This study was conducted to investigate the changes of activities of antioxidant enzymes, contents of non-enzymic antioxidant substances, as well as the anti-superoxide anion free radical ability (ASA) and anti-hydroxyl free radical ability (AHA) in juvenile Jian carp (Cyprinus carpio var. Jian) with different body weights (18 to 75 g). A total of 750 Jian carp with an average initial body weight of (18.0?0.2) g were randomly distributed into 5 groups with 3 replicates per group and 50 fish per replicate. The fish in the 5 groups were fed the same diets and were sampled every 14 days (total of 5 times). Briefly, after weighing, the blood, hepatopancreas, intestine and muscle were quickly collected for antioxidant indices analysis. The results showed as follows: contents of malondialdehyde (MDA) and protein carbonyls (PCA) in serum, hepatopancreas, intestine and muscle were significantly decreased with increasing body weight up to 31.4 and 58.6 g (P<0.05), respectively, and no differences were found with further increase of body weight (P>0.05). Adversely, ASA and AHA in serum and studied tissues were increased with increasing body weight up to 40.1 g (P<0.05), and plateaued thereafter (P>0.05). Interestingly, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) took the same trends with ASA. Similarly, activities of glutathione S-transferase (GST) and glutathione reductase (GR) in serum and tissues were all increased with increasing body weight up to 31.4 g (P<0.05), and plateaued thereafter (P>0.05). Furthermore, reduced glutathione (GSH) content in serum, hepatopancreas, intestine and muscle were also increased with increasing body weight up to 31.4, 31.4, 31.4 and 58.6 g (P<0.05), respectively, and no changes were found with further increasing body weight (P>0.05). Summarily, the results in this study indicate that in the juvenile period of Jian carp (18 to 75 g), the activities of SOD, CAT, GSH-Px, GST and GR, as well as GSH content in serum and tissues are increased with increasing body weight, and thus the ASA and AHA are also improved, and finally products of the lipid peroxidation and protein oxidation are decreased with increasing body weight until reaching steady state.
LIU Yang
,
CHI Lei
,
FENG Lin
,
JIANG Jun
,
ZHOU Xiaoqiu
. Changes of Antioxidant Indices in Serum and Tissues of Jian Carp with Different Body Weights[J]. Chinese Journal of Animal Nutrition, 2012
, 24(8)
: 1493
-1502
.
DOI: 10.3969/j.issn.1006-267x.2012.08.013
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