The objective of this study was to compare the effects of different selenium (Se) sources and levels on growth performance, tissue Se content and antioxidant capacity of broilers, and to investigate the feasibility of using 0.20 mg/kg organic Se replacing 0.30 mg/kg inorganic Se in poultry production. Six hundred 1-day-old Arbor Acres broilers were randomly assigned into five groups with six replicates per group and twenty broilers per replicate. The broilers were fed a basal diet without Se supplementation (control) or the basal diet adding 0.30 mg/kg Se in the form of sodium selenite (SS), 0.20 mg/kg Se in the form of Se-yeast (SYⅠ), 0.30 mg/kg Se in the form of Se-yeast (SY Ⅱ) and 0.30 mg/kg mixed Se (0.15 mg/kg SS+0.15 mg/kg SY) (MS), respectively. The trial lasted for 42 days. The results showed as follows: 1) average daily feed intake (ADFI) of broilers in SYⅡ group was significantly lower than that in the control group in the starter period (1 to 21 days) (P<0.05), and adding organic selenium significantly decreased ADFI of broilers in the whole period (1 to 42 days) (P<0.05 or P<0.01). 2) Compared with the SS group and SS group, adding organic selenium significantly increased Se content in breast muscle of broilers (P<0.05 or P<0.01), and Se content in SYⅡ group was significantly higher that in the other groups (P<0.01). Plasma Se content of broilers in SYⅠ and SYⅡ groups was significantly higher than that in the control group (P<0.05 or P<0.01). Adding organic selenium significantly increased Se content in liver of broilers (P<0.05 or P<0.01). 3) In the starter period, plasma glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) activities of broilers in SYⅡ group were significantly increased compared with SS group (P<0.05); the T-SOD activity in liver of broilers in SS, SYⅠ and SYⅡ groups was significantly increased compared with the control group (P<0.05 or P<0.01). In the finishing period (22 to 42 days), the GSH-Px activity in plasma and liver of broilers in SS, SYⅠ and SYⅡ groups was significantly increased compared with the control group (P<0.05 or P<0.01), plasma malondialdehyde content of broilers in the groups added organic Se was significantly decreased compared with the other groups (P<0.05 or P<0.01), and the activities of catalase (P<0.05) and GSH-Px (P<0.01) in liver in MS group were significantly increased compared with the control group. In conclusion, by increasing tissue Se content, the improvement of growth performance and antioxidant capacity of broilers in SYⅡ group is the best. Furthermore, 0.30 mg/kg inorganic Se replaced by 0.20 mg/kg organic Se has the same effects on improving growth performance, antioxidant capacity and tissue selenium content of broilers.
TIAN Jinke
,
HUSSAIN Ahmad
,
LI Wei
,
GAO Shang
,
WANG Tian
. Different Selenium Sources and Levels Affect Tissue Selenium Content and Antioxidant Capacity of Broilers[J]. Chinese Journal of Animal Nutrition, 2012
, 24(6)
: 1030
-1037
.
DOI: 10.3969/j.issn.1006-267x.2012.06.006
[1] KIM Y Y.Differences in biological activity and metabolism of selenium due to its chemical form[J].Animal Science and Technology (Korea),2000,42(6):835-848.
[2] SURAI P.Selenium in poultry nutrition 2.Reproduction,egg and meat quality and practical applications[J].World's Poultry Science Journal,2002,58:431-450.
[3] HOFFMAN D J.Role of selenium toxicity and oxidative stress in aquatic birds[J].Aquatic Toxicology,2002,57:11-26.
[4] SPALLHOLZ J E.On the nature of selenium toxicity and carcinostatic activity[J].Free Radical Biology and Medicine,1994,17:45-64.
[5] SPALLHOLZ J E,PALACE V P,REID T W.Methioninase and selenomethionine but not Se-methylselenocysteine generate methylselenol and superoxide in an in vitro chemiluminescent assay:implications for the nutritional carcinostatic activity of selenoamino acids[J].Biochemical Pharmacology,2004,67:547-554.
[6] WANG Y B,XU B H.Effect of different selenium source (sodium selenite and selenium yeast) on broiler chickens[J].Animal Feed Science and Technology,2008,144:306-314.
[7] MAHMOUD K Z,EDENS F W.Influence of organic selenium on hsp70 response of heat-stressed and enteropathogenic Escherichia coli-challenged broiler chickens (Gallus gallus)[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2005,141:69-75.
[8] 夏枚生,张红梅,胡彩虹,等.纳米硒对肉鸡生长和抗氧化的影响[J].营养学报,2005,27(4)307-310.
[9] WANG Y X,ZHAN X A,ZHANG X W,et al.Comparison of different forms of dietary selenium supplementation on growth performance,meat quality,selenium deposition,and antioxidant property in broilers[J].Biological Trace Element Research,2011,143(1):261-273.
[10] PAYNE R L,SOUTHERN L L.Changes in glutathione peroxidase and tissue selenium concentrations of broilers after consuming a diet adequate in selenium[J].Poultry Science,2005,84:1268-1276.
[11] SEV CKOV S,SK R IVAN M,DLOUH G,et al.The effect of selenium source on the performance and meat quality of broiler chickens[J].Czech Journal of Animal Science,2006,51:449-457.
[12] ZHAN X A,WANG M,ZHAO R Q,et al.Effects of different selenium source on selenium distribution,loin quality and antioxidant status in finishing pigs[J].Animal Feed Science and Technology,2007,132:202-211.
[13] JUNIPER D T,PHIPPS R H,RAMOS-MORALES E,et al.Effects of dietary supplementation with selenium enriched yeast or sodium selenite on selenium tissue distribution and meat quality in lambs[J].Animal Feed Science and Technology,2009,149:228-239.
[14] JUNIPER D T,PHIPPS R H,RAMOS-MORALES E,et al.Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on selenium tissue distribution and meat quality in beef cattle[J].Journal of Animal Science,2008,86:3100-3109.
[15] 丁文杰,戴晋军,杨志龙,等.富硒猪肉的研究进展[J].饲料与畜牧:新饲料,2008,7:55-57.
[16] PAN C L,HUANG K H,ZHAO Y X,et al.Effect of selenium source and level in hen's diet on tissue selenium deposition and egg selenium concentrations[J].Journal of Agricultural and Food Chemistry,2007,55:1027-1032.
[17] VIGNOLA G,LAMBERTINI L,MAZZONE G,et al.Effects of selenium source and level of supplementation on the performance and meat quality of lambs[J].Meat Science,2009,81:678-685.
[18] TEMPLE N J.Antioxidants and disease:more questions than answers[J].Nutrition Research,2000,20:449-459.
[19] FANG Y Z,YANG S,WU G Y.Free radicals,antioxidants,and nutrition[J].Nutrition,2002,18:872-879.
[20] 高建忠,黄克和.动物硒蛋白研究进展[J].畜牧与兽医,2004,36(7):39-42.
[21] MIKULSKI D,JANKOWSKI J,ZDUCZYK Z,et al.The effect of selenium source on performance,carcass traits,oxidative status of the organism,and meat quality of turkeys[J].Journal of Animal and Feed Science,2009,18:518-530.
