Effects of Dietary Montmorillonite and Bacillus subtilis on Cecal Microflora and Intestinal Permeability of Laying Hens

QU Xiangyong, CHEN Jifa, KUANG Youhua, KANG Kelang, TANG Shengguo, HE Changqing

doi:10.3969/j.issn.1006-267x.2019.04.048

2019 , Vol. 31 >Issue 4: 1887 - 1896

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.048

Molecular Nutrition

Effects of Dietary Montmorillonite and Bacillus subtilis on Cecal Microflora and Intestinal Permeability of Laying Hens

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Hunan Co-Innovation Center of Animal Production Safety, Hunan Engineering Research Center of Poultry Production Safety, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2018-09-30

Online published: 2019-04-16

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Abstract

This study was conducted to evaluate the effects of dietary montmorillonite (MMT) and Bacillus subtilis (BS) on cecal microflora and intestinal permeability of laying hens. The trial used a 2×2 factorial random design. A total of 360 healthy 29-week-old Lohmann pink hens were randomly arranged into 4 groups with 6 replicates per group and 15 hens in each replicate. The hens in 4 groups were fed a basal diet (control group), the basal diet+0.5 g/kg MMT (MMT group), the basal diet+5×10⁸ CFU/kg BS (BS group), and the basal diet+0.5 g/kg MMT+5×10⁸ CFU/kg BS (MMT+BS group), respectively. The adjustment period lasted for 7 days, and the experimental period lasted for 70 days. The results showed as follows:1) the quantities of effective sequences observed in the BS group were more than those in the control group, MMT group and MMT+BS group (P<0.05); the quantities of good sequences observed in experimental groups were more than those in the control group (P>0.05). 2) At the phylum level, the relative content of Fusobacteria in the MMT group tended to be lower than that in the control group (P=0.072), and the relative content of Elusimicrobia in the MMT+BS group tended to be lower than that in the control group (P=0.053). At the genus level, the relative content of Fusobacterium in the MMT group tended to be lower than that in the control group (P=0.072). Compared with the control group, the relative content of Lactobacillus in the BS group was significantly increased (P<0.05), the relative content of Megamonas in BS and MMT+BS groups was significantly increased (P<0.05), and the relative content of Clostridium in the MMT+BS group was significantly decreased (P<0.05). 3) Compared with control group, dietary BS significantly decreased the activity of diamine oxidase and endotoxin content in plasma (P<0.05), and tended to reduce plasma content of D-lactic acid (P=0.051). In conclusion, the species abundance of cecal microbiota in laying hens can be partially influenced by single or combined addition of MMT and BS, and dietary supplementation with BS decreases intestinal permeability of laying hens.

Key words： montmorillonite; Bacillus subtilis; intestinal microflora; gut permeability; laying hens

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QU Xiangyong, CHEN Jifa, KUANG Youhua, KANG Kelang, TANG Shengguo, HE Changqing . Effects of Dietary Montmorillonite and Bacillus subtilis on Cecal Microflora and Intestinal Permeability of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2019 , 31(4) : 1887 -1896 . DOI: 10.3969/j.issn.1006-267x.2019.04.048

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