Effects of Different Long-Chain Fatty Acids on Protozoal Community Structure and Bacterial Recycling Due to Protozoa Engulfment of Goat Rumen in Vitro

JING Yujia, GAO Jian, WANG Mengzhi, OUYANG Jialiang

doi:10.3969/j.issn.1006-267x.2017.08.013

2017 , Vol. 29 >Issue 8: 2707 - 2715

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.08.013

Molecular Nutrition

Effects of Different Long-Chain Fatty Acids on Protozoal Community Structure and Bacterial Recycling Due to Protozoa Engulfment of Goat Rumen in Vitro

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2017-03-07

Online published: 2017-08-02

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Abstract

This experiment was conducted to investigate the effects of different long-chain fatty acids on protozoal community structure and bacterial recycling due to protozoa engulfment of goat rumen in vitro. Six long-chain fatty acids with different saturations were selected, they were stearic acid (group A), oleic acid (group B), linoleic acid (group C), α-linoleic acid (group D), arachidonic acid (group E) and eicosapentaenoic acid (group F), respectively, and the number of unsaturated bond of them was orderly increased from 0 to 5. The fatty acids above were used at a level of 3% in the substrate to perform the in vitro culture. The protozoal engulfing bacteria rate was assayed at 10 h of the in vitro cultured and the densities of protozoal and bacterial and the contents of the protozoal protein and bacterial protein were measured at 24 h of the in vitro culture. The results showed that, except the percentage of Ophryoscolecinae in group F was significantly higher than that in groups B and D (P<0.05), adding different long-chain fatty acids had no significant effects on the percentages of Dasytricharuminantium, Entodinium, Diplodiniinae, Isotrichidae and Epidinium (P>0.05). The protozoal density was the highest in group A which was significantly higher than that in group B (P<0.05); while the bacterial density in group D was the highest and significantly higher than at in groups A, B, E and F (P<0.05). The bacteria engulfing rate of protozoa in 5 groups was 244.50 (group A), 236.51 (group B), 229.60 (group C), 189.04 (group D), 200.51 (group E), 174.24 cells/(cell·h) (group F), respectively. Additionally, the bacteria recycling rate was the lowest in group D with the longest bacterial cell recycling time (146.92 h). It was further calculated that, the bacterial protein recycling amounts were comparatively lower in groups D [126.75 mg/(d·head)] and F [131.63 mg/(d·head)]. Base on this experiment, it is therefore considered that α-linoleic acid has better inhibitory effect on the bacterial recycling due to protozoa engulfment of goat rumen in vitro.

Key words： fatty acids; unsaturated bond; protozoa; engulfing bacteria

Cite this article

JING Yujia, GAO Jian, WANG Mengzhi, OUYANG Jialiang . Effects of Different Long-Chain Fatty Acids on Protozoal Community Structure and Bacterial Recycling Due to Protozoa Engulfment of Goat Rumen in Vitro[J]. Chinese Journal of Animal Nutrition, 2017 , 29(8) : 2707 -2715 . DOI: 10.3969/j.issn.1006-267x.2017.08.013

References

[1] CARDOZO P W,CALSAMIGLIA S,FERRET A,et al.Effects of natural plant extracts on ruminal protein degradation and fermentation profiles in continuous culture[J].Journal of Animal Science,2004,82(11):3230-3236.

[2] SANTOS A B,PEREIRA M L A,SILVA H G O,et al.Nitrogen metabolism in lactating goats fed with diets containing different protein sources[J].Asian-Australasian Journal of Animal Sciences,2014,27(5):658-666.

[3] COLEMAN.The interrelationship between rumen ciliate protozoa and bacteria[M]//MCDONALD I W,WARNER A C I.Digestion and metabolism in the ruminant.Armidale:University of New England Publishing Unit,1975:149-164.

[4] 王梦芝,王洪荣,李国祥,等.用荧光染色法研究山羊瘤胃原虫对细菌吞噬速率的初报[J].中国农业科学,2008,41(5):1476-1481.

[5] JOUAN J P.Effect of rumen protozoa on nitrogen utilization by ruminants:altering ruminal nitrogen metabolism to improve protein utilization[J].The Journal of Nutrition,1996,126(4):1335S-1336S.

[6] FRIKINS J L,YU Z,MORRISON M.Ruminal nitrogen metabolism:perspectives for integration of microbiology and nutrition for dairy[J].Journal of Dairy Science,2007,90(Suppl.1):E1-E16.

[7] KOENIG K M,NEWBOLD C J,MCINTOSH F,et al.Effects of protozoa on bacterial nitrogen recycling in the rumen[J].Journal of Animal Science,2000,78(9):2431-2445.

[8] ZHANG C M,GUO Y Q,YUAN Z P,et al.Effect of octadeca carbon fatty acids on microbial fermentation,methanogenesis and microbial flora in vitro[J].Animal Feed Science and Technology,2008,146(3/4):259-269.

[9] STERN M D,VARGA G A,CLARK J H,et al.Evaluation of chemical and physical properties of feeds that affect protein metabolism in the rumen[J].Journal of Dairy Science,1994,77(9):2762-2786.

[10] FRIKINS J L.Maximizing microbial protein synthesis in the rumen:altering ruminal nitrogen metabolism to improve protein utilization[J].The Journal of Nutrition,1996,126(4):1347S-1354S.

[11] JAL ? D,?EREŠÑÁKOVÁ Z.Effect of plant oils and malate on rumen fermentation in vitro[J].Czech Journal of Animal Science,2002,47(3):106-111.

[12] JOHNSON K A,KINCAID R L,WESTBERG H H,et al.The effect of oilseeds in diets of lactating cows on milk production and methane emissions[J].Journal of Dairy Science,2002,85(6):1509-1515.

[13] 王曙,王梦芝,卢占军,等.不同植物油脂对体外培养条件下培养液酶活及微生物活力的影响[J].动物营养学报,2011,23(8):1309-1316.

[14] 拟豪杰,钱金华,李丹枫,等.不同油脂对瘤胃原虫、细菌蛋白质及DNA影响的研究[J].中国畜牧杂志,2011,47(11):33-37.

[15] JENKINS T C.Lipid metabolism in the rumen[J].Journal of Dairy Science,1993,76(12):3851-3863.

[16] BACH A,STERN M D.Effects of different levels of methionine and ruminally undegradable protein on the amino acid profile of effluent from continuous culture fermenters[J].Journal of Animal Science,1999,77(12):3377-3384.

[17] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28:7-55.

[18] 卢德勋,谢崇文.现代反刍动物营养研究方法和技术[M].北京:北京农业出版社,1991.

[19] NSABIMANA E,KIŠIDAYOVÁ S,MACHEBOEUF D,et al.Two-step freezing procedure for cryopreservation of rumen ciliates,an effective tool for creation of a frozen rumen protozoa bank[J].Applied and Environmental Microbiology,2003,69(7):3826-3832.

[20] WANG M Z,WANG H R,LI G X,et al.The preliminary report on rumen protozoa grazing rate on bacteria with a fluorescence-labeled technique[J].Agricultural Sciences in China,2008,7(6):768-774.

[21] SHERR B F,SHERR E B,FALLON R D.Use of monodispersed, fluorescently labeled bacteria to estimate in situ protozoan bacterivory[J].Applied and Environment Microbiology,1987,53:958-965.

[22] 李洪波,肖天,赵三军,等.海洋异养浮游细菌参数的测定和估算[J].海洋科学,2005,29(2):58-63.

[23] 王家玲,李顺鹏.微生物的生长与代谢[M]//王家玲.环境微生物学.2版.北京:高等教育出版社,2004:45-60.

[24] WALLACE R J,CHANDHARY L C,MCKAIN N,et al.Biohydrogenation of fatty acids in the Rumen:how,why and how to change it[C]//Proceedings of the 5th Joint Symposium of Japan-Korea-China on Rumen Metabolism and Physiology.2005:2-11.

[25] IVAN M,MIR P S,KOENIG K M,et al.Effects of dietary sunflower seed oil on rumen protozoa population and tissue concentration of conjugated linoleic acid in sheep[J].Small Ruminant Research,2001,41(3):215-227.

[26] WRIGHT D E.Hydrogenation of lipids by rumen protozoa[J].Nature,1959,184(4690):875-876.

[27] WRIGHT D E.Hydrogenation of chloroplast lipids by rumen bacteria[J].Nature,1960,185(4712):546-547.

[28] SINGH S,HAWK J C.The in vitro lipolysis and biohydrogenation of monogalactosyldigly ceride by whole rumen contents and its fractions[J].Journal of the Science of Food and Agriculture,1979,30(6):603-612.

[29] KEWELOH H,HEIPIEPER H J.Trans unsaturated fatty acids in bacteria[J].Lipids,1996,31(2):129-137.

[30] 王洪荣,秦韬,王超.青蒿素对山羊瘤胃发酵和微生物氮素微循环的影响[J].中国农业科学,2014,47(24):4904-4914.

[31] 王梦芝.山羊瘤胃原虫与细菌吞噬关系和微生物AA变化机制的研究[D].博士学位论文.扬州:扬州大学,2008.

[32] 张苏江.塔里木盆地粗饲料资源研究与利用[M].北京:中国农业科学技术出版社,2013.

[33] OLDICK B S,FIRKINS J L.Effects of degree of fat saturation on fiber digestion and microbial protein synthesis when diets are fed twelve times daily[J].Journal of Animal Science,2000,78(9):2412-2420.

[34] JOUANY J P,USHIDA K.The role of protozoa in feed digestion review[J].Asian-Australasian Journal of Animal Science,1999,12(1):113-128.

[35] DIJKSTRA J,GERRITS W J J,BANNINK A,et al.Modelling lipid metabolism in the rumen[M]//MCNAMARA J P,FRANCE J,BEEVER D E.Modelling nutrient utilization in farm animals.Egham:CABI Publishing,2000:25-36.

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