Short-Term Adding High-Level Oat &beta;-Glucan, Microcrystalline Cellulose and Their Mixture in Diets Affects Growth Performance, Organ Indexes and Fecal Bacterial Community Structure of Mice

ZHANG Ling, CHEN Daiwen, YU Bing, HE Jun, YU Jie, LUO Junqiu, MAO Xiangbing, HUANG Zhiqing, ZHENG Ping, LUO Yuheng

doi:10.3969/j.issn.1006-267x.2017.07.024

2017 , Vol. 29 >Issue 7: 2407 - 2415

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.07.024

Molecular Nutrition

Short-Term Adding High-Level Oat β-Glucan, Microcrystalline Cellulose and Their Mixture in Diets Affects Growth Performance, Organ Indexes and Fecal Bacterial Community Structure of Mice

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Key Laboratory for Animal Disease-Resistance Nutrition of China, Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2016-12-15

Online published: 2017-07-05

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Abstract

This experiment was conducted to investigate the effects of adding high-level oat β-glucan, microcrystalline cellulose as well as their mixture in diets on the growth performance, organ indexes and fecal bacterial community structure of mice with a short-term feeding experiment. Thirty-six healthy BALB/c mice with the body weight of (17.95±0.95) g were selected and randomly allocated to four groups according to body weight, and each group had nine mice. Mice in control group (CON group) were fed a diet without oat β-glucan and microcrystalline cellulose, while mice in the other three groups were fed diets with 28% oat β-glucan (G group), 20% microcrystalline cellulose (M group), and 14% oat β-glucan+10% microcrystalline cellulose (GM group), respectively. Each animal was raised in a single cage, and the experiment lasted for 21 days. The results showed as follows: 1) during the whole experimental period, the average daily gain (ADG) of mice showed no significant difference among groups (P>0.05), but the average daily feed intake (ADFI) of mice showed significantly different among groups (P<0.05), and the trend was presented as G groupP>0.05). The epididymal fat index of mice showed no significant difference between CON group and the three fiber supplemented groups (G, M and GM groups) (P>0.05), but it showed significantly lower in G and M groups than GM group (P<0.05). 3) Bacterial Shannon-Wiener index of feces of mice in G group was significantly lower than that in CON group (P<0.05). According the cluster analysis of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profile, fecal bacterial community structure of mice had obvious difference in among groups, fecal samples from each group could be clustered into separate clade on experimental day 13 and 17. In conclusion, short-term adding high-level oat β-glucan, microcrystalline cellulose or their mixture in diets can decrease the ADFI of mice, bur with no effect on the ADG and spleen index. The supplementation of oat β-glucan and microcrystalline cellulose mixture stimulates the epididymal fat deposition rather than the supplementation of single-type fiber. The high-level oat β-glucan can decrease the bacterial diversity in the hindgut of mice. Both of the oat β-glucan and microcrystalline cellulose can alter the fecal microflora of mice, indicating that there may be different core bacterial groups specifically utilizing the two types of dietary fibers.

Key words： oat β-glucan; microcrystalline cellulose; BABL/c mice; growth performance; organ indexes; fecal microflora

Cite this article

ZHANG Ling, CHEN Daiwen, YU Bing, HE Jun, YU Jie, LUO Junqiu, MAO Xiangbing, HUANG Zhiqing, ZHENG Ping, LUO Yuheng . Short-Term Adding High-Level Oat β-Glucan, Microcrystalline Cellulose and Their Mixture in Diets Affects Growth Performance, Organ Indexes and Fecal Bacterial Community Structure of Mice[J]. Chinese Journal of Animal Nutrition, 2017 , 29(7) : 2407 -2415 . DOI: 10.3969/j.issn.1006-267x.2017.07.024

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