Short Communications

Effects of Formic Acid, Cellulose and Lactic Acid Bacteria on Silage Quality of Natural Forage of Typical Steppe

  • HOU Meiling ,
  • GE Gentu ,
  • SUN Lin ,
  • ZHOU Tianrong ,
  • ZHANG Yingchao ,
  • JIA Yushan
Expand
  • College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot 010019, China

Received date: 2015-03-27

  Online published: 2015-09-12

Abstract

This experiment was conducted to selected the suitable supplemental concentrations of formic acid (FA), cellulose (CE) and lactic acid bacteria (LAB) for natural grass silage, and to provide a scientific basis for forage grass silage additives. A single factor completely randomized was uesd in the experimen, added FA (3.00, 6.00 and 9.00 mL/kg), CE (0.05, 0.10 and 0.15 g/kg) and LAB (0.01, 0.02 and 0.03 g/kg) of 3 different kinds of additives in natural grass forage, and the control group (CK) was without any addition. Analyzed the sensory quality, fermentation quality, nutrient composition and in vitro digestibility of natural forge silage nder different additives. The results showed as follows: 1) added 6.0 mL/kg FA group had been significantly improved the sensory quality, the pH, ammoniacal nitrogen/total nitrogen (AN/TN) were significantly lower than CK (P0.05), the in vitro digestibility of gross energy (GE) and digestive energy (DE) was significantly higher than CK (P0.05). 2) The sensory quality of added 0.15 g/kg CE group had been significantly better than the control group, the pH, AN/TN were significantly lower than CK (P<0.05), the contents of DM, CP, ether extract (EE) and WSC were significantly higher than CK (P<0.05), the contents of NDF and ADF were significantly lower than CK (P<0.05), the GE, DE and IVDMD were significantly higher than CK (P<0.05). 3) The sensory quality of added 0.02 g/kg LAB group had been significantly improved, the pH, AN/TN were significantly lower than CK (P<0.05), the lactic acid content was significantly higher than CK (P<0.05), the contents of DM, CP, EE were significantly higher than CK (P<0.05), the content of NDF and ADF was significantly lower than CK (P<0.05), the GE, DE and IVDMD were significantly higher than CK (P<0.05). According to principal component analysis, added 6.0 mL/kg FA, 0.1g/kg CE or 0.02 g/kg LAB, the natural grass silage fermentation can guarantee good quality, and can effectively save the nutritional value of forage.

Cite this article

HOU Meiling , GE Gentu , SUN Lin , ZHOU Tianrong , ZHANG Yingchao , JIA Yushan . Effects of Formic Acid, Cellulose and Lactic Acid Bacteria on Silage Quality of Natural Forage of Typical Steppe[J]. Chinese Journal of Animal Nutrition, 2015 , 27(9) : 2977 -2986 . DOI: 10.3969/j.issn.1006-267x.2015.09.039

References

[1] 郝文强.内蒙古草原牧区可持续发展制度建设研究[D].硕士学位论文.呼和浩特:内蒙古大学,2014:1-3.
[2] 李海,贾玉山,格根图,等.处理方式对天然牧草青贮料有机酸的影响[J].草地学报,2008,16(2):154-157.
[3] 周治云,李昌桂.牧草高效生产与加工技术[M].北京:中国农业大学出版社,1996:23-53.
[4] NGWA T A,NSAHLAI I V,IJI P A.Ensilage as a means of reducing the concentration of cyanogenic glycosides in the pods of Acacia sieberiana and the effect of additives on silage quality[J].Journal of the Science of Food and Agriculture,2004,84(6):521-529.  
[5] 庄益芬,安宅一夫,张文昌.不同温度条件下添加剂对青贮苜蓿细胞壁成分和体外干物质消化率的影响[J].家畜生态学报,2006,27(6):61-64.
[6] 薛艳林,孙启忠,赵和平,等.沙蒿与玉米秸秆混合比例和乳酸菌制剂对混合青贮饲料品质的影响[J].动物营养学报,2014,26(5):1310-1319.
[7] 张晓庆,李鹏,郑琛,等.添加甲酸对麻叶荨麻青贮品质的影响[J].草地学报,2013,21(3):618-621,625.
[8] DEAN D B,ADESONGEN A T,KRUEGER N,et al.Effect of fibrolytic enzymes on the fermentation characteristics,aerobic stability,and digestibility of Bermuda grass silage[J].Journal of Dairy Science,2005,88(3):994-1003.  
[9] 马清河,李茂,周汉林.纤维素酶对王草青贮品质和碳水化合物含量的影响[J].黑龙江畜牧兽医,2011(2):79-81.
[10] 李军训,高洁,王建华,等.青贮乳酸菌的发酵生物量的研究[J].饲料工业,2004,25(7):48-51.
[11] 祁永.放牧对草原群落特征及种群繁殖特性的影响[D].博士学位论文.北京:中国农业大学,2005:47-85.
[12] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2004:99-110.
[13] BRODERICA G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.  
[14] 郭望山,孟庆翔.杜马斯燃烧法与凯氏法测定饲料含氮量的比较研究[J].畜牧兽医学报,2006,37(5):464-468.
[15] 杨胜.饲料分析及饲料质量检测技术[M].北京:北京农业大学出版社,1993:15-98.
[16] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstareh polysaecharides in relation to animal nutrition[J].Journal of Dairy Scienee,1991,74(10):3583-3597.  
[17] PLAYNE M J,MCDONALD P.The buffering constituents of herbage and of silage[J].Journal of the Science of Food and Agriculture,1966,17(6):264-268.  
[18] 王鸿泽,彭全辉,康坤,等.不同混合比例对甘薯蔓、酒糟及稻草混合青贮品质的影响[J].动物营养学报,2014,26(12):3868-3876.
[19] 内蒙古自治县质量技术监督局.DB15/T 30—92.细毛羊饲养标准[S].呼和浩特:内蒙古自治县质量技术监督局,2009.
[20] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen flued[J].Animal Research and Development,1988,28:47-55.
[21] 荣磊.苜蓿草捆贮藏品质调控方法研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2013.
[22] PATTERSON H H,ADAMS D C,KLOPFENSTEIN T J,et al.Supplementation to meet metabolizable protein requirements of primiparous beef heifers:Ⅱ.Pregnancy and ecomomics[J].Journal of Animal Science,2003,81(3):564-570.
[23] 荣辉,陈杰,余成群,等.添加甲酸对象草青贮发酵品质的影响[J].草地学报,2012,20(6):1106-1111.
[24] PHILLIP L E,UNDERHILL L,GARINO H.Effects of treating lucerne with an inoculum of lactic acid bacteria or formic acid upon chemical changes during fermentation,and upon the nutritive value of the silage for lambs[J].Grass and Forage Science,1990,45(3):337-344.  
[25] CHAMBERLAIN D G,THOMAS P C,WAIT M K.The rate of addition of formic acid to grass at ensilage and the subsequent digestion of the silage in the rumen and intestine of sheep[J].Grass and Forage Science,1982,37(2):159-164.  
[26] 郭金双.不同青贮添加剂对青贮品质的影响[D].硕士学位论文.北京:中国农业大学,1998:42-79.
[27] CARPIRERO C M,HENDERSON A R,MCDONALD P.The effect of some pre-treatments on proteolysis during the ensiling of herbage[J].Grass and Forage Science,1979,34(4):311-315.  
[28] KENNEDY S J.Comparison of the fermentation quality and nutritive value of sulphuric and formic acid-treated silages fed to beef cattle[J].Grass and Forage Science,1990,45(1):17-28.  
[29] 李清宏,高文俊,王永新,等.添加剂及晾晒对多变小冠花青贮影响的研究[J].草地学报,2012,20(2):363-367.
[30] 王奇,余成群,李志华,等.添加酶和乳酸菌制剂对西藏苇状羊茅和箭筈豌豆混合青贮发酵品质的影响[J].草业学报,2012,21(4):186-191.
[31] SINGH K,HONIG H,WERMKE M,et al.Fermentation pattern and changes in cell wall constituents of straw-forage silages,straws and partners during storage[J].Animal Feed Science and Technology,1996,61(1/2/3/4):137-153.
[32] JAAKKOLA S,HUHTANEN P,HISSA K.The effect of cell wall degrading enzymes or formic acid on fermentation quality and on digestion of grass silage by cattle[J].Grass and Forage Science,1991,46(1):75-87.  
[33] 贾燕霞,玉柱,邵涛.添加酶制剂对象草青贮发酵品质的影响[J].草地学报,2009,17(1):121-124.
[34] HRISTOV A N.Effect of a commercial enzyme preparation on alfalfa silage fermentation and protein degradability[J].Animal Feed Science and Technology,1993,42(3/4):273-282.
[35] HENDERSON A R,MCDONALD P.The effect of cellulase preparations on the chemical changes during the ensilage of grass in laboratory silos[J].Journal of the Science of Food and Agriculture,1977,28(6):486-490.  
[36] HOFFMAN P C,WELCH D A,BREHM N M.Potential of enzyme mixtures to improve silage quality and lactation performance of dairy cattle[J].Journal of Production Agriculture,1995,8(4):552-557.  
[37] 张静,玉柱,邵涛.丙酸、乳酸菌添加剂对多花黑麦草青贮发酵品质的影响[J].草地学报,2009,17(2):162-165.
[38] 王昆昆,玉柱,邵涛,等.乳酸菌制剂对不同比例苜蓿和披碱草混贮发酵品质的影响[J].草业学报,2010,19(4):94-100.
[39] 管武太,ASHBELL G,HEN Y,等.微生物添加剂对青贮高粱发酵品质和稳定性的影响[J].中国农业科学,2002,35(11):1401-1405.
[40] 陈兴,茅慧玲,王佳堃,等.外源纤维素酶制剂对青贮玉米体外发酵特性以及甲烷生成的影响[J].动物营养学报,2014,25(1):214-221.
[41] 张涛.紫花苜蓿青贮菌剂筛选、作用机理及其应用研究[D].博士学位论文.北京:中国农业大学,2005:32-56.
[42] 李福昌,王中华,杨维仁,等.在玉米秸青贮料中添加不同的青贮添加剂对肉牛的饲喂效果[J].饲料研究,2001(6):34-35.
Outlines

/