Experimental Methods

Effects of Temperature and Time for Storage of Digestive Enzyme Preparation on Digestive Ability of Simulated Intestinal Fluid for Ducks in Bionic Digestion

  • ZHANG Li ,
  • ZHAO Feng ,
  • YAN Feng ,
  • WANG Yuming ,
  • LIU Chengling ,
  • ZHANG Hongfu
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  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agriculture Science, Beijing 100193, China

Received date: 2013-12-05

  Online published: 2014-05-30

Abstract

This experiment was conducted to study the effects of temperature and time for storage of digestive enzyme preparation on digestive ability of simulated intestinal fluid (SIF) for ducks in bionic digestion, which would provide a reference for the standardization of simulated digestive fluid. Storage temperature at 4 ℃ or room temperature and storage time at 0, 1, 2, 3, 4, 6, 8, 10 or 12 months were used in a 2×9 two-factorial completely randomized design. There were 18 treatments in this experiment. The activities of trypsin, chymotrypsin and α-amylase were determined in duck SIF from each treatment. The dry matter digestibility and enzymatic hydrolysate gross energy of corn, soybean meal and wheat bran were determined using bionic digestion system. The results showed as follows:1) the activity of α-amylase in duck SIF was significantly higher for the digestive enzyme preparation stored at 4 ℃ than stored at room temperature (P0.05). The activities of these 3 digestive enzymes in duck SIF significantly decreased as the storage time extended from 0 to 12 months for digestive enzyme preparation (P0.05). 3)The storage time of digestive enzyme preparation significantly affected the apparent digestibility and true digestibility of dry matter, and the apparent gross energy and true gross energy of enzymatic hydrolysate of corn, soybean meal and wheat bran (P<0.05). However, for the storage temperature of 4 ℃ or room temperature and the storage time extended from 0 to 10 months, the true digestibility of dry matter and the true gross energy of enzymatic hydrolysate of corn, soybean meal and wheat bran showed relatively stable (less than 1% and 0.10 MJ/kg of relative difference, respectively). In conclusion, although the storage time of digestive enzyme preparation is the main factor affecting the activities of digestive enzymes in duck SIF, however, the digestive abilities of SIF are stable for digestive enzyme preparation stored at 4 ℃ or room temperature and no more than 10 months.

Cite this article

ZHANG Li , ZHAO Feng , YAN Feng , WANG Yuming , LIU Chengling , ZHANG Hongfu . Effects of Temperature and Time for Storage of Digestive Enzyme Preparation on Digestive Ability of Simulated Intestinal Fluid for Ducks in Bionic Digestion[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1545 -1555 . DOI: 10.3969/j.issn.1006-267x.2014.06.015

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