Effects of Thiamine on Concentrations of Volatile Fatty Acids and Lactate in Culture Medium of High Concentrate Substrate after in Vitro Rumen Fermentation

ZHANG Jun; WANG Mengzhi; HAO Zhimin; YU Lihuai; WANG Hongrong

doi:10.3969/j.issn.1006-267x.2014.02.026

2014 , Vol. 26 >Issue 2: 489 - 495

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.026

Feed Science and Technology

Effects of Thiamine on Concentrations of Volatile Fatty Acids and Lactate in Culture Medium of High Concentrate Substrate after in Vitro Rumen Fermentation

ZHANG Jun ,
WANG Mengzhi ,
HAO Zhimin ,
YU Lihuai ,
WANG Hongrong

Expand

1. Yangzhou Yangda Kangyuan Dairy Co., Ltd., Yangzhou 225009, China;
2. Yangzhou University Experimental Farm, Yangzhou 225009, China;
3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2013-08-19

Online published: 2014-01-27

Fold

Abstract

This experiment was conducted to study the effects of thiamine on concentrations of volatile fatty acids and lactate in culture medium of high concentrate substrate after in vitro rumen fermentation. Four healthy Holstein dairy cows equipped with permanent ruminal cannulas were used to provide rumen fluid. The fermentation substrate was consist of casein, wheat starches, soluble starches, pectins, xylan, milled filter paper and carboxymethyl cellulose, and the concentrate to forage ratio was 6：4. The supplemental levels of thiamine were 0, 60, 120, 180 and 240 mg/kg, respectively. The results showed as follows: generally, the lactate concentration in culture medium was decreased by thiamine under the condition of high concentrate substrate, and 180 mg/kg group showed the best effect. It was also observed that 180 mg/kg group increased total volatile fatty acid concentration but decreased the ratio of acetate to propionate. It is concluded that the supplemental level of 180 mg/kg thiamine can stabilize rumen microbial fermentation of high concentrate substrate with 6：4 of the concentrate to forage ratio.

Key words： thiamine; rumen fermentation; lactate; volatile fatty acid

Cite this article

ZHANG Jun , WANG Mengzhi , HAO Zhimin , YU Lihuai , WANG Hongrong . Effects of Thiamine on Concentrations of Volatile Fatty Acids and Lactate in Culture Medium of High Concentrate Substrate after in Vitro Rumen Fermentation[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 489 -495 . DOI: 10.3969/j.issn.1006-267x.2014.02.026

References

[1] MUTSVANGWA T, WRIGHT, T C.Sub-acute ruminal acidosis(SARA)in dairy cows[M].Ontario:Ministry of Agriculture and Food, 2003.

[2] COMBS G F, Jr.The vitamins:fundamental aspects in nutrition and health[J].American Journal of Clinical Nutrition, 1991, 53(3):755-763.

[3] SHAVER R D, BAL M A.Effect of dietary thiamin supplementation on milk production by dairy cows[J].Journal of Dairy Science, 2000, 83(10):2335-2340.

[4] 张红伟.硫胺素(VB₁)对瘤胃内营养物质消化代谢的影响[D].硕士学位论文.扬州:扬州大学, 2010.

[5] DE OLIVEIRA L A, JEAN-BLAIN C, KOMISARCZUK-BONY S, et al.Microbial thiamin metabolism in the rumen simulating fermenter:the effect of acidogenic conditions, a high sulfur level and added thiamin[J].British Journal of Nutrition, 1997, 78(4):599-613.

[6] 熊本海, 卢德勋, 高俊.绵羊瘤胃VFA吸收效率及模型参数的研究[J].动物营养学报, 1999, 11(S1):248-255.

[7] 张龙翔, 张庭芳, 李令媛.生化实验方法和技术[M].2版.北京:高等教育出版社, 1997:422-428.

[8] BECHDEL S I, HONEYWELL H E, DURCHER R A, et al.Synthesis of vitamin B in the rumen of the cow[J].Journal of Biological Chemistry, 1928, 80(1):231-238.

[9] MILLER B L, MEISKE J C, GOODRICH R D.Effect of grain source and concentrate level on B-vitamin production and absorption in steers[J].Journal of Animal Science, 1986, 62(2):473-483.

[10] ZINN R A, OWEN F N, STUART R L, et al.B-vitamin supplementation of diets for feedlot calves[J].Journal of Animal Science, 1987, 65(1):267-277.

[11] TAJIMA K, ARAI S, OGATA K, et al.Rumen bacterial community transition during adaptation to high-grain diet[J].Anaerobe, 2000, 6(5):273-284.

[12] 韩昊奇, 刘大程, 高民, 等.不同NFC/NDF比对奶山羊瘤胃微生物及瘤胃pH值变化的影响[J].动物营养学报, 2011, 25(4):597-603.

[13] PREVOT S, SENAUD J, BOHATIER J, et al.Variation in the composition of the ruminal bacterial microflora during the adaptation phase in an artificial fermentor (Rusitec)[J].Zoological Science, 1994, 11:871-878.

[14] 冯仰廉.反刍动物营养学[M].北京:科学出版社, 2004.

[15] VAN HOUTERT M.Challenging the retinal for altering VFA ratios in growing ruminates[J].Feed Mix, 1996, 4(1):8-12.

[16] BANNINK A, FRANCE J, LOPEZ S, et al.Modelling the implications of feeding strategy on rumen fermentation and functioning of the rumen wall[J].Animal Feed Science and Technology, 2008, 143(1/2/3/4):3-26.

[17] ØRSKOV E R, GRUBB D A, SMITH J S, et al.Efficiency of utilization of volatile fatty acids for maintenance and energy retention by sheep[J].British Journal of Nutrition, 1979, 41(3):541-552.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References