Molecular Nutrition

Effects of Flavonoids from Allium mongolicum Regel on Transformation Ratio and Apoptosis Ratio in Peripheral Blood Lymphocytes of Meat Sheep

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  • 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Department of Agronomy, Hetao College, Linhe 015000, China

Received date: 2016-09-13

  Online published: 2017-03-09

Abstract

This experiment was conducted to study the effects of flavonoids from Allium mongolicum Regel on transformation ratio and apoptosis ratio in peripheral blood lymphocytes of meat sheep. Sixty 6-month-old meat sheep with similar body weight[(39.9±3.2) kg] were randomly assigned to 4 groups with 15 sheep per group. Control group was fed a basal diet, and the 3 experimental groups were fed the basal diet supplemented with 11, 22 and 33 mg/kg flavonoids from Allium mongolicum Regel, respectively. Preliminary feeding period was 15 d, and the experimental period was 60 d. Blood was collected on the 60th day of experimental period before feeding via jugular venipuncture. Transformation ratio of lymphocytes was determined by thiazolyl blue method, and cell cycle and cell apoptosis ratio were determined using flow cytometry. The results showed as follows:1) dietary supplementation of 11, 22 and 33 mg/kg flavoniods from Allium mongolicum Regel could increase transformation ratio in peripheral blood lymphocytes, and 33 mg/kg group was significantly higher than control group (P<0.05); 2) compared with control group, dietary supplementation of 33 mg/kg flavoniods from Allium mongolicum Regel could significantly increase cell percentage in S phase and G2/M phase (P<0.05); 3) compared with control group and 11 mg/kg group, dietary supplementation of 22 and 33 mg/kg flavoniods from Allium mongolicum Regel could significantly increase apoptosis ratio in peripheral blood lymphocytes (P<0.05). In conclusion, dietary supplementation of 11 to 33 mg/kg flavonoids from Allium mongolicum Regel can promote transformation of peripheral blood lymphocytes of meat sheep, stimulate cell transformation to DNA synthesis period, promote cell mitosis, and had significant promotion effect on cell apoptosis, especially for the supplementation of 33 mg/kg flavonoids from Allium mongolicum Regel, which had the best effect.

Cite this article

Muqier, WANG Gui, Aochangjin, WANG Terigele, CHEN Renwei, Saruli . Effects of Flavonoids from Allium mongolicum Regel on Transformation Ratio and Apoptosis Ratio in Peripheral Blood Lymphocytes of Meat Sheep[J]. Chinese Journal of Animal Nutrition, 2017 , 29(3) : 866 -873 . DOI: 10.3969/j.issn.1006-267x.2017.03.017

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