Molecular Nutrition

Effects of Different Combinations of Essential Oil and Organic Acid on Salmonella enteritidis Infection of Broiler Chickens

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  • 1. Shandong Key Laboratory of Animal Biotechnology and Disease Control, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
    2. Shanghai Menon Biotechnology Co., Ltd., Shanghai 201807, China

Received date: 2018-01-03

  Online published: 2018-07-20

Abstract

This experiment was conducted to investigate the effects of different combinations of essential oil and organic acid on Salmonella enteritidis (SE) infection of broiler chickens. A total of 840 1-day-old Arbor Acres broiler chickens hatchlings without SE infection were randomly allotted into 7 groups and housed in separate rooms with the same configuration, and with 6 replicates per group and 20 birds (males and females in half) per replicate. The broiler chickens in group 1 (control group) were fed a basal diet, those in group 2 (antibiotic group) were fed the basal diet supplemented with 40 g/t enrofloxacin HCl, and those in groups 3 to 7 (essential oil and organic acid groups) were fed the basal diet supplemented with 800 g/t different combinations of essential oil and organic acid (combinations A, B, C, D, E), respectively. At 14 days of age, each bird was orally inoculated with 200 μL SDBL-1 SE bacterial fluid (5×107 CFU/mL). Two birds randomly selected from each replicate were taken blood, slaughtered and sampled at 2, 7 and 14 days post-challenge (16, 21 and 28 days of age, respectively). The experiment lasted for 28 days, including 2 feeding stages of 1 to 21 days of age and 22 to 28 days of age. The results showed as follows:1) compared with control group, combination A significantly decreased the ratio of feed to gain (F/G) of broiler chickens before and after bacterial inoculation (P<0.05), whereas combinations E and D enhanced growth performance only in pre-and post-challenge period respectively (P<0.05). 2) In terms of bacterial load in tissues, combinations A and D offered a good control effects on SE infection of broiler chickens. 3) After SE infection, compared with control group, combination A significantly decreased the contents of tumor necrosis factor-α (TNF-α) in serum of broiler chickens at 28 days of age (P<0.05), and significantly increased the content of immunoglobulin G (IgG) in serum at 16 days of age (P<0.05). The reverse trend was observed for combination E. In conclusion, combinations A and D have great inhibitory effect on SE of broiler chickens, but combination A is suitable for long-term feeding, and combination D is recommended to be used only after infection.

Cite this article

ZHAO Jingpeng, LI Peiyong, WANG Hongyu, SONG Yizhen, SUN Shuhong, LIN Hai . Effects of Different Combinations of Essential Oil and Organic Acid on Salmonella enteritidis Infection of Broiler Chickens[J]. Chinese Journal of Animal Nutrition, 2018 , 30(7) : 2672 -2682 . DOI: 10.3969/j.issn.1006-267x.2018.07.027

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