Molecular Nutrition

Effects of Living Bacteria Preparation and Compound Enzymes Preparation on Chemical Composition and Microstructure of Fibers of Silage Corn Stover

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  • 1. Beijing Key Laboratory of Dairy Cow Nutrition, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Animal Science and Technology, Tarim University, Alar 843300, China

Received date: 2017-12-25

  Online published: 2018-07-20

Abstract

This study was conducted to investigate the effects of corn stover treated by enzyme preparation and enzyme-bacteria compound preparation on fermentation quality, nutrition composition and microstructure of fibers, and aimed to explore its mechanism of improving corn stover utilization by breaking the cellulose microstructure. Five groups were designed in the present study:corn stover group (CS group), silage control group (CK group), corn stover treated by compound enzyme group (CPL group), corn stover treated by compound enzyme and single living bacteria group (CPLP group), and corn stover treated by double living bacteria group (CPLB group). Samples were prepared by vacuum packager in polyethylene bags, after storing at room temperature for 45 d, the chemical composition and fermentation quality of silage corn stover were measured. Additionally, the microstructure of fibers was analyzed by phenol-sulfuric acid+4,4'-dicarboxy-2,2'-biquinoline disodium (BCA) methods, Fourier transform infared spectroscopy (FTIR), X-ray diffraction and iran methylene method. The results showed as follows:1) compared with the CK group, the pH, ratio of ammonia nitrogen to total nitrogen (NH3-N/TN) and ratio of acetic acid to lactic acid in the CPL group were significantly decreased (P<0.05), the contents of lactic acid, neutral detergent fiber (NDF), acid washing fiber (ADF), cellulose and hemicellulose in the CPL group had no significant difference (P>0.05); the content of lactic acid in the CPLB group was significantly increased (P<0.05); the pH, NH3-N/TN, ratio of acetic acid to lactic acid and contents of NDF, ADF, cellulose and hemicellulose in CPLP and CPLB groups were significantly decreased (P<0.05). 2) Compared with the CK group, the crystallinity of tertiary structure in the CPL group was significantly increased (P<0.05), the degree of polymerization of primary structure and specific surface area of quaternary structure had no significant difference (P>0.05), the intermolecular hydrogen bonding force of secondary structure did not change; the degree of polymerization of primary structure in PLP and CPLB groups were significantly decreased (P<0.05), the specific surface area of quaternary structure were significantly increased (P<0.05); the intermolecular hydrogen bonding force of secondary structure was weakened, the crystallinity of tertiary structure had no significant difference (P<0.05). In conclusion, the compound enzyme-single living bacteria and compound enzyme-double living bacteria can significantly improve the chemical composition of corn stover, it can effectively crack down the microstructure of the cell wall of corn stovers by reducing the degree of polymerization of primary structure and the amorphous area of tertiary structure, weakening the intermolecular hydrogen bonding force of secondary structure and increasing the specific surface area of quaternary structure from cellulose of corn stover, and enhancing the utilization rate of straw.

Cite this article

MAO Jianhong, TAO Lian, LIU Rong, WANG Yurong, XU Guishan, DIAO Qiyu . Effects of Living Bacteria Preparation and Compound Enzymes Preparation on Chemical Composition and Microstructure of Fibers of Silage Corn Stover[J]. Chinese Journal of Animal Nutrition, 2018 , 30(7) : 2763 -2771 . DOI: 10.3969/j.issn.1006-267x.2018.07.037

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