This study was conducted to determine the effects of acetate concentration on triacylglycerol (TAG) content, as well as expression levels of leptin and peroxisome proliferator-activated receptor-γ (PPARγ) genes in bovine mammary epithelial cells (BMECs). The 3th passage BMECs with a density of 1×105 cells/well were cultured for 48 h, then cells were divided into six treatments. Culture mediums containing different concentrations of acetate (0, 4, 6, 8, 10 and 12mmol/L) were used in each treatment, respectively. Cells were continuous incubated in an incubator with 5% CO2 at 37 ℃ for 48 h, then were collected for the analysis of lipid droplet formation, TAG content, and expression levels of leptin and PPARγ genes. The results showed as follows: with the increase of acetate concentration, lipid droplets was increased in BMECs; TAG content and PPARγ gene expression level were significantly increased linearly or quadratically (P<0.05), and 10 and 12 mmol/L were optimal concentrations; leptin gene expression level was up-regulated by acetate at a certain concentration, and 8 and 10 mmol/L were optimal concentrations. In conclusion, acetate can stimulate lipid droplet formation, TAG accumulation and expression levels of leptin and PPARγ genes in BMECs; in the present study, 10 to 12 mmol/L acetate has better effects on PPARγ gene expression, and 8 to 10 mmol/L acetate has better effects on leptin gene expression.
QI Lizhi
,
SHENG Ran
,
YAN Sumei
,
ZHAO Yanli
. Effects of Acetate Concentration on Triacylglycerol Content and Expression Levels of Leptin and PPARγ Genes in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2013
, 25(7)
: 1519
-1525
.
DOI: 10.3969/j.issn.1006-267x.2013.07.016
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