Molecular Nutrition

Effects of Dietary Flaxseed and Fish Oil on n-3 Polyunsaturated Fatty Acid Content in Egg Yolk and Fatty Acid Metabolism in Liver of Hens

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  • 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. Zhejiang Egg Bioscience Co., Inc., Changxing 313100, China

Received date: 2017-03-31

  Online published: 2017-08-02

Abstract

This experiment was conducted to investigate the effects of dietary flaxseed and fish oil on n-3 polyunsaturated fatty acid content in egg yolk, fatty acid composition and fatty acid biosynthesis gene expression in liver of hens. Ninety-six Hyline Brown hens were divided into 4 groups with 24 hens per group. Hens in the control group were fed a basal diet, and those in trial groups were fed the basal diet+10% flaxseed, the basal diet+10% flaxseed+5% fish oil, and the basal diet+5% fish oil, respectively. Eggs were collected for 7 days after 21 days feeding, and then 4 hens in each group were slaughtered for samples. The results showed as follows, compared with control group, 1) dietary flaxseed and fish oil significantly increased the n-3 polyunsaturated fatty acid contents in yolk (P<0.05), and contents of docosahexaenoic acid (DHA) and timnodonic acid were the highest in yolk affected by dietary fish oil. 2) The liver monounsaturated fatty acid ratios were significantly decreased (P<0.05), and the liver n-3 polyunsaturated fatty acid ratios were significantly increased (P<0.05) in all trial groups. 3) Dietary fish oil significantly decreased gene expressive levels of ELOVL fatty acid elongase 1, ELOVL fatty acid elongase 2 and fatty acid desaturase such as fatty acid desaturase 1, fatty acid desaturase 2 and stearoyl CoA desaturase 1 (P<0.05). In conclusion, dietary flaxseed or fish oil can concentrate n-3 polyunsaturated fatty acid content in yolk of hens. Dietary flaxseed can promote generation process from α-linolenic acid to DHA, exhibiting up-regulating expressive levels of fatty acid elongase and desaturase genes. Dietary fish oil presented contrary results to flaxseed.

Cite this article

DENG Bo, MEN Xiaoming, ZHU Dongrong, TAO Xin, YAO Yunxin, XU Ziwei . Effects of Dietary Flaxseed and Fish Oil on n-3 Polyunsaturated Fatty Acid Content in Egg Yolk and Fatty Acid Metabolism in Liver of Hens[J]. Chinese Journal of Animal Nutrition, 2017 , 29(8) : 2751 -2761 . DOI: 10.3969/j.issn.1006-267x.2017.08.018

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