This experiment was to investigate the effects of different dietary vitamin E levels on immune function, α-tocopherol deposition and gene expressions of lipoprotein lipase (LPL) and fatty acid-binding proteins in tissues of Sanhuang chicken. A total of 256 Guangxi Sanhuang chickens at 80 days of age were randomly divided into 4 groups with 4 replicates per group and 16 chickens per replicate. Vitamin E at 0 (control), 50, 100 and 150 mg/kg was added to a basal diet of different groups, respectively. Pre-experiment lasted for 5 d, and experiment lasted for 35 d. The results showed that compared with control group:1) the content of immunoglobulin A (IgA) in serum was significantly increased by dietary supplementation of 50 mg/kg vitamin E (P<0.05); the content of immunoglobulin M (IgM) in serum was significantly increased by dietary supplementation of 50 mg/kg vitamin E (P<0.05); dietary supplementation of different levels of vitamin E had no significant effects on spleen index, and the contents of interleukin-2, tumor necrosis factor-α, interferon-γ and immunoglobulin G (IgG) in serum (P>0.05). 2) The content of α-tocopherol in liver was significantly increased by dietary supplementation of 50, 100 and 150 mg/kg vitamin D (P<0.05); the content of α-tocopherol in breast was significantly increased by dietary supplementation of 150 mg/kg vitamin D (P<0.05); dietary supplementation of different levels of vitamin E had no significant effects on the content of α-tocopherol in thigh (P>0.05). 3) The expression level of LPL gene in liver was significantly increased by dietary supplementation of 100 and 150 mg/kg vitamin D (P<0.05); the expression level of heart type-fatty acid-binding protein (H-FABP) gene in liver was significantly increased by dietary supplementation of 150 mg/kg vitamin D (P<0.05); the expression level of liver type-fatty acid-binding protein (L-FABP) gene in liver was significantly increased by dietary supplementation of 50, 100 and 150 mg/kg vitamin D (P<0.05); dietary supplementation of different levels of vitamin E had no significant effects on the expression level of adipocyte type-fatty acid-binding protein (A-FABP) gene (P>0.05). 4) There was a significant positive correlation between dietary vitamin E level and expression levels of LPL, H-FABP and L-FABP genes in liver of Guangxi Sanhuang chicken (P<0.05). In summary, it is suggested that the supplementation of a high level (150 mg/kg) of vitamin E in diet can improve immune function, increase α-tocopherol deposition in tissues, and regulate expression levels of LPL, H-FABP and L-FABP genes in liver of Guangxi Sanhuang chicken, which may has impact on the body's fat metabolism.