrace × Large white, about 50 kg BW) were randomly allocated into 3 treatments with 3 replicates per treatment and 10 heads per replicate. Pigs in the three treatments were fed diets with the same energy level and ideal protein levels at 12%, 16% and 20%, respectively. All pigs were slaughtered after 58 days experiment and then muscle samples were collected for the analysis of shear force, meanwhile, mRNA expression levels of P94, NFκB, IκB and CAST of longissimus dorsi in pigs were determined by real-time PCR. The results showed as follows: 1) there were significant effects of dietary protein level on shear force and the mRNA expression levels of P94, NFκB, IκB and CAST of longissimus dorsi (P<0.05); with the increasing of dietary protein level, the shear force and mRNA expression level of IκB and CAST were increased, but that of P94 and NFκB were decreased. 2) the shear force was positively correlated with the mRNA expression levels of IκB (coefficient correlation=0.513, P<0.05) and CAST (coefficient correlation=0.816, P<0.01). 3) The mRNA expression level of CAST was negatively correlated with that of P94 (coefficient correlation=－0.496, P<0.05), but positively correlated with that of IκB (coefficient correlation=0.710, P<0.01). 4) The mRNA expression level of P94 was positively correlated with that of NFκB (coefficient correlation=0.550, P<0.05), but negatively correlated with that of IκB (coefficient correlation=－0.518, P<0.05); the mRNA expression levels of IκB and NFκB were negatively correlated (coefficient correlation=－0.539, P<0.05) to each other. The results indicate that high dietary protein level can increase the shear force and the mRNA expression levels of CAST and IκB, but decrease the tenderness and the mRNA expression level of P94; P94-NFκB signaling pathway plays a role in the process of regulating muscle tenderness, but it is not the main pathway.［Chinese Journal of Animal Nutrition, 2011, 23（8）：1342 -1350］
ZHANG Yong,CUI Yan,TAO Liang,ZHU Yujing,DENG Ke,SUN Cui,SHAO Caimei
. Dietary Protein Level Affects the Tenderness and P94-NFκB Signaling Pathway of Longissimus Dorsi in Finishing Pigs[J]. Chinese Journal of Animal Nutrition, 2011
, 23(08)
: 1342
-1350
.
DOI: 10.3969/j.issn.1006-267x.2011.08.013
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