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Effects of Glutamine and Its Precursors on Tissue Antioxidant Capacity and Serum Biochemical Indices of Songpu Mirror Carp

  • SONG Fangjie ,
  • WANG Liansheng ,
  • XU Qiyou
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  • 1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214182, China;
    2. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China

Received date: 2015-08-23

  Online published: 2016-02-19

Abstract

This experiment was conducted to study the effects of glutamine (Gln) and its precursors on tissue antioxidant capacity and serum biochemical indices of Songpu mirror carp. A total of 1 050 Songpu mirror carp with an average body weight of (40.27±3.96) g were randomly selected and divided into 7 groups with 5 replicates per group, and 30 fish in each replicate. The fish in those groups were fed 7 isonitrogenous and isocaloric diets with 1.5% glucose (control), glutamine (Gln), glutamate (Glu), alpha-ketoglutarate (AKG), ornithine-alpha-ketoglutarate (OKG), arginine-alpha-ketoglutarate (AAKG) and sodium alpha-ketoglutarate dibasic (2Na-AKG), respectively. The feeding trial lasted for 8 weeks. The results showed as follows:the superoxide dismutase (SOD) activity of the Gln and Glu groups was significantly higher than that of control group (P<0.05), the content of glutathione (GSH) of the Gln group was significantly higher than that of control group (P<0.05), the content of malondialdehyde (MDA) of the Glu, AKG, OKG and 2Na-AKG groups was significantly lower than that of control group (P<0.05) in intestine; the SOD activity of the Gln and Glu groups was significantly higher than that of other groups (P<0.05), the content of GSH had no significant difference among the groups (P>0.05), the content of MDA of the Gln, Glu, AKG, 2Na-AKG and OKG groups was significantly lower than that of control group (P<0.05) in liver; the SOD activity of the OKG and 2Na-AKG groups was significantly higher than that of control group (P<0.05), the content of GSH of the 2Na-AKG group was significantly lower than that of control group (P<0.05), the content of MDA had no significant difference among the groups (P>0.05) in serum. Compared with the control group, the serum TP content of the Gln, Glu and AKG groups was significantly increased (P<0.05), and that of 2Na-AKG group was significantly decreased (P<0.05); the serum alanine transarninase (ALT) activity of the AAKG group was significantly increased (P<0.05), the serum aspartate aminotransferase (AST) activity of the OKG and 2Na-AKG groups was significantly increased (P<0.05) and that of the AKG group was significantly decreased (P<0.05); the serum total cholesterol (TCHO) content of the AAKG group was significantly decreased (P<0.05); the serum glucose content of the AKG group was significantly decreased (P<0.05). In conclusion, Gln and its precursors have some effect on tissue antioxidant capacity, and AKG, OKG, 2Na-AKG are better than others on removal of lipid peroxidation products; Gln, Glu and AKG can improve the protein utilization and immunity, moreover, AKG can reduce the blood glucose content.

Cite this article

SONG Fangjie , WANG Liansheng , XU Qiyou . Effects of Glutamine and Its Precursors on Tissue Antioxidant Capacity and Serum Biochemical Indices of Songpu Mirror Carp[J]. Chinese Journal of Animal Nutrition, 2016 , 28(2) : 627 -634 . DOI: 10.3969/j.issn.1006-267x.2016.02.039

References

[1] WU G,KNABE D A,FLYNN N E.Synthesis of citrulline from glutamine in pig enterocytes[J].The Biochemical Journal,1994,299(Pt 1):115-121.
[2] KREBS H A,BAVEREL G,LUND P.Effect of bicarbonate on glutamine metabolism[J].The International Journal of Biochemistry,1980,12(1/2):69-73.
[3] NEWSHOLME E A,CARRIÉ A L.Quantitative aspects of glucose and glutamine metabolism by intestinal cells[J].Gut,1994,35(1 Suppl.):S13-S17.
[4] 白由元,于明琨,刘震洋.谷氨酰胺与肠道免疫调节作用[J].中国临床康复,2006,10(4):153-155.
[5] LIN Y,ZHOU X Q.Dietary glutamine supplementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture,2006,256(1/2/3/4):389-394.
[6] CHEN J,ZHOU X Q,FENG L,et al.Effects of glutamine on hydrogen peroxide-induced oxidative damage in intestinal epithelial cells of Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture,2009,288(3/4):285-289.
[7] 徐奇友,王常安,许红,等.外源性谷氨酰胺对虹鳟稚鱼生长和肠道形态的影响[J].中国粮油学报,2009,24(4):98-102.
[8] BRITO G A C,CARNEIRO-FILHO B A,ORIÁ R B,et al.Clostridium difficile toxin A induces intestinal epithelial cell apoptosis and damage:role of Gln and Ala-Gln in toxin A effects[J].Digestive Diseases and Sciences,2005,50(7):1271-1278.  
[9] KIM S J, KOO O J,KWON D K,et al.Replacement of glutamine with the dipeptide derivative alanyl-glutamine enhances in vitro maturation of porcine oocytes and development of embryos[J].Zygote,2013,22(2):186-289.
[10] 李晋南,徐奇友,位莹莹,等.谷氨酰胺及其前体物对松浦镜鲤生长性能、体成分和血清生化指标的影响[J].东北农业大学学报,2013,44(12):119-125.
[11] 李晋南,徐奇友,王常安,等.谷氨酰胺及其前体物对松浦镜鲤肠道消化酶活性及肠道形态的影响[J].动物营养学报,2014,26(5):1347-1352.
[12] 魏玉强,徐奇友,位莹莹,等.不同蛋白源饲料中添加α-酮戊二酸对松浦镜鲤肌肉成分、血清氨基酸和生化指标的影响[J].东北农业大学学报,2015,46(1):94-100.
[13] 陈瑾,周小秋,冯琳.谷氨酰胺对动物肠道抗氧化能力的影响[J].饲料工业,2009,30(2):55-57.
[14] 陈瑾.谷氨酰胺对鲤鱼肠上皮细胞抗氧化能力的影响[D].硕士学位论文.雅安:四川农业大学,2008.
[15] 刘坚,侯永清,丁斌鹰,等.α-酮戊二酸对脂多糖应激断奶仔猪空肠黏膜蛋白合成和抗氧化能力的影响[J].营养饲料,2010,46(11):35-38.
[16] 曹锡清.脂质过氧化对细胞与机体的作用[J].生物化学与生物物理进展,1986(2):17-22,23.
[17] 程时,丁海勤.谷胱甘肽及其抗氧化作用今日谈[J].生理科学进展,2002,33(1):85-90.
[18] 李华涛.谷氨酰胺抗鲤鱼红细胞凋亡作用及作用机制研究[D].博士学位论文.雅安:四川农业大学,2013.
[19] 夏晶.Gln对嗜水气单胞菌感染中华鳖的保护作用[D].硕士学位论文.雅安:四川农业大学,2012.
[20] VELVIZHI S,DAKSHAYANI K B,SUBRAMANIAN P.Effects of α-ketoglutarate on antioxidants and lipid peroxidation products in rats treated with ammonium acetate[J]. The Journal of Nutrition,2002,18(9):747-750.  
[21] 张军民,王连递,高振川,等.日粮添加谷氨酰胺对早期断奶仔猪抗氧化能力的影响[J].畜牧兽医学报,2002,33(2):105-109.
[22] 黄冠庆,林红英.丙氨酰谷氨酰胺对断奶仔猪生长性能及血清生化指标的影响[J].中国农学通报,2009,25(2):9-12.
[23] 徐涛,王荣延.临床检验基础[M].北京:人民卫生出版社,1983:165-168.
[24] 褚武英,吴信,成嘉,等.低聚木糖对草鱼生长性能及血液生化指标的影响[J].饲料研究,2008(6):60-61.
[25] 王爱民,韩光明,封功能,等.饲料脂肪水平对吉富罗非鱼生产性能、营养物质消化及血液生化指标的影响[J].水生生物学报,2011,35(1):80-87.
[26] 朱青,许红,徐奇友,等.谷氨酰胺对幼鲟鱼血清、肝胰脏生化指标及体成分的影响[J].水产学杂志,2010,23(2):16-20.
[27] 陈玉春,顾雪飞,刘敏.5种中草药对鲤血清谷丙转氨酶、谷草转氨酶及红细胞过氧化氢酶活性的影响[J].淡水渔业,2007,37(5):11-13.
[28] 惠天朝,施明华,朱荫湄.硒对罗非鱼慢性镉中毒肝抗氧化酶及转氨酶的影响[J].中国兽医学报,2000,20(3):264-266.
[29] WILSON R P,POE W E.Apparent inability of channel catfish to utilize dietary mono- and disaccharides as energy sources[J].The Journal of Nutrition,1987,117(2):280-285.
[30] BRAUGE C,CORRAZE G,MÉDALE F.Effect of dietary levels of lipid and carbohydrate on growth performance,body composition,nitrogen excretion and plasma glucose levels in rainbow trout reared at 8 or 18℃[J].Reproduction Nutrition Development,1995,35(3):277-290.  
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