This experiment was conducted to study the effects of dietary riboflavin level on laying performance, egg quality, blood biochemical and antioxidant parameters of laying ducks in cage. A total of 504 healthy Fujian Longyan laying ducks with similar body weight were randomly allotted into 7 groups with 6 replicates per group and 12 ducks per replicate. Ducks in the 7 groups were fed experimental diets supplemented with 0 (group Ⅰ), 3 (group Ⅱ), 6 (group Ⅲ), 9 (group Ⅳ), 12 (group Ⅴ), 15 (group Ⅵ) and 18 mg/kg (group Ⅶ) riboflavin, respectively. The experiment lasted for 12 weeks. The results showed as follows: 1) dietary riboflavin level did not significant affect the laying performance of laying ducks (P>0.05), but the laying rate, average egg weight, daily egg weight and the ratio of feed to egg got the best effect in group Ⅲ. 2) The albumen height in group Ⅲ was significantly higher than that in other groups except group Ⅱ (P<0.05), the Haugh unit in group Ⅲ was significantly higher than that in groups Ⅰ, Ⅴ and Ⅵ (P<0.05). The yolk color was showed an increased tend with the increasing of dietary riboflavin level (P=0.08). 3) Dietary riboflavin level significantly affect blood T lymphocyte proliferation rate (P<0.05), with the increased of dietary riboflavin level, the plasma riboflavin content firstly increased and then decreased, and the erythrocyte glutathione reductase activity coefficient firstly decreased and then increased. 4) Dietary riboflavin level significant affect liver and plasma total superoxide dismutase activity and liver xanthine oxidase activity (P<0.05). In conclusion, the laying ducks can get better performance and egg quality when dietary supplemental level of riboflavin is 6 mg/kg. The antioxidant function of the laying ducks was improved with the increasing of dietary riboflavin level, and the supplemental level of riboflavin is suggested more than 12 mg/kg.
WANG Shuang, CHEN Wei, RUAN Dong, WANG Li, ZHENG Chuntian, WANG Shenglin, LIN Yingcai
. Effects of Dietary Riboflavin Level on Laying Performance, Egg Quality, Blood Biochemical and Antioxidant Parameters of Laying Ducks in Cage[J]. Chinese Journal of Animal Nutrition, 2014
, 26(11)
: 3284
-3290
.
DOI: 10.3969/j.issn.1006-267x.2014.11.014
[1] RIVLIN R S,PINTO J T.Riboflavin (vitamin B2)[M].3rd ed.New York:Marcel Dekker,2001:255-273.
[2] 王鑫,王宝维,葛文华,等.维生素B2对5-16周龄五龙鹅屠宰性能,肌肉品质及脂肪代谢的影响[J].动物营养学报,2014,26(1): 98-105.
[3] 蒋守群,周桂莲,林映才,等.饲粮维生素B2水平对1-21日龄黄羽肉鸡生长性能、免疫机能和抗氧化能力的影响[C]//中国家禽科学研究进展——第十四次全国家禽科学学术讨论会论文集.北京:中国农业科学技术出版社,2009.
[4] 唐静,谢明,闻治国,等.核黄素对北京鸭生长性能和抗氧化机能的影响[J].动物营养学报,2013,25(12):2883-2887.
[5] LEVIN G,COGAN U,LEVY Y,et al.Riboflavin deficiency and the function and fluidity of rat erythrocyte membranes[J]. The Journal of Nutrition,1990,120(8):857-861.
[6] NICHOALDS G.Assessment of status of riboflavin nutriture by assay of erythrocyte glutathione reductase activity[J]. Clinical Chemistry,1974,20(5):624-628.
[7] 韦京豫, 郭长江, 杨继军,等.高效液相色谱法测定大鼠血浆和全血中核黄素的含量[J].色谱,2003,21(4):375-377.
[8] 霍思远,王安,冯婧.核黄素对笼养生长期蛋鸭生产性能,激素分泌及免疫器官发育的影响[J].动物营养学报,2011,23(11):1906-1911.
[9] 陈雷,郭海燕,安军,等.维生素B2对蛋鸡产蛋性能和鸡蛋中维生素B2含量影响[J].中国饲料,2010,16:11.
[10] 任转龙,龚月生,武会娟.维生素A、E、B2对蛋鸡生产性能及生化指标的影响[J].饲料与畜牧,2007(5):44-46.
[11] 陈艳珍.蛋鸡日粮中添加核黄素的效果研究[J].中国饲料,2004(16):16-17.
[12] 赵凯,李太平.在蛋种鸡和肉种鸡日粮中添加核黄素的效果[J].当代畜牧,1996(6):11,16.
[13] 李小利.哈氏单位是检验鸡蛋品质的重要指标[J].检验检疫学刊,2013(2):48-49.
[14] 尹兆正,童莲芳.蛋黄色素的研究进展[J].中国家禽,1994(4):32-34.
[15] 王景成,周佳萍.蛋黄颜色的着色机理及改善措施[J].饲料博览:技术版,2008(6):17-18.
[16] 郑诚,董泽敏.抗氧化剂对饲粮中叶黄素保护作用效果的研究[J].广东饲料,1997(1):20-21.
[17] FRANK G,BAHR J,EASTER R.Riboflavin requirement of lactating swine[J].Journal of Animal Science,1988,66(1):47-52.
[18] FRANK G,BAHR J,EASTER R.Riboflavin requirement of gestating swine[J].Journal of Animal Science,1984,59(6):1567-1572.
[19] 蒋守群,周桂莲,林映才,等.饲粮维生素E水平对22-42日龄黄羽肉鸡生长性能、免疫功能和抗氧化能力的影响[J].动物营养学报,2013,25(2):289-298.
[20] 梁惠芳,柳启沛.核黄素对大鼠脂质过氧化影响的研究[J].卫生研究,1999,28(6):370-371.
[21] 张建海,原广华.不同核黄素水平对肉仔鸡外用血细胞、免疫器官及生产性能的影响[J].山东家禽,2003(2):9-11.
[22] 任延铭,芦燕,王安.低温环境下饲粮核黄素添加水平对蛋鸭生长发育、免疫器官及抗氧化功能的影响[J].动物营养学报,2011,23(11):1912-1918.
[23] 王艳辉,王安,谢富.维生素B2对笼养蛋雏鸭生长性能内分泌及抗氧化能力的影响[J].动物营养学报,2009,21(1):31-35.
[24] CHRISTENSEN H N.Riboflavin can protect tissues from oxidative injury[J].Nutrition Reviews,1993,51(5):149.
