Comparative Study on Test Methods for Antibacterial Activities of Antibacterial Peptides from Spleen of Japanese Eel (Anguilla japonica)

LIANG Ying, HUANG Wenshu, GUAN Ruizhang

doi:10.3969/j.issn.1006-267x.2014.11.036

2014 , Vol. 26 >Issue 11: 3461 - 3468

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.036

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Comparative Study on Test Methods for Antibacterial Activities of Antibacterial Peptides from Spleen of Japanese Eel (Anguilla japonica)

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1. Fisheries College, Jimei University, Xiamen 361021, China;
2. Engineering Research Centre of Eel Modern Technical Industry, Ministry of Education, Jimei University, Xiamen 361021, China

Received date: 2014-05-13

Online published: 2014-11-17

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Abstract

The study was to establish a simple and sensitive test method for antibacterial activity analysis in the separation and purification of antibacterial peptides from the spleen of Japanese eel (Anguilla japonica). Experiment by using spleen protein of molecular mass<10 ku from Japanese eel as antibacterial peptide samples, to compare the sensitivity, advantages and disadvantages of three test methods. In these three test methods, two conventional methods were agar plate fovea diffusion method and microporous liquid culture method and another was trace liquid culture method, for testing antibacterial activities of antibacterial peptides against three common pathogenic bacterial strains of eel, including Edwardsiella tarda, Aeromonas sp. and Aeromonas hydrophila. The results showed that compared with the agar plate fovea diffusion method and microporous liquid culture method, the trace liquid culture method had the advantages of simple operation and easy to observe result. Furthermore, this method with the best sensitivity and using the least sample quantity could be suitable for testing antibacterial activities in the separation and purification of antibacterial peptides from the spleen of eel, especially for identifying antibacterial activity of the a few and single fraction obtained in late stage of separation and purification. This study suggests that the trace liquid culture method is suitable for testing antibacterial activities of antibacterial peptides from the spleen of Japanese eel.

Key words： Japanese eel (Anguilla japonica); spleen; antibacterial peptides; antibacterial activity

Cite this article

LIANG Ying, HUANG Wenshu, GUAN Ruizhang . Comparative Study on Test Methods for Antibacterial Activities of Antibacterial Peptides from Spleen of Japanese Eel (Anguilla japonica)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3461 -3468 . DOI: 10.3969/j.issn.1006-267x.2014.11.036

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