Effects of Different Feed Additives on Fatty Acid Composition and Oxidation Stability of Body Fat in Dorper&times;Thin-Tailed Han Crossbred Mutton Lambs

LIU Wangjing, AO Changjin, DING He, Khaserdene, DAN Ni, LI Shuyi, LI Ying, ZHANG Zuozhong

doi:10.3969/j.issn.1006-267x.2018.09.048

2018 , Vol. 30 >Issue 9: 3759 - 3771

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.048

Molecular Nutrition

Effects of Different Feed Additives on Fatty Acid Composition and Oxidation Stability of Body Fat in Dorper×Thin-Tailed Han Crossbred Mutton Lambs

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-01-29

Online published: 2018-09-20

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Abstract

The objective of this experiment was to investigate the effects of different feed additives on fatty acid composition and oxidation stability of body fat in Dorper×thin-tailed Han crossbred mutton lambs. Single factor completely randomized block design was used in the current study. A total of 30 healthy and six-month-old Dorper×thin-tailed Han crossbred F1 mutton lambs with the similar body weight[(42.5±3.1) kg] were randomly divided into 3 groups with 10 lambs each. Lambs in control group (G1 group) were fed a basal diet, those in experimental group 1 (G2 group) were fed the basal diet supplemented with Allium mongolicum Regel powder (20 g/d each lamb), and those in experimental group 2 (G3 group) were fed the basal diet supplemented with microbial fermented feed (100 g/d each lamb). The trial period was 75 d, which consisted of 15 d of per-feeding period and 60 d of formal period. At the end of feeding experiment, three lambs were randomly selected from each group and slaughtered to collect the perirenal adipose tissue, abdominal subcutaneous adipose tissue and tail adipose tissue to measure fatty acid composition, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. The results showed as follows:compared with G1 group, 1) G2 group and G3 group showed significantly higher average daily gain and significantly lower feed to gain ratio of mutton lambs (P<0.05). 2) G2 group showed significantly higher the contents of linoleic acid (C18:2cis-6), α-linolenic acid (C18:3n-3), eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3), polyunsaturated fatty acids (PUFA), n-6 PUFA and the value of PUFA/saturated fatty acid (SFA) (P<0.05) and significantly lower the content of C18:0 in the perirenal adipose tissue, abdominal subcutaneous adipose tissue and tail adipose tissue (P<0.05); G3 group showed significantly higher DHA content in the abdominal subcutaneous adipose tissue and tail adipose tissue (P<0.05), and showed significantly lower the contents of monounsaturated fatty acids (MUFA) and C18:0 in the perirenal adipose tissue (P<0.05). 3) The contents of n-6 PUFA and the value of n-6/n-3 in the tail adipose tissue in G2 group and G3 group were significantly increased (P<0.05), the content of n-3 PUFA in the perirenal adipose tissue in G2 group was significantly increased (P<0.05), and the value of PUFA/SFA in the perirenal adipose tissue in G3 group was significantly decreased (P<0.05). 4) G2 group and G3 group significantly up-regulated the SOD activity and significantly down-regulated the MDA content in the perirenal adipose tissue and abdominal subcutaneous adipose tissue (P<0.05). 5) There were linear relationships between the MDA content, SOD activity and PUFA deposition (P<0.001) in the perirenal adipose tissue (R²=0.992), abdominal subcutaneous adipose tissue (R²=0.967) and tail adipose tissue (R²=0.965). The contribution of MDA was negative for the multiple regression equation, whereas the SOD was positive. In conclusion, diet supplemented with Allium mongilicum Regel powder and microbial fermented feed both can improve the growth performance of Dorper×thin-tailed Han crossbred mutton lambs. Diet supplemented with Allium mongilicum Regel powder can improve the fatty acid composition and fat oxidative stability effectively, which is significant in improving the fat quality of Dorper×thin-tailed Han crossbred mutton lambs. Diet supplemented with microbial fermented feed cannot improve the fatty acid composition effectively, but can increase the fat oxidative stability of Dorper×thin-tailed Han crossbred mutton lambs.

Key words： feed additives; mutton lamb; fat quality; antioxidant; oxidation stability; polyunsaturated fatty acids

Cite this article

LIU Wangjing, AO Changjin, DING He, Khaserdene, DAN Ni, LI Shuyi, LI Ying, ZHANG Zuozhong . Effects of Different Feed Additives on Fatty Acid Composition and Oxidation Stability of Body Fat in Dorper×Thin-Tailed Han Crossbred Mutton Lambs[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3759 -3771 . DOI: 10.3969/j.issn.1006-267x.2018.09.048

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