Effects of Flavonoids from Allium mongolicum Regel on Antioxidant Capacity of Meat Sheep

MU Qier; AO Changjin; SA Ruli; WANG Terigele; CHEN Renwei; TE Muqile; WANG Cuifang

doi:10.3969/j.issn.1006-267x.2016.06.025

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 6: 1823 - 1831

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.06.025

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Effects of Flavonoids from Allium mongolicum Regel on Antioxidant Capacity of Meat Sheep

MU Qier ,
AO Changjin ,
SA Ruli ,
WANG Terigele ,
CHEN Renwei ,
TE Muqile ,
WANG Cuifang

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2016-03-24

Online published: 2016-06-27

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Abstract

This experiment was conducted to investigate the effects of dietary supplementation of flavonoids from Allium mongolicum Regel on total antioxidant capacity (T-AOC), the activities of total superoxide dismutase (T-SOD), catalase (CAT) and glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) content of meat sheep to determine the optimal level. Sixty 6-month-old meat sheep with similar body weight [(39.9±3.2) kg] were randomly assigned to 4 groups with 15 sheep per group. Control group was fed a basal diet, and the 3 experimental groups were fed the basal diet supplemented with 11, 22 and 33 mg/kg flavonoids from Allium mongolicum Regel, respectively. Preliminary feeding period was 15 d, and the experimental period was 60 d. Blood was collected at days 0, 15, 30, 45, 60 via jugular venipuncture, and serum was harvested. Three sheep were randomly selected from each of the four groups for slaughter after experiment, and samples of liver and spleen were collected to determine the antioxidant indices. The results showed as follows: 1) dietary supplementation of 11 to 33 mg/kg flavoniods from Allium mongolicum Regel significantly increased T-AOC in serum (from day 45) and liver (P<0.05), but had no significant effect on that in spleen (P>0.05). 2)Dietary supplementation of 11 to 33 mg/kg flavoniods from Allium mongolicum Regel had positive effect on the activities of T-SOD in serum, liver and spleen, CAT in serum, and GSH-PX in serum and spleen; the supplemental level of 33 mg/kg showed the highest effects, and the effects became obvious after day 30 in serum; however, it had no significant effects on CAT in liver and spleen, and GSH-PX in liver (P>0.05). 3) Dietary supplementation of 11 to 33 mg/kg flavoniods from Allium mongolicum Regel could decrease the content of MDA in serum and liver, and the effects became obvious after day 15 in serum, however, it had no significant effects on the content of MDA in spleen (P>0.05). The results indicate that dietary supplementation of 11 to 33 mg/kg flavoniods from Allium mongolicum Regel can significantly improve antioxidant capacity of meat sheep, and the antioxidant capacity is in time-dependent and dose-dependent manners. Flavoniods from Allium mongolicum Regel begin to play its role after fed for 30 d.

Key words： Allium mongolicum Regel; flavoniods; meat sheep; antioxidant capacity

Cite this article

MU Qier , AO Changjin , SA Ruli , WANG Terigele , CHEN Renwei , TE Muqile , WANG Cuifang . Effects of Flavonoids from Allium mongolicum Regel on Antioxidant Capacity of Meat Sheep[J]. Chinese Journal of Animal Nutrition, 2016 , 28(6) : 1823 -1831 . DOI: 10.3969/j.issn.1006-267x.2016.06.025

References

[1] 包萨如拉.内蒙古野生葱属(Allium L.)植物的民族植物学研究[D].硕士学位论文.呼和浩特:内蒙古师范大学,2007.
[2] 张培松,周玉香.影响羊肉风味物质的饲料和营养因素[J].畜牧与饲料科学,2013,34(7/8):62-63.
[3] 杨忠仁,刘建文,郝丽珍,等.内蒙古地区四种葱属野菜花期物候及花营养成分研究[J].北方园艺,2013(8):1-4.
[4] 苗春乐.刈割次数对沙葱生长发育及营养成分含量影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2008.
[5] 斯琴巴特尔,刘新民.蒙古韭的营养成分及民族植物学[J].中国草地,2002,24(3):52-54.
[6] 韩正高,赵萍,王丽君.大葱在体外对Hela细胞株的抗增殖作用[J].营养学报,1996,18(2):203-205.
[7] MIMAKI Y,KURODA M,FUKASAWA T,et al.Steroidal glycosides from the bulbs of Allium jesdianum[J].Journal of Natural Products,1999,62(1):194-197.

[8] 燕玲,王六英,李红.内蒙古10种葱属植物染色体数目及核型分析[J].中国草地学报,1999(5):73-75.
[9] 李亚蕾,罗瑞明.沙葱提取物抗菌效果的研究[J].安徽农业科学,2008,36(33):14596-14597,14618.
[10] 凌关庭.天然抗氧化剂及其消除氧自由基的进展[J].食品工业,2000(3):19-22.
[11] 缪亚娟.沙葱异黄酮含量测定及其对机体抗氧化与非特异性免疫影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2009.
[12] 龙春,高志强,陈凤鸣,等.黄酮类化合物的结构-抗氧化活性关系研究进展[J].重庆文理学院学报:自然科学版,2006,5(2):13-17.
[13] 赵春艳.沙葱中黄酮类化合物的分离纯化、结构鉴定及其对小鼠免疫抗氧化机能影响的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2008.
[14] 张甘良,汪钊,鄢洪德.生物类黄酮化合物的结构与生物活性的关系[J].生物学杂志,2005,22(1):4-7.
[15] 包玲玲.沙葱黄酮类化合物对绵羊瘤胃发酵、内环境参数及纤维素降解的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2015.
[16] 萨茹丽.沙葱黄酮提取工艺优化、结构鉴定及其相关生物活性研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2014.
[17] 宋丽霞,敖长金,蔺婷娟,等.沙葱多糖对肉羊抗氧化能力的影响[J].中国畜牧杂志,2012,48(1):56-58.
[18] SUN Y,OBERLEY L W, LI Y.A simple method for clinical assay of superoxide dismutase[J].Clinical Chemistry,1988,34(3):497-500.
[19] CANBAZ H,AKCA T,Tataroglu C,et al.The effects of exogenous L-carnitine on lipid peroxidation and tissue damage in an experimental warm hepatic ischemia-reperfusion injury model[J].Current Therapeutic Research,2007,68(1):32-46.

[20] 那乌莫娃,成绍鑫.腐植酸分子结构与生物活性的关系[J].腐植酸,2002(1):38-43.
[21] 石娟.黑米中花青素的提取、纯化及其体内抗氧化活性研究[D].硕士学位论文.合肥:合肥工业大学,2012.
[22] 李德生,方正锋,车炼强,等.饲粮添加王不留行与大豆异黄酮对泌乳母猪生产性能和抗氧化能力的影响[J].中国畜牧杂志,2014,50(1):63-68.
[23] 王文君,蔡教英,蒋艳,等.芦荟黄酮体内抗氧化活性及对CAT mRNA表达的影响[J].中国食品学报,2013,13(10):13-18.
[24] 杨现艳.沙棘籽渣黄酮对更年期大鼠血脂异常代谢的影响[D].硕士学位论文.上海:华东师范大学,2006.
[25] 王安银,郝志录,张君,等.胡杨叶总黄酮提取物的体内抗氧化活性研究[J].新疆农业科学,2011,48(8):1438-1443.
[26] 林朝朋,芮汉明,许晓春.墨旱莲黄酮类提取物抗自由基作用及体内抗氧化功能的研究[J].军事医学科学院院刊,2005,29(4):344-345,362.
[27] 李牧.五味子提取物对断奶仔猪生产性能与抗氧化机能的影响[D].硕士学位论文.哈尔滨:东北农业大学,2007.
[28] 黄儒强,李娘辉,黄科礼,等.山稔子黄酮类提取物抗自由基作用及体内抗氧化功能的研究[J].食品科学,2008,29(9):588-590.
[29] 蔡少妃,黄春荣.大豆异黄酮对去卵巢大鼠血清SOD、T-AOC及MDA的影响[J].中国生化药物杂志,2011,32(4):315-316.

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