Aquaculture Nutrition

Effects of Peptides from Swine Blood on Antioxidant Capacity and Immune Function of Jian Carp (Cyprinus carpio var. Jian)

  • WANG Jiahao ,
  • JIA Rui ,
  • LIU Yingjuan ,
  • DU Jinliang ,
  • CAO Liping ,
  • YIN Guojun
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  • 1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;
    2. Key Laboratory of Gentic Breeding and Aquaculture Biology of Freshwater Fishes of Ministry of Agriculture/International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

Received date: 2014-06-05

  Online published: 2014-12-05

Abstract

This experiment was conducted to study the effects of peptides from swine blood (PSB) on antioxidant capacity and immune function of Jian carp (Cyprinus carpio var. Jian) at in vitro and in vivo levels. In vitro experiment, the head-kidney macrophages and peripheral blood leukocytes, isolated from Jian carp, were cultured and treated for 24 hours with different concentrations (0, 10, 20, 40 and 80 μg/mL) of PSB. To evaluate the immunostimulating effects of PSB, the viability of peripheral blood leukocytes was measured by WST-1 method, and the respiratory burst activities of oxygen and nitrogen of head kidney macrophages were investigated by nitroblue tetrazolium (NBT) reduction method and Griess reagent colorimetric method, respectively. In vivo experiment, PSB was added to the basal diet at the concentrations of 0, 0.5, 1.0, 2.0 and 5.0 g/kg, fed to Jian carp in constant temperature cycle water system for 8 weeks. The blood samples were collected four times around the time points of the 1st week, the 2nd week, the 4th week and the 8th week, then detected the changes of serum biochemical indices, antioxidant indices and immune indices. The results showed as follows: in vitro experiment, the PSB at the concentrations of 20, 40 and 80 μg/mL could significantly enhance the viability of peripheral blood leucocytes (P<0.05 or P<0.01); PSB at the concentrations of 40 and 80 μg/mL could significantly enhance the respiratory burst activities of oxygen and nitrogen (P<0.05 or P<0.01). In vivo experiment, after Jian carp were fed the diet with PSB at the concentration of 5.0 g/kg, the contents/activity of 3 biochemical indices [albumin (ALB), total protein (TP) and alkaline phosphatase (AKP)], the activities of 3 antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)] and the contents of 3 immune factors [interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and complement 4 (C4)] in serum at the 4th week and the 8th week were significantly increased (P<0.05); moreover, the content of complement 4 (C4) in serum at the 8th week was significantly increased aslo (P<0.05). Therefor, the PSB can improve the antioxidant capacity and immune function of Jian carp.

Cite this article

WANG Jiahao , JIA Rui , LIU Yingjuan , DU Jinliang , CAO Liping , YIN Guojun . Effects of Peptides from Swine Blood on Antioxidant Capacity and Immune Function of Jian Carp (Cyprinus carpio var. Jian)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(12) : 3689 -3697 . DOI: 10.3969/j.issn.1006-267x.2014.12.019

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