Molecular Nutrition

Effects of Lactic Acid Bacteria and Cellulase on Alfalfa Silage Quality with Different Moisture

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  • College of Animals Science and Technology, Northwest A&F University, Yangling 712100, China

Received date: 2016-11-15

  Online published: 2017-05-13

Abstract

To solve the difficulty of alfalfa silage and improve the quality of silage, the study was conducted to investigate effects of moisture and additive on alfalfa silage quality. Used the two factor (moisture×additive) completely random design, the moisture was 70% and 60%, respectively; the additive divided into 4 groups, control group without additives, and the other three group contained three levels which were the lactic acid bacteria group (LA group; 3, 6 and 9 mg/kg), cellulase group (CE group, 25, 50 and 100 mg/kg) and mixture of lactic acid bacteria and cellulose group (LA×CE group; 3×25, 6×50 and 9×100 mg/kg), each group contained 3 replicates, altogether 20 groups. After silage for 90 days, the content of crude protein (CP), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF) and water soluble carbohydrate (WSC) and pH, ammonia nitrogen/total nitrogen (AN/TN) were determined. The results showed as follows: compared with the control group, the silage WSC content in all LA groups was significantly increased (P<0.05), and the silage pH was significantly decreased (P<0.05); the content of CP and WSC of silage in 6 mg/kg LA group was higher than that in 3 and 9 mg/kg LA groups, and the pH was lower than that in 3 and 9 mg/kg LA groups. Compared with the control group, the ADF content and pH of silage in all CE groups was significantly decreased (P<0.05), and the ADF content and pH of silage in 50 mg/kg CE group was lower than those in 25 and 100 mg/kg CE groups. The CF and ADF content and pH of silage in all LA×CE groups most lower than those in all LA and CE groups, and the WSC content was higher than that in all LA and CE groups, the CF, ADF and WSC content and pH of silage in 6×50 mg/kg LA×CE group had significant difference compared with all LA and CE groups (P<0.05). The silage CP content in 70% moisture under the same additive were significant lower than that in 60% moisture except 9 mg/kg LA group and 100 mg/kg CE group (P<0.05); the silage CF and ADF content in 70% moisture under the same additive were significant lower than those in 60% moisture (P<0.05). In conclusion, lactic acid bacteria, cellulase and their mixture can apparently improve the quality of alfalfa silage, and the supplementation of 6×50 mg/kg lactic acid bacteria and cellulase has the best effects, meanwhile, the silage effect of 60% moisture is superior to 70% moisture.

Cite this article

ZHONG Shu, ZHANG Xiaona, YANG Yungui, YANG Yuxin . Effects of Lactic Acid Bacteria and Cellulase on Alfalfa Silage Quality with Different Moisture[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1821 -1830 . DOI: 10.3969/j.issn.1006-267x.2017.05.043

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