Effects of Low Molecular Weight Chitooligosaccharides on Performance, Egg Quality, Serum Biochemical Indices, Cecal Microbial Number and Gene Expressions of Interleukin-2 and Tumor Necrosis Factor-&alpha; in Spleen of Laying Hens

MENG Xiao, WANG Jiting, WAN Wenju, WANG Hongwei, LIU Hanliang, SUN Minmin

doi:10.3969/j.issn.1006-267x.2017.05.018

2017 , Vol. 29 >Issue 5: 1590 - 1599

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.018

Molecular Nutrition

Effects of Low Molecular Weight Chitooligosaccharides on Performance, Egg Quality, Serum Biochemical Indices, Cecal Microbial Number and Gene Expressions of Interleukin-2 and Tumor Necrosis Factor-α in Spleen of Laying Hens

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1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China;
2. Department of Basic Medicine, Taishan Medical College, Tai'an 271000, China

Received date: 2016-11-01

Online published: 2017-05-13

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Abstract

This experiment was conducted to study the effects of dietary supplemented different levels low molecular weight (1 000 u) chitooligosaccharides (COS) on performance, egg quality, serum biochemical indices, cecal microbial number and gene expressions of interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) in spleen of laying hens. A total of 600 Hy-line brown laying hens (58 weeks of age) with the similar body weight and egg production rate were randomly assigned to 4 groups with 5 replicates per group and 30 hens per replicate. Hens in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diet supplemented with 300, 600 and 900 mg/kg COS, respectively. The preliminary period lasted for 7 days and the formal period lasted for 42 days. The results showed as follows: 1) compared with the control group, the supplementation of 300, 600 and 900 mg/kg COS group improved egg production rate by 4.52% (P<0.05), 2.99% (P>0.05) and 4.08% (P>0.05), respectively. 2) Compared with the control group, the supplementation of 600 and 900 mg/kg COS group improved Haugh units by 6.87%, 6.69% and 6.47%, 6.60% at third and sixth weeks end of the experiment (P<0.05), respectively. 3) Compared with the control group, dietary ssupplementation of 300 and 600 mg/kg COS significantly decreased the glucose, triglyceride contents and glutamic-oxalacetic transaminase activity in serum (P<0.05). 4) Compared with the control group, dietary supplementation of 600 and 900 mg/kg COS significantly increased the cecal Bifidobacteria and Lactobacilli number (P<0.05), and significantly decreased the cecal S. aureus. number (P<0.05). 5) Compared with the control group, dietary supplementation of 300 and 600 mg/kg COS significantly increased the mRNA expression level of IL-2 in spleen (P<0.05), and dietary supplementation of 600 mg/kg COS significantly increased the mRNA expression level of TNF-α in spleen (P<0.05). In conclusion, dietary supplementation of different levels COS increase egg production rate and Haugh unit, modulate the cecal microbial community, boost the immune ability of laying hens. The recommended COS supplemental level is 600 mg/kg.

Key words： laying hens; chitooligosaccharides; egg production; intestinal microbial community; IL-2; TNF-α

Cite this article

MENG Xiao, WANG Jiting, WAN Wenju, WANG Hongwei, LIU Hanliang, SUN Minmin . Effects of Low Molecular Weight Chitooligosaccharides on Performance, Egg Quality, Serum Biochemical Indices, Cecal Microbial Number and Gene Expressions of Interleukin-2 and Tumor Necrosis Factor-α in Spleen of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1590 -1599 . DOI: 10.3969/j.issn.1006-267x.2017.05.018

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