This study was aimed to identify strain properties of Lactobacillus sp. GF103 and assess it as potential probiotics in vitro. Lactobacillus sp. GF103, which could inhibit the growth of pathogenic bacteria Escherichia coli and Staphylococcus aureus, was isolated from soil nearby Beijing Daxing breeding pig farm. And it was identified as Lactobacillus plantarum by 16S rRNA sequence analysis. Learned by the assessment of potential prebiotics in vitro, a large amount of the strain GF103 survived under the condition of pH=3. The strain GF103 had the ability to tolerance of 0.3% bile salt. The viable counts of strain GF103 did not change after 0.5 h (P>0.05) in simulated gastric fluid and decreased 1 log value after 3 h (P<0.01). The viable counts of strain GF103 did not decrease in simulated small intestinal fluid after 3 h (P>0.05). These results suggest that the strain GF103 has probiotic properties and the effects on its application in animals as feed additives can be further studied.
DONG Xiaoli
,
ZHANG Naifeng
,
ZHOU Meng
,
TU Yan
,
DIAO Qiyu
,
NIE Mingfei
. Isolation and Identification of Lactobacillus sp. GF103 and Assess It as Potential Probiotics in Vitro[J]. Chinese Journal of Animal Nutrition, 2012
, 24(9)
: 1832
-1838
.
DOI: 10.3969/j.issn.1006-267x.2012.09.030
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