Effects of Inulin Supplemental Level on Microbial Community Structure and Main Microbial Populations in Cecum and Rectum of Broilers

YANG Guiqin, WANG Qi, LIU Haiying, DONG Weiguo, ZHU Xin

doi:10.3969/j.issn.1006-267x.2017.12.021

2017 , Vol. 29 >Issue 12: 4408 - 4418

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.12.021

Molecular Nutrition

Effects of Inulin Supplemental Level on Microbial Community Structure and Main Microbial Populations in Cecum and Rectum of Broilers

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College of Animal Husbandry and Veterinary, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2017-06-05

Online published: 2017-12-04

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Abstract

The purpose of this experiment was to study the effects of inulin supplemental level on microbial community structure and main microbial populations in cecum and rectum of broilers. Three hundred 1-day-old Arbor Acres broilers were selected and randomly distributed into five groups with six replicates per group and ten broilers per replicate by single-factor completely random design. Broilers in those five groups were fed a basal diet supplemented with 0 (control group), 0.5, 1.5, 2.5 and 5.0 g/kg inulin, respectively. The experiment lasted for 6 weeks. The microbial community structure and the main microbial populations in cecum and rectum of 42-day-old broilers were measured using the PCR-denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) techniques. The results showed as follows:1) inulin supplemental level had no significant effects on the cecal and rectal microbial richness, the cecal microbial Shannon-Wiener index and the rectal microbial evenness of broilers (P>0.05), but significantly decreased the cecal microbial evenness and rectal microbial Shannon-Wiener index (P<0.05). Based on the analysis of some dominant bands in PCR-DGGE fingerprint, diet supplemented with inulin promoted the growth of Eubacterium coprostanoligenes, Intestinimonas sp., Enterococcus cecorum and so on from Firmicutes in cecum and rectum of broilers. 2) Diet supplemented with inulin significantly affected the populations of total bacteria and Escherichia coli in cecum and rectum of broilers (P<0.05). Quadratic curve analysis showed that the populations of total bacteria and Escherichia coli were the fewest in cecum of broilers when inulin supplemental levels were 2.71 and 2.66 g/kg, respectively. Compared with control group, diet supplemented with 1.5, 2.5 and 5.0 g/kg inulin significantly decreased the population of cecal Lactobacillus (P<0.05), while diet supplemented with 0.5 g/kg inulin significantly increased the population of rectal Lactobacillus (P<0.05). The populations of cecal and rectal Bifidobacteria and rectal Clostridium perfringens were the most when diet supplemented with 5.0 g/kg inulin, and the differences were significant compared with control group (P<0.05). Thus, results from the current study suggest that supplementation of 2.5 to 5.0 g/kg inulin to basal diet may have the beneficial effects on improving the microbial community and promoting the growth of beneficial bacteria in intestine of broilers under this experimental condition.

Key words： inulin; microbial community; microbial population; intestine; broilers

Cite this article

YANG Guiqin, WANG Qi, LIU Haiying, DONG Weiguo, ZHU Xin . Effects of Inulin Supplemental Level on Microbial Community Structure and Main Microbial Populations in Cecum and Rectum of Broilers[J]. Chinese Journal of Animal Nutrition, 2017 , 29(12) : 4408 -4418 . DOI: 10.3969/j.issn.1006-267x.2017.12.021

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