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Effects of Additional L-Tryptophan, Fructan and Casein on Skatole Concentration in Pig Manure Fermentation Broths

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  • Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China

Received date: 2014-04-03

  Online published: 2014-09-10

Abstract

In order to reduce the pollution of pig skatole in vitro, this study investigated the effects of different doses of additional L-tryptophan (L-Trp), fructan and casein on skatole and its related compounds concentration in pig manure fermentation broths. Eighty Duroc×Landrace×Yorkshire pigs with (50.0±0.5) kg body weight were randomly allotted into control group and test group with 4 replicates per group and 10 pigs per replicate. Pigs were accordingly fed a basal diet and an adding 0.2% Bacillus subtilis test diet. After 6 weeks, 8 pigs in each group were randomly selected; their fresh manures were collected and prepared manure anaerobic broths for fermentation experiment. A 3×3 orthogonal design was used in the fermentation experiment. The additional doses of L-Trp, casein, fructan in fermentation broths were 0, 0.05% and 0.10%; 0, 0.125% and 0.250%; 0, 0.75% and 1.50%, respectively. The concentrations of Trp, indole-3-acetic acid (IAA), indole and skatole were detected by high performance liquid chromatography (HPLC) and microbial communities in fermented broths in blank control and blank test groups were detected by fluorescnce-based PCR-SSCP-FLP (F-PCR-SSCP-FLP) and capillary electrophoresis. The results showed as follows: 1) the concentrations of Trp, indole, IAA and skatole in test group were significantly decreased compared with the control group (P<0.05). 2) L-Trp was the main influence factor on Trp and skatole concentration in fermentation broths, respectively, casein was the main influence factor on indole concentration in the control group, and fructan was the main factor on IAA concentration in fermentation broths. 3) Additional L-Trp or fructan and Bacillus subtilis did not interact (P>0.05). 4) The bacteria Bacillus (CWBIB1434) was predominant and its content was 26% in the blank control group, and the Lactose Streptococcus (AF201899) was predominant and its content was 36% in the blank test group. The conclusion is that the reduction of Trp in pig manure and additional Bacillus subtilis in diet are beneficial to the abatement of skatole pollution in vitro.

Cite this article

SHENG Qingkai, CHENG Jianguo, ZHAO Hongbo, DOU Hongyan, XUAN Yujuan, WU Ying . Effects of Additional L-Tryptophan, Fructan and Casein on Skatole Concentration in Pig Manure Fermentation Broths[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2797 -2804 . DOI: 10.3969/j.issn.1006-267x.2014.09.043

References

[1] WESOLY R,WEILER U.Influences of feed and feed additives on the skatole formation and accretion in adipose tissue of pigs[J].Züchtungskunde,2012,84(5):412-426.  

[2] ZAMARATSKAIA G,SQUIRES E J.Biochemical,nutritional and genetic effects on boar taint in entire male pigs[J].Animal,2009,3(11):1508-1521.  

[3] 杨锋.枯草芽孢杆菌的抗逆特性及其对仔猪生化指标和氨气排放的影响[D].硕士学位论文.杭州:浙江工商大学,2011.

[4] 李彩燕.饲粮纤维对猪体粪臭素沉积的调控及分子机理研究[D].博士学位论文.杭州:浙江大学,2009.

[5] 夏枚生,胡彩虹,许梓荣.果寡糖对猪粪便细菌群作用下L-色氨酸代谢的影响[J].中国畜牧杂志,2004,40(1):11-13.

[6] 刘毅,徐维家,李妍.人工神经原网络处理PCR数据在细菌鉴定中的应用[J].中国微生态学杂志,2009,21(5):468-473.

[7] WEEMS J M,CUTLER N S,MOORE C,et al.3-methylindole is mutagenic and a possible pulmonary carcinogen[J].Toxicological Sciences,2009,112(1):59-67.  

[8] TERENCE R W,NEIL P P,HAROID L D,et al.Catabolic pathway for the production of skatole and indoleacetic acid by the acetogen Clostridium drakei,Clostridium scatologenes,and swine manure[J].Applied and Environmental Microbiology,2008,74(6):1950-1953.  

[9] LI C Y,LIU J X,WANG Y Z,et al.Influence of differing carbohydrate sources on L-tryptophan metabolism by porcine fecal microbiota studied in vitro[J].Livestock Science,2009,120(1/2):43-50.

[10] DOERNER K C,COOK K L,MASON B P.3-methylindole production is regulated in Clostridium scatologenes ATCC 25775[J].Letters in Applied Microbiology,2009,48(1):125-132.  

[11] YOKOYAMA M T,JOHNSON K A,CARLSON J R.Factors influencing the production of p-cresol and skatole by Lactobacillus isolated from the rumen and pig feces//Proceedings of 17 conference on rumen function.Chicag:USA,1983:19-20.

[12] JENSEN B B.Prevention of boar taint in pig production.Factors affecting the level of skatole[J].Acta Veterinaria Scandinavica,2006,48(Suppl.1):S6.

[13] PEDERSEN J K,MORTENSEN A B,MADSEN A,et al.Foderets indflydelse pa ornelugt i svinekod statens hysdyrbrugsforsog[J].Meddelse,1986(638):1-7.

[14] RAFFAEL W,ULRIKE W.Nutritional influences on skatole formation and skatole metabolism in the pig[J].Animals,2012,2(2):221-242.

[15] LIN R S,ORCUTT M W,ALLRICH R D,et al.Effect of dietary crude protein content on skatole concentration in boar serum[J].Meat Science,1992,31(4):473-479.  

[16] LUNDSTRÖMA K,MALMFORSB B,STERNB S,et al.Skatole levels in pigs selected for high lean tissue growth rate on different dietary protein levels[J].Livestock Production Science,1994,38(2):125-132.  

[17] AN C,KUDA T,YAZAKI T,et al.Caecal fermentation,putrefaction and microbiotas in rats fed milk casein,soy protein or fish meal[J].Applied Microbiology and Biotechnology,2014,98(6):2779-2787.  

[18] RUSSELL W R,DUNCAN S H,SCOBBIE L,et al.Major phenylpropanoid-derived metabolites in the human gut can arise from microbial fermentation of protein[J].Molecular Nutrition & Food Research,2013,57(3):523-535.  

[19] XU X,HU C,WANG M.Effects of fructooligosaccharide on conversion of L-tryptophan to skatole and indole by mixed populations of pig fecal bacteria[J].The Journal of General and Applied Microbiology,2002,48(2):83-89.  

[20] VHILE S G,KJOS N P,SØ RUM H,et al.Feeding Jerusalem artichoke reduced skatole level and changed intestinal microbiota in the gut of entire male pigs[J].Animal,2012,6(5):807-814.  

[21] LI C Y,WU C,LIU J X,et al.Spatial variation of intestinal skatole production and microbial community in Jinhua and Landrace pigs[J].Journal of the Science of Food and Agriculture,2009,89(4):639-644.  

[22] PASCAL P,HUBERT B,ANNE-MARIE P,et al.Dynamics of a pig slurry microbial community during anaerobic storage and management[J].Applied and Environmental Microbiology,2006,72(5):3578-3585.  

[23] OLSON A B,SIBLEY C D,SCHMIDT L,et al.Development of real-time PCR assays for detection of the Streptococcus milleri group from cystic fibrosis clinical specimens by targeting the cpn60 and 16S rRNA genes[J].Journal of Clinical Microbiology,2010,48(4):1150-1160.  

[24] RINKINEN M L,KOORT J M,OUWEHAND A C,et al.Streptococcus alactolyticus is the dominating culturable lactic acid bacterium species in canine jejunum and feces of four fistulated dogs[J].FEMS Microbiology Letters,2004,230(1):35-39.  

[25] VANDAMME P,DEVRIESE L A,HAESEBROUCK F,et al.Streptococcus intestinalis Robinson et al.,1988 and Streptococcus alactolyticus Farrow et al.,1984 are phenotypically indistinguishable[J].International Journal of Systematic Bacteriology,1999(49):737–741.

[26] Ø VERLANDA M,KJOSB N P,BORGC M,et al.Organic acids in diets for entire male pigs:effect on skatole level,microbiota in digesta,and growth performance[J].Livestock Science,2008,115(2/3):169-178.

[27] NOWAK A,LIBUDZISZ Z.Ability of probiotic Lactobacillus casei DN 114001 to bind or/and metabolise heterocyclic aromatic amines in vitro[J].European Journal of Nutrition,2009,48(7):419-427.  

[28] 李凤.公猪膻味物质粪臭素的乳酸菌降解研究[D].博士学位论文.重庆:西南大学,2011,141-142.

[29] KONSTANTINOV S R,AWATI A,SMIDT H,et al.Specific response of a novel and abundant Lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets[J].Applied and Environmental Microbiology,2004,70(7):3821-3830.  

[30] KIM P I,JUNG M Y,CHANG Y H,et al.Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract[J].Applied Microbiology and Biotechnology,2007,74(5):1103-1111.  

[31] MARTINEZ R C,AYNAOU A E,ALBRECHT S,et al.In vitro evaluation of gastrointestinal survival of Lactobacillus amylovorus DSM 16698 alone and combined with galactooligosaccharides,milk and/or Bifidobacterium animalis subsp.lactis Bb-12[J].International Journal of Food Microbiology,2011,149(2):152-158.  

[32] FINAMORE A,ROSELLI M,IMBINTO A,et al.Lactobacillus amylovorus inhibits the TLR4 inflammatory signaling triggered by enterotoxigenic Escherichia coli via modulation of the negative regulators and involvement of TLR2 in intestinal Caco-2 cells and pig explants[J].PLoS One,2014,9(4):e94891.

[33] EOM H J,MOON J S,SEO Y,et al.Heterologous expression and secretion of Lactobacillus amylovorus alpha-amylase in Leuconostoc citreum[J].Biotechnology Letters,2009,31(11):1783-1788.  

[34] MARTINEZ R C,CARDARELLI H R,BORST W,et al.Effect of galactooligosaccharides and Bifidobacterium animalis Bb-12 on growth of Lactobacillus amylovorus DSM 16698,microbial community structure,and metabolite production in an in vitro colonic model set up with human or pig microbiota[J].FEMS Microbiology Ecology,2013,84(1):110-123.  

[35] MENG X,HE Z F,LI H J.Purification and characterization of a novel skatole-degrading protease from Lactobacillus brevis 1.12[J].Food Science and Biotechnology,2013,22(5):1367-1373.
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