Effects of Dietary Mulberry Leaf Flavonoids on Growth Performance, Body Composition, Antioxidant Indices and Resistance to Nitrite Exposure of Genetic Improvement of Farmed Tilapia (Oreochromis niloticus)

YANG Jihua, CHEN Bing, HUANG Yanhua, CAO Junming, WANG Guoxia, SUN Yuping, CHEN Xiaoying

doi:10.3969/j.issn.1006-267x.2017.09.046

2017 , Vol. 29 >Issue 9: 3403 - 3412

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.046

Molecular Nutrition

Effects of Dietary Mulberry Leaf Flavonoids on Growth Performance, Body Composition, Antioxidant Indices and Resistance to Nitrite Exposure of Genetic Improvement of Farmed Tilapia (Oreochromis niloticus)

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1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. College of Marine Science, South China Agricultural University, Guangzhou 510642, China;
3. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
4. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2017-03-01

Online published: 2017-09-08

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Abstract

This experiment was conducted to investigate the effects of dietary mulberry leaf flavonoids (MLF) on growth performance, body composition, serum and liver antioxidant indices and resistance to nitrite exposure of juvenile genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus). A total of 840 GIFT with an initial body weight of (1.51±0.02) g were randomly divided into 6 groups with 4 replicates per group and 35 fish per replicate. Fish in the control group were fed a basal diet, and the others in the experimental groups were fed basal diets supplemented with 50, 100, 300, 500 and 1 000 mg/kg MLF, respectively. The feeding trial lasted for 56 days. After the feeding trial, sodium nitrite was used to conduct a nitrite exposure trial in each group for 72 h. The results showed as follows:1) there were no significant differences on the weight gain ratio (WGR), feed conversion ratio (FCR) and protein efficiency rate (PER) of GIFT among all groups. (P>0.05). There were no significant differences in the crude protein, crude lipid, ash and moisture contents of GIFT whole body among all groups (P>0.05). 2) The serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and total antioxidant (T-AOC) in experimental groups were higher than in control group. The serum SOD activities in 100, 300, 500, and 1 000 mg/kg MLF groups were significantly higher than those in control group (P<0.05), the serum GSH-Px activities in 50, 500 and 1 000 mg/kg MLF groups were significantly higher than those in control group (P<0.05), the serum T-AOC in 100 and 500 mg/kg MLF groups were significantly higher than those in control group (P<0.05). Compared with the control group, the serum malondialdehyde (MDA) contents in experimental groups were significantly decreased (P<0.05). The liver SOD, catalase (CAT) activities and T-AOC in 100, 300 and 500 mg/kg MLF groups were significantly higher than those in control group (P<0.05). The highest value of liver SOD activity was observed in 500 mg/kg MLF group, and significantly higher than that in 1 000 mg/kg MLF group (P<0.05). 3) After 48 and 72 h nitrite exposure, the cumulative mortality rates in 50, 100 and 500 mg/kg MLF groups were significant lower than those in control group (P<0.05). In conclusion, dietary MLF do not significantly affect the growth performance, but improve the serum and liver antioxidant indices and resistance to nitrite exposure of GIFT. Under the experimental conditions, by analysis of the regression equation, the optimal supplemental level of MLF in the diet of juvenile GIFT is 100 mg/kg basis of SOD activity and T-AOC in serum; the optimal supplemental level of MLF in the diet of juvenile GIFT is 371.00 to 441.75 mg/kg basis of SOD activity and T-AOC in liver.

Key words： mulberry leaf flavonoids; Oreochromis niloticus; growth performance; antioxidant; nitrite

Cite this article

YANG Jihua, CHEN Bing, HUANG Yanhua, CAO Junming, WANG Guoxia, SUN Yuping, CHEN Xiaoying . Effects of Dietary Mulberry Leaf Flavonoids on Growth Performance, Body Composition, Antioxidant Indices and Resistance to Nitrite Exposure of Genetic Improvement of Farmed Tilapia (Oreochromis niloticus)[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3403 -3412 . DOI: 10.3969/j.issn.1006-267x.2017.09.046

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