Effects of n-3 Fatty Acids with Different Carbon Chain Lengths and Lipid Levels on Growth, Liver Function and Postprandial Blood Indices of Tilapia (Oreochromis niloticus)

LIN Xin, LI Fajian, LIN Shimei, PENG Xianghe, ZHU Wangming

doi:10.3969/j.issn.1006-267x.2015.03.015

2015 , Vol. 27 >Issue 3: 775 - 784

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.03.015

Aquaculture Nutrition

Effects of n-3 Fatty Acids with Different Carbon Chain Lengths and Lipid Levels on Growth, Liver Function and Postprandial Blood Indices of Tilapia (Oreochromis niloticus)

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1. College of Animal Science and Technology, Southwest University, Chongqing 400716, China;
2. Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Chongqing 400716, China;
3. Guangzhou Sintun Aqua-Tech Co., Ltd., Guangzhou 510642, China

Received date: 2014-09-02

Online published: 2015-03-18

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Abstract

An 8-week feeding trial was conducted to evaluate the effects of n-3 fatty acids with different carbon chain lengths and lipid levels on growth, liver function and postprandial blood indices of tilapia (Oreochromis niloticus). Four experimental diets were formulated containing either 5% fish oil (low level of fish oil), 10% fish oil (high level of fish oil), 5% linseed oil (low level of linseed oil) or 10% linseed oil (high level of linseed oil) on the basis of practical diet formulation. A total of 360 tilapia with the initial body weight of (43.70±1.06) g were randomly distributed into 4 groups with 3 replicates per group and 30 fish per replicate, and the fish in each group were randomly fed one of four experimental diets. The results showed that no significant differences were found in finial body weight (FBW), specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) of fish among all groups (P>0.05), but the feed intake (FI) in low level of linseed oil group was significantly lower than that in high level of linseed oil group (P<0.05). Serum total cholesterol (TCHO), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) contents in fish oil groups were higher than those in linseed oil groups; besides, the above indices in high level group were higher than those in low level group of the same lipid source. The peak of serum TCHO content in fish oil groups was observed at 6 h after meal, but that in linseed oil groups was observed at 9 h after meal. The bottom of serum HDL-C content appeared at 6 h after meal in 4 groups. Linseed oil groups had higher serum triglyceride (TG) and very low density lipoprotein-cholesterol (VLDL-C) contents compared with fish oil groups. With the postprandial time going on, blood glucose content showed a basic decreasing tendency. The hepatopancreas alanine transaminase (ALT) activity in fish oil groups was significantly lower than that in linseed oil groups (P<0.01), but there were no differences in hepatopancreas aspartate aminotransferase (AST) activity among all groups (P>0.05). Hepatopancreas malondialdehyde (MDA) content showed a trend of high level of fish oil group>high level of linseed oil group>low level of fish oil group>low level of linseed oil group, and the difference among all groups was significant (P<0.01). No difference was found in hepatopancreas superoxide dismutase (SOD) activity between fish oil and linseed oil groups in the same level (P>0.05), but the hepatopancreas SOD activity in low level of fish oil group was significantly higher than that in high level of fish oil group (P<0.05). In conclusion, n-3 fatty acids with different carbon chain lengths and lipid levels have little effects on growth of tilapia, but can affect their liver function and postprandial blood indices.

Key words： tilapia (Oreochromis niloticus); fish oil; linseed oil; growth; liver function; postprandial blood indices

Cite this article

LIN Xin, LI Fajian, LIN Shimei, PENG Xianghe, ZHU Wangming . Effects of n-3 Fatty Acids with Different Carbon Chain Lengths and Lipid Levels on Growth, Liver Function and Postprandial Blood Indices of Tilapia (Oreochromis niloticus)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(3) : 775 -784 . DOI: 10.3969/j.issn.1006-267x.2015.03.015

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