Selenium Enhances Anti-Inflammatory Effects of Docosahexaenoic Acid on Inflammatory Response in Macrophages Induced by Lipopolysaccharide

XU Qipin, HUANG Yixin, LUO Zhengzhong, SHEN Liuhong, YAO Xueping, YU Shumin, CAO Suizhong

doi:10.3969/j.issn.1006-267x.2017.10.021

2017 , Vol. 29 >Issue 10: 3598 - 3603

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.021

Molecular Nutrition

Selenium Enhances Anti-Inflammatory Effects of Docosahexaenoic Acid on Inflammatory Response in Macrophages Induced by Lipopolysaccharide

Expand

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2017-03-29

Online published: 2017-09-28

Fold

Abstract

In order to investigate whether selenium (Se) affects anti-inflammatory action of docosahexaenoic acid (DHA) on lipopolysaccharide (LPS) induced-macrophage inflammatory response. RAW264.7 cells of mice macrophagic system were treated with 10 μg/mL DHA, 10 μg/mL DHA+0.05 μmol/L sodium selenite, 1 μg/mL LPS, 10 μg/mL DHA+1 μg/mL LPS, 10 μg/mL DHA+1 μg/mL LPS+0.05 μmol/L sodium selenite for 24 h, respectively, a normal group was set up without addition. The mRNA expression levels of tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), interleukin 6 (IL-6) and interleukin 10 (IL-10) in cells were measured by semi-quantitative reverse transcription PCR, and enzyme linked immunosorbent assay (ELISA) method was used to test contents of TNF-α, IL-1β, IL-6 and IL-10 in supernatant of culture medium. The results showed that adding sodium selenite (0.05 μmol/L) not only significantly enhanced the inhibitory effects of DHA (10 μg/mL) on expression of proinflammatory cytokines TNF-α mRNA (P<0.05) and production of IL-1β (P<0.01), but also significantly enhanced the promoting effects of DHA (10 μg/mL) on mRNA expression of anti-inflammatory cytokine IL-10 in RAW264.7 cells induced by LPS (1 μg/mL) (P<0.01). Therefore, Se can enhance anti-inflammatory effects of DHA on inflammatory response in macrophages induced by LPS.

Key words： DHA; selenium; macrophages; anti-inflammatory effect

Cite this article

XU Qipin, HUANG Yixin, LUO Zhengzhong, SHEN Liuhong, YAO Xueping, YU Shumin, CAO Suizhong . Selenium Enhances Anti-Inflammatory Effects of Docosahexaenoic Acid on Inflammatory Response in Macrophages Induced by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2017 , 29(10) : 3598 -3603 . DOI: 10.3969/j.issn.1006-267x.2017.10.021

References

[1] ALESSANDRI A L,SOUSA L P,LUCAS C D,et al.Resolution of inflammation:mechanisms and opportunity for drug development[J].Pharmacology & Therapeutics,2013,139(2):189-212.

[2] MARION-LETELLIER R,SAVOYE G,GHOSH S.Polyunsaturated fatty acids and inflammation[J].IUBMB Life,2015,67(9):659-667.

[3] HOFFMANN P R,BERRY M J.The influence of selenium on immune responses[J].Molecular Nutrition & Food Research,2008,52(11):1273-1280.

[4] 于昱,吕林,张亿一,等.硒对硒蛋白-谷胱甘肽过氧化物酶基因表达及其酶活性的调节[J].动物营养学报,2007,19(S1):469-474.

[5] ZARCZY?SKA K,SOBIECH P,RADWINSKA J,et al.Effects of selenium on animal health[J].Journal of Elementology,2013,18(2):329-340.

[6] CHENG A W M,STABLER T V,BOLOGNESI M,et al.Selenomethionine inhibits IL-1β inducible nitric oxide synthase (iNOS) and cyclooxygenase 2(COX2) expression in primary human chondrocytes[J].Osteoarthritis and Cartilage,2011,19(1):118-125.

[7] BOOSALIS M G.The role of selenium in chronic disease[J].Nutrition in Clinical Practice,2008,23(2):152-160.

[8] YANG D J,LIN J T,CHEN Y C,et al.Suppressive effect of carotenoid extract of Dunaliella salina alga on production of LPS-stimulated pro-inflammatory mediators in RAW264.7 cells via NF-κB and JNK inactivationc[J].Journal of Functional Foods,2013,5(2):607-615.

[9] LIN W W,KARIN M.A cytokine-mediated link between innate immunity,inflammation,and cancer[J].Journal of Clinical Investigation,2007,117(5):1175-1183.

[10] RAHMAN M M,BHATTACHARYA A,FERNANDES G.Docosahexaenoic acid is more potent inhibitor of osteoclast differentiation in RAW 264.7 cells than eicosapentaenoic acid[J].Journal of Cellular Physiology,2008,214(1):201-209.

[11] WELDON S M,MULLEN A C,LOSCHER C E,et al.Docosahexaenoic acid induces an anti-inflammatory profile in lipopolysaccharide-stimulated human THP-1 macrophages more effectively than eicosapentaenoic acid[J].The Journal of Nutritional Biochemistry,2007,18(4):250-258.

[12] MULLEN A,LOSCHER C E,ROCHE H M.Anti-inflammatory effects of EPA and DHA are dependent upon time and dose-response elements associated with LPS stimulation in THP-1-derived macrophages[J].The Journal of Nutritional Biochemistry,2009,21(5):444-450.

[13] HONDA K L,LAMON-FAVA S,MATTHAN N R,et al.Docosahexaenoic acid differentially affects TNFα and IL-6 expression in LPS-stimulated RAW 264.7 murine macrophages[J]. Prostaglandins, Leukotrienes and Essential Fatty Acids,2015,97:27-34.

[14] SOLANKI P,AMINOSHARIAE A,JIN G,et al.The effect of docosahexaenoic acid (DHA) on expression of IL-1β,IL-6,IL-8,and TNF-α in normal and lipopolysaccharide (LPS)-stimulated macrophages[J].Quintessence International,2013,44(6):393.

[15] VUNTA H,BELDA B J,ARNER R J,et al.Selenium attenuates pro-inflammatory gene expression in macrophages[J].Molecular Nutrition & Food Research,2008,52(11):1316-1323.

[16] MATTMILLER S A,CARLSON B A,CANDY J C,et al.Reduced macrophage selenoprotein expression alters oxidized lipid metabolite biosynthesis from arachidonic and linoleic acid[J].Journal of Nutritional Biochemistry,2014,25(6):647-654.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References