Evaluation of Relative Bioavailability of Copper Source with Slope Ratio Method for Broilers

WU Xuezhuang, WEN Zhiguo, HU Hong, WEN Aiyou, HUA Jinling, WANG Lixin, WANG Shujuan, DAI Sifa, GAO Xiuhua, CAI Zhihua

doi:10.3969/j.issn.1006-267x.2019.04.016

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 4: 1596 - 1603

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.016

Molecular Nutrition

Evaluation of Relative Bioavailability of Copper Source with Slope Ratio Method for Broilers

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1. College of Animal Science, Anhui Science and Technology University, Bengbu 2331001, China;
2. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2018-09-09

Online published: 2019-04-16

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Abstract

This study was conducted to evaluate the relative bioavailability of different copper (Cu) source for broilers. According to 3×2 factorial completely randomized test design, three Cu sources (copper sulfate, tribasic copper chloride and copper methionine) and 2 Cu supplemented levels (10 and 20 mg/kg) as the two main factors, control group without Cu supplementation, and 7 groups were formed. A total of 504 1-day-old healthy male Arbor Acres (AA) broilers were randomly divided into 7 groups with 6 replicates per group and 12 broilers per replicate. The experiment lasted for 21 days. The results showed that dietary Cu source and Cu supplemented level had no significant effects on final body weight, average daily feed intake, average daily gain and the ratio of feed to gain of broilers aged from 1 to 21 days (P>0.05). The content of Cu in liver and the activity of ceruloplasmin in plasma were significantly increased with dietary Cu supplemented level increased (P<0.01). Compared with copper sulfate (100%), relative bioavailability of tribasic copper chloride and copper methionine were 141.05% and 142.07% based on the activity of ceruloplasmin in plasma as evaluating indicator, respectively; and relative bioavailability of tribasic copper chloride and copper methionine were 127.61% and 137.64% based on the content of Cu in liver as evaluating indicator, respectively. In conclusion, dietary Cu supplemented level less than 20 mg/kg (the content of Cu in basal diet is 9.65 mg/kg) has no significant growth-promoting effects on broilers aged from 1 to 21 days, and the relative bioavailability of 3 Cu sources in low-copper diets (Cu supplemented level less than 20 mg/kg) for broilers aged 21 days is copper methionine > tribasic copper chloride > copper sulfate.

Key words： Cu source; broilers; relative bioavailability

Cite this article

WU Xuezhuang, WEN Zhiguo, HU Hong, WEN Aiyou, HUA Jinling, WANG Lixin, WANG Shujuan, DAI Sifa, GAO Xiuhua, CAI Zhihua . Evaluation of Relative Bioavailability of Copper Source with Slope Ratio Method for Broilers[J]. Chinese Journal of Animal Nutrition, 2019 , 31(4) : 1596 -1603 . DOI: 10.3969/j.issn.1006-267x.2019.04.016

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